Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A19 F50 I1 R1 21 62.9 2410612 90.0% 2169550 142.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 710,595 G→T P18T (CCT→ACT)  fur ← DNA‑binding transcriptional dual regulator Fur

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913710,5950GT98.2% 165.5 / NA 57P18T (CCT→ACT) furDNA‑binding transcriptional dual regulator Fur
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (29/26);  minor base C (0/1);  total (30/27)
Fisher's exact test for biased strand distribution p-value = 4.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCGTCAAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATT  >  NC_000913/710455‑710734
                                                                                                                                            |                                                                                                                                           
gtcgtcAGACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTaa                                                                                                                                           <  2:656037/143‑1 (MQ=255)
gacggcAAACTGGTTCAGTTCGCGATATACCGTAGCCAGACCAATTTCTTCCCCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTaa                                                                                                                                           <  2:357841/138‑1 (MQ=255)
      aaaCTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGAATTTTAAACGAGAAAGCGTTa                                                                                                                                     >  2:494533/1‑143 (MQ=255)
            gTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCa                                                                                                                               <  1:790049/143‑1 (MQ=255)
                 ggaCGCTATATACCTTGGCTAGACCAATTTCGCCATGCATAGTGATCAGACGTTTGTATAAATCTTCCGTACTGACGTGATGGTTGGCCGGCTCCTGAAGAACTTCCAGGATTTTTAATCGTGTAAGCGTTACTTTCAGGCCa                                                                                                                          <  2:506319/141‑1 (MQ=255)
                  tACGCGTTATCCCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGTCGTTTGTATAAATCTTCCGCGCTGTCGTTATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTGTCAGGCCAg                                                                                                                         <  2:915729/143‑1 (MQ=255)
                   aCGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGc                                                                                                                        >  2:1191239/1‑143 (MQ=255)
                          ataCCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGctttcttt                                                                                                                 <  2:36254/143‑1 (MQ=255)
                                     aGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTAtt                                                                                                      >  2:466228/1‑143 (MQ=255)
                                     aGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTAAGGCCAGCTTTCTTTAGGGCgggagt                                                                                                      >  2:388068/1‑139 (MQ=255)
                                        ccAATTTCTTCACCCACATCGATCAGACGTTTGTATAAATCTTCCGCACTTACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGtt                                                                                                   <  1:44630/143‑1 (MQ=255)
                                         cAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTa                                                                                                  >  2:960083/1‑143 (MQ=255)
                                         cAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTACGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTATAGGGCGGTATTGTTa                                                                                                  >  2:613330/1‑143 (MQ=255)
                                           aTTTCTTCACCCATATCGATCAGCCGCTTTCATAAATCTTCCGCACTGACGTGTTGGATGCCCACCCACTGGCGAACTCCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTCGGGCGGTACTGTtctc                                                                                                >  2:252876/1‑143 (MQ=255)
                                           aTTTCTGCACCCATCTCGATCATACGTGTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATc                                                                                                <  2:255195/143‑1 (MQ=255)
                                            tttCTTCACCCATATCGATCATACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCa                                                                                               <  1:133892/143‑1 (MQ=255)
                                            tttCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCa                                                                                               <  1:544596/143‑1 (MQ=255)
                                               cttcACCCATATCGATCAGACGTTTGTATAAATCTCCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTc                                                                                            <  1:418031/143‑1 (MQ=255)
                                                ttcACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTACGGCTCCTGAAGAACTGCCAGGATTTTTAAACGCGTCAGCGTTACTTTCAGGCCAACTTTCTGTAGGGCGGTGTTGTTATGAGtaa                                                                                           >  2:675643/1‑141 (MQ=255)
                                                   aCCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGc                                                                                        >  1:292942/1‑143 (MQ=255)
                                                    cccATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTTAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCg                                                                                       <  2:529504/143‑1 (MQ=255)
                                                    cccATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCg                                                                                       >  2:949070/1‑143 (MQ=255)
                                                     ccATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGCTACTTTAAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCgg                                                                                      >  1:268649/1‑143 (MQ=255)
                                                             aTCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTGTGATAATCCAGCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTc                                                                              >  1:875582/1‑143 (MQ=255)
                                                             aTCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTc                                                                              >  1:97447/1‑143 (MQ=255)
                                                                aGACGTTTGTATAAATCCTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGCTTTTTAAACGGGTCAGCGATACTTTCAGGCCAGCTTACTTTAGGGCGGTATTGTTATCAGCCATGCCGAATCTTTCCTg                                                                           >  2:975519/1‑143 (MQ=255)
                                                                   cGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTa                                                                        <  1:946241/143‑1 (MQ=255)
                                                                   cGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTa                                                                        <  1:31957/143‑1 (MQ=255)
                                                                   cGTGTGTATAGATCTTGCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTa                                                                        <  1:69247/143‑1 (MQ=255)
                                                                        gTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGCAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAg                                                                   <  1:981322/143‑1 (MQ=255)
                                                                          agaaaTCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCg                                                                 <  1:413716/141‑1 (MQ=255)
                                                                           taAATCTTCCGCACTGGCTTGATGGTTGTCCGGCTGCTGAAGAACTTCCAGGATTTTTAAACGTGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATTAATCATGCTGATTCTGTCCTGTTACTAAGCgg                                                                <  2:147163/143‑1 (MQ=255)
                                                                             aaTCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGtt                                                              <  1:250493/143‑1 (MQ=255)
                                                                              aTCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTGTCAGGCATGCGGAATCTGTCCTGTTACTAAGCGGtta                                                             >  1:660486/1‑142 (MQ=255)
                                                                                 ttCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTCACTAAGCGGTTCACt                                                          >  2:596600/1‑143 (MQ=255)
                                                                                    cgaaCTGACGTGATGGTGGTCCGGCGCCTGAAGAACTTCCAGGAGTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCa                                                       <  1:147570/140‑1 (MQ=255)
                                                                                     gCAGTGACGGTATGGGTGTCCGGCTCCTGAAGAACTGCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCAt                                                      <  2:630217/143‑1 (MQ=255)
                                                                                     gCACTGACGTTATGGTTGTTCGGCTTCTGTATAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTCATCATGCATCCGGACACCGTCCTGGTTCTAATCGGTTCACTTCag                                                      >  2:800754/1‑142 (MQ=255)
                                                                                          gACGTTATGGTTGTCCGGCTCCTGAAGATCTTCCAGCATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTagaa                                                 <  2:458391/143‑1 (MQ=255)
                                                                                           aCGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTagaag                                                >  1:1104441/1‑143 (MQ=255)
                                                                                                 tGGTTGTCCGGCTCCTGAAGAACGTGCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGa                                          <  2:339433/143‑1 (MQ=255)
                                                                                                     tGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGACACGAGCGGTCTGTCCTGTTACTAAGGGGTTCACTTCATTAGAAGCAGTGCCAGa                                      >  1:873247/1‑143 (MQ=255)
                                                                                                        ccGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAAtt                                   >  1:1125964/1‑143 (MQ=255)
                                                                                                           gCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTAAGGCCAGCTTTCATTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACCTCCTTAGAAGAAGTGGCAGAATTTGc                                >  1:1097747/1‑143 (MQ=255)
                                                                                                                tGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATATGTCATGATACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTg                           >  1:551007/1‑143 (MQ=255)
                                                                                                                   agaaCTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTgaga                        >  1:782358/1‑143 (MQ=255)
                                                                                                                         tCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACGTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGc                  >  1:1814/1‑143 (MQ=255)
                                                                                                                               ctttttAAACGCGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGTTCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCa            <  1:220093/142‑1 (MQ=255)
                                                                                                                               aTTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGGATCTGTCCTGTTACTAAGCGGTTCACTTCATTCGAAGAAGTGACAGAATTGGCTCTTGAGATAATGCTTTTCa            >  1:249035/1‑143 (MQ=255)
                                                                                                                                 ttttAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGGCCTGTTCCCAAGCGGTTCACTTCATTAGCAGACGTGATGGTTTTTGCTCTCGAGATTATTCGTGTCAtt          >  1:812695/1‑143 (MQ=255)
                                                                                                                                   ttAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATCGTCATCAGGCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATtat        <  1:1191239/143‑1 (MQ=255)
                                                                                                                                    tAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATtata       <  1:949070/143‑1 (MQ=255)
                                                                                                                                     aaaCGGGTAAGCGATACTTTCAGGCCAGCTTTCTTTAGGGAGGTATTGTTATCAGTCATGCGGAATCTGTCCTATTACTAAGCTGTTAACTGCATTAGAAGAAGTGACAGAATTTGCATATGAGATAATGCGTATCATTATAg      >  2:69093/1‑143 (MQ=255)
                                                                                                                                      aaCGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGACGTGCCAGAATGTGCTCTTGAGATCATGCGTATCATTATAGa     >  2:644546/1‑143 (MQ=255)
                                                                                                                                      aaCGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGa     >  1:97324/1‑143 (MQ=255)
                                                                                                                                      aaCGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGGCCTGGTACTAAGCGGTTCACGTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGa     <  1:390944/143‑1 (MQ=255)
                                                                                                                                         gAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTGTCATTATAGAAtt  >  1:776965/1‑143 (MQ=255)
                                                                                                                                            |                                                                                                                                           
GTCGTCAAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATT  >  NC_000913/710455‑710734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A