breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 125,387 C→T Q791* (CAG→TAG)  aceE → pyruvate dehydrogenase E1 component
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 696,470 C→T noncoding (35/75 nt) glnX ← tRNA‑Gln
RA 710,436 G→A H71Y (CAC→TAC)  fur ← DNA‑binding transcriptional dual regulator Fur
RA 759,250 G→T R182L (CGC→CTC)  sucA → subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
JC JC 1,293,032 IS1 (–) +8 bp intergenic (‑110/‑488) hns ← / → tdk DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
MC JC 1,299,499 Δ1,199 bp insH21 insH21
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
JC JC 1,979,486 IS5 (+) +4 bp intergenic (‑271/‑264) insA‑5 ← / → uspC IS1 protein InsA/universal stress protein C
RA 2,132,787 A→C I204S (ATC→AGC)  wcaA ← putative colanic acid biosynthesis glycosyl transferase
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
JC JC 2,391,775 IS1 (+) +9 bp coding (1259‑1267/1530 nt) nuoM ← NADH:quinone oxidoreductase subunit M
RA 2,661,969 C→G V55L (GTT→CTT)  iscR ← DNA‑binding transcriptional dual regulator IscR
RA 3,041,632 T→A V107E (GTG→GAG)  ygfZ → folate‑binding protein
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,637,646 +T coding (5/1500 nt) pitA → metal phosphate:H(+) symporter PitA
MC JC 3,815,859 Δ82 bp [rph] [rph]
MC JC 4,001,645 Δ5 bp coding (220‑224/951 nt) corA → Ni(2(+))/Co(2(+))/Mg(2(+)) transporter
RA 4,072,858 G→A A238A (GCC→GCT yihU ← 3‑sulfolactaldehyde reductase
RA 4,100,717 G→T intergenic (‑192/‑93) rhaT ← / → sodA rhamnose/lyxose:H(+) symporter/superoxide dismutase (Mn)
RA 4,158,785 C→T P99L (CCC→CTC)  oxyR → DNA‑binding transcriptional dual regulator OxyR
RA 4,184,543 C→A P1100Q (CCG→CAG)  rpoB → RNA polymerase subunit beta
RA 4,196,196 T→C intergenic (‑97/+136) thiC ← / ← rsd phosphomethylpyrimidine synthase/regulator of sigma D
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,341,824 T→G intergenic (‑196/‑87) melR ← / → melA DNA‑binding transcriptional dual regulator MelR/alpha‑galactosidase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 365913 366507 595 11 [9] [10] 11 [lacZ]–[lacI] [lacZ],[lacI]
* * ÷ NC_000913 3423754–3424234 3424536–3424238 5–783 11 [10] [10] 11 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 315572NA (NA)8 (0.240) 8/262 2.6 NA noncoding (344/1255 nt) IS3 repeat region
?NC_000913 = 315588 NA (NA)noncoding (360/1255 nt) IS3 repeat region
* ? NC_000913 566777 =NA (NA)1496 (42.040) 255/280 0.0 97.2% noncoding (1/1258 nt) IS3 repeat region
?NC_000913 = 572471 43 (1.200)coding (6/456 nt) ybcN DLP12 prophage; DNA base‑flipping protein
* ? NC_000913 1436548 =25 (0.700)191 (5.690) 125/264 0.0 86.7% coding (346/1134 nt) ompN outer membrane porin N
?NC_000913 = 1502024 35 (1.040)coding (463/594 nt) tehB tellurite methyltransferase