Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F25 I1 R1
|
24 |
37.7 |
1533230 |
90.5% |
1387573 |
141.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,196,220 |
C→T |
H366H (CAC→CAT) |
icd → |
isocitrate dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,196,220 | 0 | C | T | 96.0%
| 51.9
/ NA
| 26 | H366H (CAC→CAT) | icd | isocitrate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (1/0); major base T (16/8); minor base G (0/1); total (17/9) |
| Fisher's exact test for biased strand distribution p-value = 3.60e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATCGGTATCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTC > NC_000913/1196080‑1196301
|
accggtatcgCCCCCGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCCCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCatat < 1:374021/141‑1 (MQ=255)
cggtatcgCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATgg > 1:567682/1‑143 (MQ=255)
gtatcgCCCCTGGTGCAAACATCGATGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACGGCGCCGAAATATGCCGGTCAGGACAACGTAAATCCTGGCGCTATGATTCACTCAGCTGAGATGATCCTGCGCcacaacagc > 2:612584/1‑138 (MQ=255)
atcgCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCAACATTAAATTCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTg < 1:557630/143‑1 (MQ=255)
cgCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGACATATGCCGGTCAGGCCCACGTAAATCCTGGCCCTATTATTCTCCCCGCTGAGATGCTGCTGCGCCACATggcgtcga > 2:65420/1‑137 (MQ=255)
gCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCCCTATTATTCTCTCCGCTGAGATGATGCTACGCCATCTGGGCTGGAc > 2:167021/1‑143 (MQ=255)
tGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCgg > 1:531001/1‑143 (MQ=255)
accctCGGTGAAGGATGGCCGCTGCTTGGAGCCAGCCGCGGTACTGCGCCGAAATATGCCTGCCAGGACAAAGGACATCCTGGCTCTGTCCTTCTCCCCGCTGTGATGATCCTGCGCCATATGGGTTGGACTGAAGCGGCTGa < 2:514157/139‑1 (MQ=255)
aCATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGAc < 1:669784/143‑1 (MQ=255)
tgTGGCGAAGGCGCCCGGTTTGACGCAGCCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAAGCCTGGCTCTATTATGCTCTCCGCTGAGATGATGCTGCGCCAGATGGGTTGGACTGAAGCGGCTGAcctgat < 2:295438/142‑6 (MQ=37)
aggcgcCCGGTTCGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAg < 1:7264/141‑1 (MQ=37)
gcCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGCAGCGGCTGCCCTGATTGTTACAGGTAt > 1:85883/1‑143 (MQ=38)
ccTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGCTTGTTAAATGTATgg > 2:115500/1‑143 (MQ=37)
ccTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATgg > 1:423066/1‑143 (MQ=255)
tttGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAgg > 1:446172/1‑143 (MQ=255)
tGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAGGCGGCTGACCTGATTGTTAAAGGTCTGGAAGgcg > 1:24748/1‑143 (MQ=37)
aGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCaa > 1:43807/1‑143 (MQ=255)
aGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCaa > 1:467772/1‑143 (MQ=255)
ccacccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGAGGATGCTGCGCCATCTGGGTTGGACTCACGCGGCTGACCTGCTTGTTAAAGGTATGGAAAGCGCAATc > 2:382729/1‑143 (MQ=255)
cGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTAGCTCTATTATTCTCTCCGCTGAAATGATGCTGCGACATATGGGTTGGACTGAAGCGGCTGACCTGCTTGTGAAAGTGATGGGAGGAGCAATCAAtgcaa > 2:309651/1‑141 (MQ=21)
tACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGGCCGGATTGTTAAAGCTATGGAAGGCGCAATCAATGCCaaga > 1:172073/1‑141 (MQ=21)
aCTGCGCCGAAATAAGCCGGTCAGCACAAAGTGCCTCCCAGCGCTATTATTCTCTCCCCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGAc < 2:739927/143‑1 (MQ=14)
tGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGCTTGTTAAAGGTATGGAAGGCGCAATCCATGCCAAGACCg > 1:53008/1‑143 (MQ=18)
gcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTaa > 1:490322/1‑143 (MQ=18)
ccGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAAc > 1:257935/1‑143 (MQ=18)
ggAAGTATGCCGGTCAAGACAGAGTACATCGTGGCTCTATTATTCTCTCCGCTGAGATGAGGCTGCGCCATATGGGTTGGCCTGCAGCCGCTGCCCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAAct < 2:712338/142‑2 (MQ=14)
aacatGCCGGGCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGGTGGACTGAAGCGGCTGACCTGATTGTTAAAGGGATGGAAGGCGCAATCAATGCCAAGACCGTAACTTAt < 1:167021/140‑1 (MQ=17)
ccGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCGCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTc < 2:567682/143‑1 (MQ=14)
|
ATCGGTATCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTC > NC_000913/1196080‑1196301
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
