Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A27 F124 I0 R1	44	29.6	1159012	90.6%	1050064	142.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	802,778	G→T	48.4%	V298L (GTG→TTG)	ybhI →	putative tricarboxylate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	802,778	0	G	T	48.4%	‑2.3 / 23.1	21	V298L (GTG→TTG)	ybhI	putative tricarboxylate transporter
Reads supporting (aligned to +/- strand): ref base G (6/5); new base T (5/5); total (11/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.26e-01

GTGGATAACAAAACCATCGCTAAAGCGGGCCTTGCCGAACTAGGACCGATGAAAATCCGCGAAAAAATGCTGCTCGGTGTCTTTGTGCTGGCGCTGCTGGGCTGGATTTTCAGTAAGTCTCTGGGGGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGTATCGTTACCTGGGAAGACGTGGTTAAAAATAAAGGCGGCTGGAATACCTTAATCTGGTACGGCGGTATTATCGGCTTAAGCTCCTTATT  >  NC_000913/802637‑802905

gTGGATAACAAAACCATCGCGAAAGCGGGCCTTGCCGAACTAGGACCGATGAAAATCCGCGAAAAAATGCTGCTCGGTGTCTTTGTGCTGGCGCTGCTGGGCTGGATTTTCAGTAAGTCTCTGGGGGTTGATGAATCCACCGt                                                                                                                                <  1:270230/143‑1 (MQ=255)

          aaaCCATCGCTAAAGCGGGCCTTGCCGAACTAGGACCGATGAAAATCCGCGAAAAAATGCTGCTCGGTGTCTTTGTGCTGGCGCTGCTGGGCTGGATTTTCAGTAAGTCTCTGGGGGTTGATGAATCCACCTTGGCAATCgtt                                                                                                                      >  2:539495/1‑143 (MQ=255)

          aaaCCATCGCTAAAGCGGGCCTTGCCGAACTAGGACCGATGAAAATCCGCGAAAAAATGCTGCTCGGTGTCTTTGTGCTGGCGCTGCTGGGCTGGATTTGCAGTAAGTCTCTGGGGGTTGATGAATCCACCGTGGCAATCgtt                                                                                                                      <  1:536334/143‑1 (MQ=255)

            aCCATCGCTAAAGCGGGCCTTGCCGAACTAGGACCGATGAAAATCCGCGAAAAAATGCTGCTCGGTGTCTTTGTGCTGGCGCTGCTGGGCTGGATTTTCAGTAAGTCTCTGGGGGTTGATGAATCCACCTTGGCAATCgttgt                                                                                                                    <  2:91952/143‑1 (MQ=255)

                                                          gcgAGAAGATGCTGCTCGGTGGCTTTGTGCTGGCGCTGCTGGGCTGGATTTTCAGTAAGTCTCTGGGGGTTGATGAATCCACCTTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGTATCGTTACCTGGGAAGACGTg                                                                      <  2:203613/143‑1 (MQ=255)

                                                            gAAAAAATGCTGCTCGGTGTCTTTGTGCTGGCGCTGCTGGGCTGGATTTTCAGTAAGTCTCTGGGGGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGTATCGTTACCTGGGAAGACGTGGt                                                                    >  1:427826/1‑143 (MQ=255)

                                                                 gaTGCTGCTCGGTGTCTTTGTGCTGGCGCTGCTGGGCTGGATTTTCAGTAAGTCTCTGGGGGTTGATGAATCCACCTTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGTATCGTTACCTGGGAAGACGTGGTTaaaa                                                               <  2:191628/142‑1 (MQ=255)

                                                                    gctgctCGGTGTCTTTGTGCTGGCGCTGCTGGGCTGGATTTTCAGTAAGTCTCTGGGGGTTGATGAATCCACCTTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGTATCGTTACCTGGGAAGACGTGGTTAAAAATa                                                            <  1:539495/143‑1 (MQ=255)

                                                                       gctCGGTGTCTTTGTGCTGGCGCTGCTGGGCTGGATTTTCAGTAAGTGTCTGGGGGTTGGTGAATCCAGCGTGTCAATCGTTGTTATTGCAACCATGCTGCGGCTTGGTATCGTTAACGGGGAAGACGACGTTAAAAATGAAg                                                         >  2:464908/1‑143 (MQ=255)

