Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A17 F104 I1 R1
|
25 |
31.6 |
1325236 |
90.2% |
1195362 |
142.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,196,196 |
T→C |
intergenic (‑97/+136) |
thiC ← / ← rsd |
phosphomethylpyrimidine synthase/regulator of sigma D |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,196,196 | 0 | T | C | 100.0%
| 68.7
/ NA
| 24 | intergenic (‑97/+136) | thiC/rsd | phosphomethylpyrimidine synthase/regulator of sigma D |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (9/15); total (9/15) |
CCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCA > NC_000913/4196057‑4196327
|
cccGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAGAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTcc > 1:343267/1‑143 (MQ=255)
gcgtGTTGTTCGCGGCGGGTCAGTTTTGGTGCCGACATAGCTCAGTCCAAAAAGTTAACGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCg < 2:25694/143‑1 (MQ=255)
cAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAgg > 1:105996/1‑143 (MQ=255)
cAGTTTGGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTGGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAgg < 1:219243/143‑1 (MQ=255)
aGTTTTGTGGCCGACATAGCTCACTCCAAAACGTTAAGCACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGt < 2:546559/143‑1 (MQ=255)
tgttgCAGACATAGTTCATGCGAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCcgc < 2:578997/143‑1 (MQ=255)
aGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGAGCTCCGCTGGGGCGATGCGATAGCCGACTAACTCTTGCTCCCTTCGCAGGAATTAGCCTGATCAGGTACCGCGGATcc > 2:498918/1‑143 (MQ=255)
cATGGCTCATTCCAAAAAGTTAAGGGCGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGa < 2:615920/143‑1 (MQ=255)
ttgcAAAAGGTTGAGGTGGTCGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGt < 2:17064/140‑1 (MQ=255)
gtgcAAAAAGGTACGGACGGGGGGTGGCAGACGGCGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGt < 1:148486/140‑1 (MQ=255)
aaaaGTTAGGGTCGTGGCTTGTCAGATGATGGATGAAGCAAGAGTCGATTGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAg < 1:354052/143‑1 (MQ=255)
gTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGGCTCAGCAAg > 2:582798/1‑143 (MQ=255)
gTTAAGGACGGGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAg < 2:392883/143‑1 (MQ=255)
gACGTGGCTTGTCAGACGACGGAGGAAGCAAGAGACGTTCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAgg < 2:615754/143‑1 (MQ=255)
ttGTCATGCGACGTATGAAGCAAGGCACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGa < 2:522786/143‑1 (MQ=255)
ttGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGa < 1:454481/143‑1 (MQ=255)
gTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGACGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTACGACa > 1:482716/1‑143 (MQ=255)
acgacgGCTGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGaaa < 1:252667/143‑1 (MQ=255)
aGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCa > 2:561246/1‑143 (MQ=255)
ggtggatGGCAGGTTGACTCTTGCTCCCGTCGCAGGTAGTAGCCTGATCAGGTGCCGCGGATCCCGAATAAACGGTCTCAGCCATTTAAGGCACGCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTAcgc < 2:280450/139‑1 (MQ=255)
gatAGCAGATTAACTCTTGCTCACTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAACTGGGGCCTAGAATGTGAAGAAcgcgaca > 2:496605/1‑140 (MQ=255)
aGATTACCGCTTGCTCCCTTCGCAGGTATTGGCCTGATCAGGTTCCGCCGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAgcg < 2:569932/143‑1 (MQ=255)
aTTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGTTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAACTTACGCGTTAACAGCGCa > 2:457480/1‑143 (MQ=255)
aTTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCa > 1:550099/1‑143 (MQ=255)
|
CCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCA > NC_000913/4196057‑4196327
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
