Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F87 I1 R1
|
26 |
57.4 |
2238216 |
89.7% |
2007679 |
140.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,125,097 |
C→T |
E572K (GAG→AAG) |
priA ← |
primosome factor N' |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,125,097 | 0 | C | T | 97.9%
| 132.5
/ NA
| 49 | E572K (GAG→AAG) | priA | primosome factor N' |
| Reads supporting (aligned to +/- strand): ref base C (1/0); major base T (23/24); minor base G (0/1); total (24/25) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 |
CAAAGGCGTCGTAGCCTTTATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTACTTCT > NC_000913/4124959‑4125233
|
cAAAGGCGTCGTAGCCTTTATAGAGCAACGTTTGCAACAGAGGGTGGTCCGGATGTTGAGTTTGCAGCACCACTTCGCCCTGCTTACCCGCACGCCCGGCACGACCGGCGCCCGGAGTGTAAAGCAGAGCGAGACGCTCTGct > 2:626601/1‑142 (MQ=255)
gtcgtAGCCTTTTTAGAGCATCGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGaa < 2:89724/143‑1 (MQ=255)
ctgaGCCTCTATAGAGTTACGTTTGCAAGAGCGAACAATACGGATATTGCTTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGaaaa < 2:232058/140‑1 (MQ=255)
tAGCCTTTATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATc < 2:741169/143‑1 (MQ=255)
gCCTTTATAGGGCCACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCgg < 1:452907/143‑1 (MQ=255)
gCCTTTACAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCCCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGCAAATCgg < 1:430135/143‑1 (MQ=255)
gCCTGTATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCTTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCgg < 1:367690/143‑1 (MQ=255)
tttATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGc < 2:567134/126‑1 (MQ=255)
tttATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGc > 1:567134/1‑126 (MQ=255)
tatcGAGCAACGTTTGCAACAGAGGGCGTTCCGGATGGTGCGTTTGCAGCGCCACTTCGCCCTGTTTACCCGCACGCCCGTCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGaa < 2:466675/143‑1 (MQ=255)
taGAGCAACGTGTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGaaaa < 1:326203/143‑1 (MQ=255)
agCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAg < 1:187557/143‑1 (MQ=255)
gCAACGTTTGCAACAGTGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTGTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGTTTTGCCGAGCGAAAATCGGCAGAAAACAgc < 2:47352/143‑1 (MQ=255)
cAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGGCCGGCACGACCGGCGACCTGGGTGTAAAGCGGACCGAACCGCTTTGCCGAGCCAAAACACGCGGACAACAgcg > 1:1024863/1‑143 (MQ=255)
ttGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGACACGCTTTGCCGAGCGAAAGTCGGCAGAAAACAGCGCGCcgt > 1:464274/1‑143 (MQ=255)
aCAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGCCCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGCGCGAAAATCGGCGGAAAACAGTGCGCcgtccccg > 1:541699/1‑143 (MQ=255)
agagGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCAGGCACGACCGGCGCCCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAAGCGGCAGACAACAGCGCGCagtccaccgc > 2:821247/1‑140 (MQ=255)
ttCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGCGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATg > 1:637755/1‑143 (MQ=255)
gTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCCACCAGCg > 2:907928/1‑143 (MQ=255)
gCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCAGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGGCCACGCCCAGTAATGCAACCAGCggc > 2:451447/1‑141 (MQ=255)
tGCAGCACCTCTGCGCCCGGGTTAGCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTGTGCCGAGCGAAAAGCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATc < 2:315942/143‑1 (MQ=255)
gcagcaCCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATcc > 1:970470/1‑143 (MQ=255)
cagctCCACGTGGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTGAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCATGTCCAGTAATGCAACCAGCGTCACATCCg < 2:43886/143‑1 (MQ=255)
cagcaCCGCTCCGCCGTGTGTTCCCGCACGCCCGGCACGGCCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCg < 2:174917/143‑1 (MQ=255)
agcaCCATTTCGACCAGTTTACACGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGCGCCAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCgg < 1:869795/143‑1 (MQ=255)
agcaCCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAACCGCGTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCgg > 1:469330/1‑143 (MQ=255)
aaccccccTTCGCCCTGCTTACCCGCACCCCCGTCACGACCGGCGACCTGCGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCgg < 1:127072/136‑1 (MQ=255)
gtgtcccTGTTTTCTCGCACGCCCGGCACGACCGGCGAGCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCGGGAAATGGt < 2:794351/139‑1 (MQ=255)
cGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCGGGAAATGGTGa < 2:328789/143‑1 (MQ=255)
cGCCCTGGTGACGCGCGCTCCCGGCACGCCGGCCGACCCGGGGGGAAGGCAGGGCGAGACGCTTGGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGGCCACGTCCAGTAATGCAACCAGCGTCACATCCGGGAAATGGTGa < 2:309963/143‑1 (MQ=255)
cccTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCGGGACATAGTGAcc > 1:688404/1‑143 (MQ=255)
aCCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCa < 1:391535/143‑1 (MQ=255)
cccGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAg < 2:706706/143‑1 (MQ=255)
ccGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACCCTTTGCCGCGCGCAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCCACCAGCGTCACATCCGGGACATGGTGGCCTCTCGCCAGc > 1:971583/1‑143 (MQ=255)
cGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAAGCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCa < 2:272663/143‑1 (MQ=255)
gCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCCGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCag > 1:377661/1‑142 (MQ=255)
aCGCCCGGCAGCACCGGCGACCTGGGGGTAAAGCTGGGCGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCAttt < 1:349281/143‑1 (MQ=255)
ggCACGACCGGCGACCTGGGGGTAAAGCTGAGCGAGACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTAc < 2:666253/143‑1 (MQ=255)
ccGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCGGGAAATGGTGACCTTTCACCCACATATGTGTACCAAACaa > 2:71058/1‑143 (MQ=255)
gCGACCTGGGTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCGAGGAATGCAACCAGCGCCACATCCGGGAACTTGTGACCTCTCGCCAGCAGTTGCGCaccaaccacagt > 2:382852/1‑139 (MQ=255)
gggTGTAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTGGTGTACCAATCAACATCcgcgcg > 1:7692/1‑143 (MQ=255)
tAAAGCTGAGCGAAACGCTTTGCCGAGCGAAAATCAGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCGGGAAATGCTGACCTTTCGCCAGCATTTGAGTACCAATCAAAATCCGCGcgccgcc > 2:981085/1‑143 (MQ=255)
aGCTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCcgcg > 2:602408/1‑143 (MQ=255)
gccgAGCGAAACGCTTGGCCGAGCGAAAATCGGCAGAACACGGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGa < 2:556809/140‑1 (MQ=255)
cTGAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGAt > 1:458282/1‑143 (MQ=255)
gAGCGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCACAATCCGCGCGCCGCCGCCATGt > 1:768613/1‑143 (MQ=255)
cGAAACGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTACt > 2:969199/1‑143 (MQ=255)
aaaCGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTACTTc > 1:583473/1‑143 (MQ=255)
aaCGCTTTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACGTCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGCTGTAATTCt > 1:803060/1‑143 (MQ=255)
|
CAAAGGCGTCGTAGCCTTTATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCACATCCGGGAAATGGTGACCTTTCGCCAGCATTTGTGTACCAATCAAAATCCGCGCGCCGCCGCGATGTACTTCT > NC_000913/4124959‑4125233
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
