Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A9 F77 I0 R1
|
41 |
21.1 |
946138 |
88.6% |
838278 |
142.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
710,595 |
G→T |
100% |
P18T (CCT→ACT) |
fur ← |
DNA‑binding transcriptional dual regulator Fur |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 710,595 | 0 | G | T | 100.0%
| 57.6
/ NA
| 21 | P18T (CCT→ACT) | fur | DNA‑binding transcriptional dual regulator Fur |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (15/6); total (15/6) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTA > NC_000913/710469‑710727
|
tCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGCCGTGATGGTTGTCCGGCTCGTGAAGAACTTCCAGGTTTTTTAAACGAGTAAGCGTTTCTTTTAgg > 1:165074/1‑143 (MQ=255)
tACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGGTCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTAGTTTGCGGCCAg > 1:203130/1‑143 (MQ=255)
aCGCGATATACCGTAGCCAGACCAATTTCTTCACCCGTATCTATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGc < 2:79509/143‑1 (MQ=255)
cgcgATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGct > 1:43814/1‑143 (MQ=255)
aGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTGTCTTTAGGGCGGTAtt > 1:72453/1‑143 (MQ=255)
cAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTCGGGCGGTATTGTTa > 1:329728/1‑143 (MQ=255)
ttcACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATATTTATCAGTCa > 1:297314/1‑143 (MQ=255)
aCCCATATCGGTCTGTCTTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGACCTTCCAGGATTTTTAATCTAGTAAGCTTTACTTTCAGGCCAGCTTTCTTTAGGGTGGTATTGTTATCAGTCATGc < 2:93981/143‑1 (MQ=255)
aTCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTCTAGGGCGGCATCGTTCTCAGTCATGCGGCATCCGTc > 2:75204/1‑143 (MQ=255)
tCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTcc > 1:439536/1‑143 (MQ=255)
aaaTCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTACGGGGt > 1:212197/1‑143 (MQ=255)
aaaTCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGt > 1:449090/1‑143 (MQ=255)
tGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGa < 2:43814/143‑1 (MQ=255)
gCTCCTGAAGAACTTCCAGCAATTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTTTTTAGGGCGGAATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGCGAAAGAATTTGc > 2:320108/1‑143 (MQ=255)
tCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGctc > 1:158196/1‑143 (MQ=255)
tCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATGTGctc > 1:158198/1‑143 (MQ=255)
tGAAGAACTTCCAGGATCTTTAAACGAGTAAGCGTTACTTTCATGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTg < 2:354648/143‑1 (MQ=255)
gaagaaCTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTAGTGTTATCAGTCATGCGGAATCTGTCCTGTTAATAAGCGGTTCACTTCATTGGAAGAAGTGACAGAATTTGCTCCTga > 2:60887/1‑143 (MQ=255)
gaagaaCTTCCAGGATTGTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTga < 2:174030/143‑1 (MQ=255)
ttCCAGGATTTTTAACCGACTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATg < 2:329728/143‑1 (MQ=255)
tttAAACGAGTAAGCGTTACTTTCATGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCCGTCCTGTTACTAAGTGGTTCACCTCATTAGAACAAGTGAAAGAATTTGCTGTTGAGATAATGCTTATCATta > 2:54193/1‑143 (MQ=255)
|
TCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTA > NC_000913/710469‑710727
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
