Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A29 F71 I1 R1	22	78.5	2942462	91.4%	2689410	142.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	459,834	C→A	A316E (GCG→GAG)	lon →	Lon protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	459,834	0	C	A	98.5%	185.0 / NA	70	A316E (GCG→GAG)	lon	Lon protease
Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (33/34); total (36/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGCGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTA  >  NC_000913/459701‑459971

gAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAAt                                                                                                                                  >  1:890125/1‑143 (MQ=255)

    cAGCGTTGCAGAAGCTGAAACTGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCtt                                                                                                                              <  1:121934/143‑1 (MQ=255)

     agagTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTg                                                                                                                             <  2:55971/143‑1 (MQ=255)

      gagTTTCAGAAGCTGAAAAGGATGTCTCCGATGTCGGAAGAAGCGACCGTAGGGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGa                                                                                                                            <  1:1284684/143‑1 (MQ=255)

       agTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTGGTGCGTGGTTATATCGACTGGATGGGACAGGTGCCGTGGAATGCGCGTAGCAAGGGCAAAAAAGACCTGCGT‑CAGGAGCCGGCAAGCCTTGAt                                                                                                                           >  1:96019/1‑143 (MQ=255)

         ttGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATAc                                                                                                                         >  2:545462/1‑143 (MQ=255)

            cAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGa                                                                                                                      <  1:631935/143‑1 (MQ=255)

             aGAAGCCGAAAATGATGTCCCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGAc                                                                                                                     <  1:556922/143‑1 (MQ=255)

                   tGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTAt                                                                                                               >  1:652139/1‑143 (MQ=255)

                    gAAGATGCTGTCTGCGATGTCGGCTGAAGGGACGGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATg                                                                                                              <  2:821165/143‑1 (MQ=255)

                    gAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTATAGGTGAAGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGAACATCCTTGATACCGACCATTATg                                                                                                              >  2:174678/1‑143 (MQ=255)

                            tgTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGGCTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGAGCATTATGGTCTGGAg                                                                                                      >  1:1029908/1‑143 (MQ=255)

                             gTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAACGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATCGTCTGGAgc                                                                                                     >  2:246416/1‑143 (MQ=255)

                              tctcCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGGGGAATGCGCGTAGCAAGGTCGAAAACGACCTGCGC‑CAGGAGCAGGAAATCCTTGATACCGGCCATTAGGGTCggggggg                                                                                                    >  1:880016/1‑139 (MQ=255)

                              tctcCGACGTCCGCAGAAGCGACCGTAGTGCGCGGTTATATCGACTTGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAgcg                                                                                                    <  1:1468444/143‑1 (MQ=255)

                                tctgATGTCGGCAGAAGCGACCGGAGTGCGTGGTTGTATCGACTGGATGGTACAGGTGCCGGGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGcgcg                                                                                                  <  2:1253854/140‑1 (MQ=255)

                                tcCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGGGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGGGCAGGAAATCCTTGATACCGCCCACTATGGTCCGGAGcgcg                                                                                                  >  2:716294/1‑143 (MQ=255)

                                 ccGGTGTCGGCAGAAGCGACCGTAGTGCGTGGTGAGAGCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGt                                                                                                 <  2:343933/143‑1 (MQ=255)

                                  cGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCTGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTg                                                                                                >  2:776129/1‑143 (MQ=255)

                                    aTGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGaa                                                                                              >  1:834161/1‑143 (MQ=255)

                                     tGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGaaa                                                                                             <  2:216751/143‑1 (MQ=255)

                                       tCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATACTTGATACCGACCATCATGGTCTGGAGCGCGTGAAAGa                                                                                           >  2:853810/1‑143 (MQ=255)

                                        cGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGAt                                                                                          <  2:890125/143‑1 (MQ=255)

                                         tgaaGACGCGACTGTAATGAGTGGTTATATTGACTGGATGCTACAGGTGCCGTGGAATGCGCGTAGCAAGAGCAAAGAATACTTGACT‑TGGCAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATc                                                                                         <  2:518552/140‑1 (MQ=255)

