Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A29 F119 I0 R1	76	56.4	2152892	90.0%	1937602	142.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	459,834	C→A	100%	A316E (GCG→GAG)	lon →	Lon protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	459,834	0	C	A	100.0%	179.9 / ‑6.7	70	A316E (GCG→GAG)	lon	Lon protease
Reads supporting (aligned to +/- strand): ref base C (1/2); new base A (36/27); total (38/32)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGCGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAG  >  NC_000913/459694‑459968

gaAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCcgccgc                                                                                                                                      >  1:944591/1‑143 (MQ=255)

gaAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGgagc                                                                                                                                      >  1:258943/1‑143 (MQ=255)

           cAGAGTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGCGCAGGAAAGCCtt                                                                                                                           >  1:905943/1‑143 (MQ=255)

             gagTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGa                                                                                                                         <  1:974058/143‑1 (MQ=255)

             gagGTGCGGAAGCGGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTTGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGa                                                                                                                         <  1:355969/143‑1 (MQ=255)

                ttGCACATGCTGAAAATGATCTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATAc                                                                                                                      <  2:1019933/143‑1 (MQ=255)

                 ggtaGAAGGGGAAAATGATGGCTCCGATGGCGGCAGAAGCGACCGTAGTGCGTGGTTATGTCGACTGGAGGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATAcc                                                                                                                     <  1:336550/140‑1 (MQ=255)

                   cAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGa                                                                                                                   <  1:1073696/143‑1 (MQ=255)

                      aaGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGAGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATCCCGAGCa                                                                                                                >  2:1004453/1‑143 (MQ=255)

                          tGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTAt                                                                                                            >  2:909944/1‑143 (MQ=255)

                           gAAAATGATGTCTCCGATGTGGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATg                                                                                                           <  1:721301/143‑1 (MQ=255)

                           gAAAATGATGTCTCCGATGTCGGCAGCAGCGACCGTAGTACGTGGTTATATCGACTGGATGGTACCGGCGCTGTGGGAGGCGCGTAGCCAGGTCAAAGAAGACCTGCGGCCGGAGCCGGAAATCCTTGATACCGACCATtgtg                                                                                                           >  2:870715/1‑143 (MQ=255)

                                tgatgTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTg                                                                                                      <  1:128181/143‑1 (MQ=255)

                                tgatgGCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGGGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTg                                                                                                      <  2:203424/143‑1 (MQ=255)

                                  atgTCTCCGGTGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGa                                                                                                    <  1:591787/143‑1 (MQ=255)

                                  atgTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAACGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCGGGAGCAGGCAATCCGTGATAGCGGACAGTAGGGTCTGGa                                                                                                    >  2:186823/1‑143 (MQ=255)

                                  atgTCTCCGATGGCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGa                                                                                                    <  1:1511/143‑1 (MQ=255)

                                    gTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCACGAAATCCTTGATACCGACCATTATGGTCTGGAgc                                                                                                  >  1:384161/1‑143 (MQ=255)

                                     tctcCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAgcg                                                                                                 >  1:209812/1‑143 (MQ=255)

                                       tcCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGGCGCGGAAATAGTGTGGCAAGGTCAAAAGAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGGGcgcg                                                                                               >  1:149699/1‑143 (MQ=255)

                                        ccGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGt                                                                                              >  1:810318/1‑143 (MQ=255)

                                            tggcGGCATAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGGCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGaaa                                                                                          <  2:22700/140‑1 (MQ=255)

                                              tCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGa                                                                                        >  1:708494/1‑143 (MQ=255)

                                               cGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGGTGGTACAGGTGCCGTGGAATGCGCGGAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCGTTGATACCGACCATTATGGTCTAGAGCGCGTGaacgca                                                                                       >  2:964719/1‑139 (MQ=255)

                                                ggCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCCCGTGACAGCTc                                                                                      >  1:1058661/1‑143 (MQ=255)

                                                  cAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGa                                                                                    >  2:207935/1‑143 (MQ=255)

                                                   ataaGCGACCGTAGTGCCTTGTTATATCGACTGGATCGTACAGGTGCCGTGCAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGATCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGaa                                                                                   <  1:682987/141‑1 (MQ=255)

                                                       gCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCGGGTGGAGTAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCt                                                                               >  1:780374/1‑143 (MQ=255)

                                                        cGACCGGAGGGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCtt                                                                              <  1:1053844/143‑1 (MQ=255)

                                                            cGTAGTGCGTGGTTATATCGACTGGATTCGAGAGGTGCCGTGGAGTGCGCGTAGCAAGGGCGCACAAGACCTGCGTCAGTAGCAGGAAATCCTTGCTAGCGACGATGATGACCTGGCGCGCGTGAAAGATCGAATCCTTGact                                                                          >  2:428972/1‑141 (MQ=255)

                                                                 tGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCtt                                                                     >  1:838191/1‑143 (MQ=255)

                                                                   cGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGAACATTATGGTCTGGGGCGCGTGAAAGATCGAATCCTTGAGTATCTTGc                                                                   >  1:287656/1‑143 (MQ=255)

                                                                    gTGGTTATATCGACTGGATGGTACAGGTGCAGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAACGATCGAATCCTGGAGTATCTTGCg                                                                  >  2:816059/1‑143 (MQ=255)

                                                                       ggtataTCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGCGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGtt                                                               <  1:591670/141‑1 (MQ=255)

                                                                        ttATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGGCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGCGAAAGAGCGAATCCATGAGCATCTTGCggggc                                                              >  2:338264/1‑140 (MQ=255)