                                                                        ctCGGTGTCTTTGTGCTGGCGCTCCTGGGCTGGTGGTTCAGTAAGTCTCTGGGGGTTGATGAATCCACCTTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGTATCGTTACCTGGGAAGACGTGGTTAAAAATAAAgg                                                        >  1:271684/1‑143 (MQ=255)

                                                                                 tttGTGCTGGCGCTGCTGGGCTGGATTTTCAGTAAGTCTCTGGGGGTTGATGAATCCACCTTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGTATCGTTACCTGGGAAGCCGTGGTTAAAAATAAAGGCCGCGGGag                                               >  1:293928/1‑142 (MQ=255)

                                                                                        tGGCGCTGCTGGTGGGGATGTTCAGTAGGTCTTTGGGTGTTGAGGAATCCACCGGGGCCATCGCTGGTATGGCAACCATGCTGCTGCTGGGTATCGTTACCTGGGAAGACGTGGTTAAAAATAAAGGCGGCTGGAATACCTTa                                        <  2:143626/143‑1 (MQ=255)

                                                                                          gggcggctgggcttgAGTGCTAGGAAGGCTCGGGGGGTGGATTAATCCAACGTGGCAATCGGTGATATGGCGACCATGCTGCTGCTGGGGATCGTTACCTGGGAAGACGGGGTTAAAAATAAAGGCGGCTGGAATACCTTAAt                                      <  2:316095/138‑1 (MQ=255)

                                                                                             ctgcggGGCGGGATTTTCAGTTAGTCTAGGGGGGTTGATGAATCCACCTTGGGAATCGTTGTTATGGCAAGCATGCTGCTGCTGGGTATCGTTACCTGGGAAGACGTGGTTAAAAATAAAGGCGGCTGGAATACCTTAATCTg                                   <  2:41596/143‑1 (MQ=255)

                                                                                                          ttttCAGTAAGTCTCTGGGGGTTGATGAATCCACCTTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGTATCGTTACCTGGGAAGACGTGGTTAAAAATAAAGGCGGCTGGAATACCTTAATCTGGTACGGCGGtatt                      >  1:494363/1‑143 (MQ=255)

                                                                                                          ttttCAGTAAGTCTCTGGGGGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGTATCGTTACCTGGGAAGACGTGGTTAAAAATAAAGGCGGCTGGAATACCTTAATATGGTACGGCGGtatt                      >  1:425434/1‑143 (MQ=255)

                                                                                                           tttCAGTAAGTCTCTGGGGGTTGATGAATCCACCTTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGTATCGTTACCTGGGAAGACGTGGTTAAAAATAAAGGCGGCTGGAATACCTTAATCTGGTACGGCGGtatta                     >  1:526198/1‑143 (MQ=255)

                                                                                                                gTAAGTCTCTGGGGGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGTATCGTTACCTGGGAAGACGTGGTTAAAAATAAAGGCGGCTGGAATACCTTAATCTGGTACGGCGGTATTATCGGc                >  1:221034/1‑143 (MQ=255)

                                                                                                                  aaGTCTCAGGGGGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGTATCGTTAGCTGGGAAGACGTGGTTAAAAATAAAGGCGGCTGGAATACCTTAATCTGGTACGGCGGTATTATCGGCtt              <  2:19076/143‑1 (MQ=255)

                                                                                                                          gggggTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGTATCGTTACCTGGGAAGACGTGGTTAAAAATAAAGGCGGCTGGAATAACTTAATCTGGTACGGCGGTATTGTCTGCTTAAGCTCCt      >  1:256803/1‑143 (MQ=255)

                                                                                                                              gTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGTATCGTTACCTGGGAAGACGTGGTTAAAAATAAAGGCGGCTGGAATACCTTAATCTTGTACGGCGGTATTATCGGCTTAAGCTCCtgatt  >  1:472780/1‑143 (MQ=255)

GTGGATAACAAAACCATCGCTAAAGCGGGCCTTGCCGAACTAGGACCGATGAAAATCCGCGAAAAAATGCTGCTCGGTGTCTTTGTGCTGGCGCTGCTGGGCTGGATTTTCAGTAAGTCTCTGGGGGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGTATCGTTACCTGGGAAGACGTGGTTAAAAATAAAGGCGGCTGGAATACCTTAATCTGGTACGGCGGTATTATCGGCTTAAGCTCCTTATT  >  NC_000913/802637‑802905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A