                                         tccAGAAGCGCCCGTAGTGCGTGGTTATATCGACTGGCTGGCGCAGGTGCCGTGGAATTCCTTCATCTTAGTCAACAACGACCCGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATc                                                                                         <  1:1458548/141‑1 (MQ=255)

                                         ggaggAAGCGATCGTGGTGCGTGGTTATATCGAGAGAATGGTAGAGGTGGCGTGGAATGCGCGTAGCAAGGTCAAAAAAGATCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATc                                                                                         <  2:884242/139‑1 (MQ=255)

                                         ggCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTGCAGG‑GCGTAAAATCCTTGATACCGACCGTTATGGTCTGGCGCGCGTGAAAGATc                                                                                         >  2:1039022/1‑143 (MQ=255)

                                         ggCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATc                                                                                         <  2:457670/143‑1 (MQ=255)

                                          gCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCg                                                                                        >  2:1200143/1‑143 (MQ=255)

                                                gCGACCGTAGTGCGTGGGTATATCGACGGGATGGTACAGGTGCCGTGGAATGCGCGGGGCAAGGGCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGCGAAAGATCGAATCCt                                                                                  >  1:1197962/1‑143 (MQ=255)

                                                   tgcggaGTGCGTGGTTGTATCGACTGGGTGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGGCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGGGTGGAGCGCGTGAAAGATCGAATCCTTGa                                                                               <  2:681652/138‑1 (MQ=255)

                                                   gaggtggTGCGTGGTTATATCGACTGGAGGGTACAGGTGCCTGGGAATGCGCGTAGCAAGGTCATAAAAGCCCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGa                                                                               <  2:173630/137‑1 (MQ=255)

                                                   aCCGTAGTGGGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGa                                                                               <  2:32297/143‑1 (MQ=255)

                                                       tAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCGTGCGTAt                                                                           >  1:197245/1‑143 (MQ=255)

                                                          tGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGGCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGGTCGAACGCTCGAGTATCtt                                                                        >  2:198061/1‑143 (MQ=255)

                                                           gggtagTTATCTCGACTGAATAGTACAGGTGCCATGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTg                                                                       <  2:660243/138‑1 (MQ=255)

                                                            cGTGGTTATATCGACTGGAGGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CGGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGc                                                                      <  2:608045/143‑1 (MQ=255)

                                                               ggTCATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGt                                                                   >  2:815509/1‑143 (MQ=255)

                                                                 tgatgtCGACTTGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTc                                                                 <  1:1640/138‑1 (MQ=255)

                                                                  tataTCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAGGATCGAATCCTTGAGTATCTTGCGGGTCa                                                                >  2:403857/1‑143 (MQ=255)

                                                                    tatCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAATGACCTGCGT‑CAGGAGCAGGAATTTCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGACTCCTTGAGTATCTTGCGGTTCaaa                                                              <  1:362217/143‑1 (MQ=255)

                                                                     atCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGGCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTGTGGGCTGGAGCGCGTGATAGATCGGAGCCTTGGGTATTTTGCGGTTCGAAg                                                             >  1:496363/1‑143 (MQ=255)

                                                                      tCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCCAGGTCAAAAAAGACCTGCGT‑CAGGCGCAGGCAATCCTTGATACCGACCATTATGGACTGGAGCGCGTGAAAGATCAAATCCTTGAGTCACTTGCGGGTCAAAGc                                                            >  2:1152721/1‑143 (MQ=255)

                                                                      tCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGc                                                            >  1:1469902/1‑143 (MQ=255)

                                                                         aCTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCgt                                                         >  1:235731/1‑143 (MQ=255)

                                                                           gggAGGGTACAGGTGCCGTGGAATGCGCGTAGCGAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCgtgt                                                       <  2:516090/142‑1 (MQ=255)

                                                                              aTGGTACAGGTGCGGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTcaa                                                    <  2:1443204/143‑1 (MQ=255)

