Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F51 I1 R1
|
25 |
46.9 |
1811498 |
90.3% |
1635782 |
141.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,125,097 |
C→T |
E572K (GAG→AAG) |
priA ← |
primosome factor N' |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,125,097 | 0 | C | T | 95.5%
| 123.0
/ NA
| 45 | E572K (GAG→AAG) | priA | primosome factor N' |
| Reads supporting (aligned to +/- strand): ref base C (1/0); major base T (23/19); minor base G (1/1); total (25/20) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GGCAAAGGCGTCGTAGCCTTTATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTACTTCTGC > NC_000913/4124957‑4125235
|
ggCAAAGGCGTCGTAGCCTTTATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACAACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTg < 1:496192/143‑1 (MQ=255)
aGGCGTCGTAGCCTTTATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAg < 2:643281/143‑1 (MQ=255)
aGGCGCTGTGGCCTTTATAGAGCAACGCTGGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTCCCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAg < 2:794983/143‑1 (MQ=255)
cgtcgtAGCGTTTATGTCGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTGCGCCCTGTTTACCCGCAGGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGa < 2:208626/143‑1 (MQ=255)
tcgtAGCCTTTATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGaag > 1:773187/1‑142 (MQ=255)
tAGCCTTTATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATCGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGCCCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCCAAAATc > 1:401460/1‑143 (MQ=255)
ccTTTATAGAGCAACGTTTGCAACCGACGATGTTCCGGATGGTGCGTATGCAGCACCACTTCGCCCCCTTTACGCGCACGCCCGTCACGACACGCGCCACGGGTGTAAAGCTGCGCACAGCGCTCGGCCGCTCGaaccgcggg > 2:351151/1‑136 (MQ=255)
cTTTATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTCAAGCTGAGCGCAACGCTTTGCCGAGCGAAACTCCGcc > 2:439632/1‑142 (MQ=255)
tttATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCGTGTTGACCCGCACGCCGGGCGCGACCGGCCACCTGGGGGTAAAGCGGAGGGAAACGCTGCGCCGAGCAAGAATGCGCAg > 2:731777/1‑143 (MQ=255)
tttATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGGCCTGGGTGTAAAGCTGAGCGAAACGCATTGCCGAGCGAAAATCGGccg > 1:291274/1‑141 (MQ=255)
ataGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGaaa > 1:223830/1‑143 (MQ=255)
ataGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGaaa > 1:841452/1‑143 (MQ=255)
taGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGaaaa > 2:361713/1‑143 (MQ=255)
agagCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGACAATCGGCAGCAAAc > 2:540874/1‑143 (MQ=255)
aaCGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGCGTAAAGCTGAGCGCAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAgcgc > 2:482808/1‑143 (MQ=255)
aaCAGAGTATGGTCCGGATGGGGCGTTTGCAGCACCACTTCGCCCGGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCcgtccac < 1:751860/143‑1 (MQ=255)
aCAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGCTTACCCGCCCGCCCGGCACGACCGCCGCCCCGGGTGTACCGCTGGGCGAGACGCCTCGCCGCGCGCAACTCGGCAGACACCACCCCGCcccccacg > 2:102873/1‑143 (MQ=255)
ggATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAg < 1:122485/143‑1 (MQ=255)
tCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAAGCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGc > 2:24085/1‑143 (MQ=255)
cGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGCAAATCGGCCGAAAACAGCGCGCCGGCCACGTCCCGTAATGCaa > 2:233076/1‑143 (MQ=255)
cGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCaa < 1:361713/143‑1 (MQ=255)
cGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAAGCGGCAGAAAACAGCGCGCCGTCCACGTCCGGTAGTGcga > 2:210284/1‑141 (MQ=255)
gCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTc < 2:86462/143‑1 (MQ=255)
cagcaCCACTTCGCCCGGTTTACCCGCACGCCCGGCACGACCCCCGACCTGCGTGTAAAGCTGCGCGAAACGCTTTGCCGACCGCAAATCGGCCGAAAACACCCCGCCGGACCCATCACGTAATCCCACCAGCGTccgacccc > 2:238704/1‑136 (MQ=255)
accacTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCGGGaa < 2:829379/143‑1 (MQ=255)
cTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCGGGAAATgg < 1:592623/143‑1 (MQ=255)
gCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAGTGCAACCAGCGTCACATCCGGGAAATGGTGAc > 2:25925/1‑143 (MQ=255)
gCCCGGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCGGCGTCACATCCGGGAAATGGTGAc < 2:436212/143‑1 (MQ=255)
cgcTGGTTACCCGCACGGGGGGCACGACCGGCGAGTTGGGTGTAAGGGTGAGCGAAGCGCTGTGTGGAGCGAAAATCGGCAGAAAACAGCGCGCCGTGCACGTCCAGTAATGCAACCAGCGTCACATCCGGGAAATGGTGAcc < 2:12653/141‑1 (MQ=255)
ccTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCGGGAAATGGTGACCt > 2:846671/1‑143 (MQ=255)
tACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGAAGAAAACAGCGCGCCGTCCACGTGCCGTAATGCAACCAGCGTGACATCCGGGAAATGGGGACCtctgggc > 2:848322/1‑137 (MQ=255)
aCCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCa < 2:661174/143‑1 (MQ=255)
ccGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGc < 1:434308/143‑1 (MQ=255)
cGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCa > 1:408723/1‑143 (MQ=255)
cgtccGGCTCGCCCGGCGGCCTGTGTGTAAAGCTGAGCGGAACGCTTTGCCGAGCGAAACTCGGCGGAAAACAGCGCGCCGTCCACGTCGAGTAATGCTACCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTtg < 2:75174/140‑1 (MQ=255)
cccGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTtgtg < 1:600991/143‑1 (MQ=255)
aCGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGGAccga > 1:432156/1‑141 (MQ=255)
aCCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATcgg > 1:408716/1‑141 (MQ=255)
ccTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAGTCAAAATCcgc > 1:152233/1‑143 (MQ=255)
gcgtAACGCTGAGCGAAACGCCTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGcgcc < 1:210284/141‑1 (MQ=255)
gCTGAGCGATGCGCTTGGCCGGGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACAGCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGa < 2:95049/143‑1 (MQ=255)
gCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGa < 1:512392/143‑1 (MQ=255)
cTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGAt > 1:307678/1‑143 (MQ=255)
cGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTagt > 1:531824/1‑141 (MQ=255)
cGCTTTGCCGAGCGCACACCGGCAGCAACCAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTACTTCTGc < 2:576041/143‑1 (MQ=255)
|
GGCAAAGGCGTCGTAGCCTTTATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTACTTCTGC > NC_000913/4124957‑4125235
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