                                                                           tatCGNCTGGATGGTACAGGTGCCGTGGANTGCGCNTAGCAAGGTCAAANAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGNATCCTTGAGTATCTTGCGGTTCaaa                                                           <  1:623424/143‑1 (MQ=255)

                                                                             tCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGc                                                         >  1:73812/1‑143 (MQ=255)

                                                                             tCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGc                                                         >  1:658379/1‑143 (MQ=255)

                                                                                attgtgtGGGACAGTTGCCGTGGAGTGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCgt                                                      <  2:536497/137‑1 (MQ=255)

                                                                                aCTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCTGTTCGAAGCCgg                                                      >  2:570115/1‑142 (MQ=255)

                                                                                aCTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCgt                                                      >  1:731440/1‑143 (MQ=255)

                                                                                aCTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCgt                                                      <  1:19303/143‑1 (MQ=255)

                                                                                aCTGGATGGTACAGGGGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGCGCAGGAAATCCTTGCTACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCgt                                                      <  2:757284/143‑1 (MQ=255)

                                                                                    gATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTca                                                  <  1:187894/143‑1 (MQ=255)

                                                                                      tGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTcaac                                                <  1:1039172/143‑1 (MQ=255)

                                                                                        gTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTcaacaa                                              >  1:447486/1‑143 (MQ=255)

                                                                                        gTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTcaacaa                                              <  2:355536/143‑1 (MQ=255)

                                                                                         tACAGGTGCCGTGGAATGCGCGTATCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACaaa                                             <  2:852374/143‑1 (MQ=255)

                                                                                         tACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACaaa                                             >  1:473790/1‑143 (MQ=255)

                                                                                         accAGGTGCCGTGAAATGCGCGAAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACaaa                                             <  1:319158/141‑1 (MQ=255)

                                                                                              gTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCaa                                        >  1:489162/1‑143 (MQ=255)

                                                                                                 ccGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAggg                                     >  1:586532/1‑143 (MQ=255)

                                                                                                 ccGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCAGTGTCAACACAATAAAggg                                     >  1:117706/1‑143 (MQ=255)

                                                                                                      gAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAGCAAAATCAAGGGACCGa                                >  1:263446/1‑143 (MQ=255)

                                                                                                      aaaTGCGCGTAGCAAGGTCACAAAAGGCCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAATATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGa                                <  2:97108/142‑1 (MQ=255)

                                                                                                            gcgTAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGGGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCcact                          >  2:1004040/1‑143 (MQ=255)

                                                                                                            gcgTAGCAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCctct                          >  1:60437/1‑143 (MQ=255)

                                                                                                            gcgTAGCAAGGTCAAAAAAGACCTGCGTAAGGAGCAGGAAATCCTTGATCCCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGACTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGCACGAtcttct                          >  1:975840/1‑143 (MQ=255)

                                                                                                                 gCAAGGTCAAAACAGTCCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTg                     <  1:412924/143‑1 (MQ=255)

                                                                                                                  cAAGGTCAAAAAAGACCTGCGTCAGGGGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTgg                    <  2:209812/143‑1 (MQ=255)

                                                                                                                  cAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGACAGATCGAATCCTTGAGTATCTTCCGGTTCCCAGCCGTGTCAACAAAACCAAGGGACCGATCCTCTGCCTgg                    >  1:263410/1‑143 (MQ=255)

                                                                                                                  cAAGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCCgg                    >  2:98911/1‑143 (MQ=255)

                                                                                                                   taGGTCAAAAAAGACCTGCGTCAGGAGCAGGAAACCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGt                   <  2:545919/142‑1 (MQ=255)

                                                                                                                      gTCAGAAAAGACCTGCGGCAGGGGCAGGCAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAgg                <  2:200184/143‑1 (MQ=255)

                                                                                                                      gTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAgg                >  2:598160/1‑143 (MQ=255)

                                                                                                                      gTCAAAAAAGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAgg                >  2:1019992/1‑143 (MQ=255)

                                                                                                                       tCAAACACGGCCGGCGTCAGAGGCAGGAAATCCTTGATACCGACCATGATGGTCTGGAGCGCGTGAAAGATTGAAGCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAggg               <  2:643217/143‑1 (MQ=255)

                                                                                                                         aaaaaaGACCTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGAAGCGCGTGAAAGCTCGAATCCTTGAGTATCTTGCGGTTCAAAGAGGTGTCAACAAAATCAACGGACAGATCCTCTGCCGGGTCGGgcc             >  2:487489/1‑143 (MQ=255)

                                                                                                                          aaaagGACCTGCGTCAGGAGCAGGAAATCCTGTATACCGACCATGATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGgccg            <  2:37249/143‑1 (MQ=255)

                                                                                                                                 ccTGCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCggggg     <  1:570115/143‑1 (MQ=255)

                                                                                                                                    gggTCGGGAGCAGCAAATCGTTGATGCCTACTAGGATTGTCTGGAGCGCGGGAAGGAGCGAATCCCTGGGTGTCTGGCGGTGCAAAGCCGTGTCAGCAAAATCAGGGGACCGGTCCTCTGCCTGGTAGGGCCGCCGGGggtag  <  2:1064230/141‑1 (MQ=255)

                                                                                                                                    gCGTCAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATGCTCTGCCTGGCAGGGGCGCCGCGgggag  >  2:550013/1‑143 (MQ=255)

GAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGCGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAG  >  NC_000913/459694‑459968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A