                                                                              aTGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACTTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTcaa                                                    >  2:102015/1‑143 (MQ=255)

                                                                              aTGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGACTCCTTGAGTATCTTGCGGTTCAAAGCCGTGTcaa                                                    >  2:783636/1‑143 (MQ=255)

                                                                              aTGGTAACGGTGGCGTGGAATGCGAGCAGCAAGGTCGAAAAAGCCCTGCGT‑CAGGACGAGGAAATCCTAGAGACCGACGATGACGGTCTGGGGCGCGTGAAAGCTCGAATCCGTGAGTATCTTGCGGTTCAAAGCCGTGTcaa                                                    <  2:511419/143‑1 (MQ=255)

                                                                                   aCAGGTGCCGTGGAATGCGCGTAGCAAGGACAAAACAGGCCTGCGT‑GAGGAGCAGGAAATCCTTGCTATGAGGTATTATGGTCTGGAGCGCGGGAAGGATCGAGTGCTTGAGTATCTTGCGGTTCAAGGCGGTGTCAACaaaa                                               >  2:1042569/1‑143 (MQ=255)

                                                                                    cAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAAt                                              <  1:1125960/143‑1 (MQ=255)

                                                                                     aGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGGTCAAAGCCGTGTCAACAAAATc                                             >  1:1026331/1‑143 (MQ=255)

                                                                                       gTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTCTGTTGCGGTTCAAAGCCGTGTCAACAAAATcga                                           >  1:392660/1‑141 (MQ=255)

                                                                                       gTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCaa                                           <  1:1040932/143‑1 (MQ=255)

                                                                                       gTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGACCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCaa                                           <  1:9800/143‑1 (MQ=255)

                                                                                        tGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGCCAACAAAATCaac                                          >  2:327853/1‑142 (MQ=255)

                                                                                            gTGGAATGCGCGTAGCAAGGTCAAAAAGGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTc                                                                 <  2:1130185/116‑1 (MQ=255)

                                                                                            gTGGAATGCGCGTAGCAAGGTCAAAAAGGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTc                                                                 >  1:1130185/1‑116 (MQ=255)

                                                                                                           caggctCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTCGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTgg                       <  2:1267882/138‑1 (MQ=255)

                                                                                                           cAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTgg                       >  1:1048248/1‑143 (MQ=255)

                                                                                                           cAAGGCCAAAAACGACCTGCGT‑CAGGAGCAGAAAATCCTTGATACCGACCATTATGGGCTGGGGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTGCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTgg                       <  2:1109901/143‑1 (MQ=255)

                                                                                                             agctCAAACAAGCCCCGCGG‑CAGGAGCAGGAAATCCTTGCTACCGACCATTATGGTCTGGACCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTa                     <  1:1364808/140‑1 (MQ=255)

                                                                                                             aGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGTACCGATCCTCTGTCTGGtc                     >  1:517286/1‑142 (MQ=255)

                                                                                                                ccAAAAAAGCTCTGCGT‑CAGGATCAGGAAATCGTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAggg                  <  2:1125938/142‑1 (MQ=255)

                                                                                                                      aaGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATAATGGGGTGGAGCGCGTGAGAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCGAGGGACCGATCGTCTGCCTGGTAGGGccgccg            >  1:775764/1‑143 (MQ=255)

                                                                                                                       aGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTATGGAGCGCGTGAAAGATCGAATCCTAGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCgg           >  1:1029091/1‑143 (MQ=255)

                                                                                                                               gT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGggtaggt   <  1:90340/143‑1 (MQ=255)

                                                                                                                                t‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGTCGCGCTTGAAAGATCGAATCCTCGAGTCTCTTGCGGTTCAAAGCCGTGTCAACAAAATCCAGGGCCCGATCCTCTGCCTGGAAGGGCCGCCGGGggtaggtg  >  1:1007039/1‑142 (MQ=255)

                                                                                                                                t‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGggtaggta  >  1:925139/1‑143 (MQ=255)

GAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGCGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTA  >  NC_000913/459701‑459971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A