Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A0 F0 I1 R1	29	281.6	8141248	99.5%	8100541	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	2,480,667	Δ1 bp	intergenic (+42/+83)	zwf → / ← gndA	glucose‑6‑phosphate 1‑dehydrogenase (NADP‑dependent)/NADP+‑dependent 6‑P‑gluconate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	2,480,667	0	T	.	98.9%	850.3 / 2.3	175	intergenic (+42/+83)	zwf/gndA	glucose‑6‑phosphate 1‑dehydrogenase (NADP‑dependent)/NADP+‑dependent 6‑P‑gluconate dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (0/2); new base . (79/93); total (80/95)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 180 total aligned reads displayed.

GCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAATAGTTTACTAAGCATTTTCGGTCTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGCTCATAAGTATGAGCGC  >  BS168_reference_genome/2480521‑2480811

gcagcaAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGTTGATCGTTTGGTGAAAGCCGGCTTACACTGGTGGAACATATAATAAGAAG‑AAAAATCCGAAAATGTTTAC‑AAGCATTTTCGG‑Cttttt                                                                                                                                            <  1:3670035/150‑1 (MQ=255)

gcagcaAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGACGAAAATGTTTAC‑AAGCATTTTCGG‑Ctttt                                                                                                                                             >  1:1077107/1‑150 (MQ=255)

 cagcaAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑Cttttt                                                                                                                                            <  2:3249264/150‑1 (MQ=255)

    caaacaaaACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTAc                                                                                                                                         >  2:306603/1‑150 (MQ=255)

      aacaaaACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGc                                                                                                                                       <  2:3227045/150‑1 (MQ=255)

       ccaaaTCCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGTCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCt                                                                                                                                      <  1:2499939/149‑1 (MQ=255)

          aaaCCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGaa                                                                                                                                   >  1:360529/1‑150 (MQ=255)

           aaCCTTACCTCCTAACTCCGAATCAGGCTCAATGGGACCGGAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGATTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAAc                                                                                                                                  <  1:1101941/150‑1 (MQ=255)

             cctaacctcCAAACTACGAATCAGGCCCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaa                                                                                                                                <  2:3495812/144‑1 (MQ=255)

             ccTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaa                                                                                                                                >  1:3745070/1‑150 (MQ=255)

              cTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaaa                                                                                                                               >  2:1063851/1‑150 (MQ=255)

              cTTATCTCCTAACTACGAATCAGGCCCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaaa                                                                                                                               <  2:2452978/150‑1 (MQ=255)

               ttATCTCCTAACTACGAACCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAg                                                                                                                              <  2:3248653/150‑1 (MQ=255)

                tacctcCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCATACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAgg                                                                                                                             <  2:66683/147‑1 (MQ=255)

                  tctcCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGta                                                                                                                           >  2:1177324/1‑150 (MQ=255)

                   ctcCTACCTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGtat                                                                                                                          <  2:557039/150‑1 (MQ=255)

                     tcTAACTACGAATCAGGGACAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACTGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGCAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATaa                                                                                                                        <  1:1410755/149‑1 (MQ=255)

                     ccTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATaa                                                                                                                        <  1:884248/150‑1 (MQ=255)

                     ccTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATaa                                                                                                                        >  2:3981633/1‑150 (MQ=255)

                     ccTAACTACGAATCAGGCCCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATaa                                                                                                                        <  1:1260691/150‑1 (MQ=255)

                      cTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CCTTTTTACGCTGAACAAAGGTATAAc                                                                                                                       >  1:1707365/1‑150 (MQ=255)

                       tAACTACGAATCAGGCACAATGGGACCGAAAGACCCTCATGTCCTTTTGGTGAAAGGCGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAGATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACa                                                                                                                      <  2:3887899/150‑1 (MQ=255)

                         aCTACGAATCAGGCTCAATGGGACCGAAAGAATCTGTTGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAAt                                                                                                                    <  1:2709108/150‑1 (MQ=255)

                         aCTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAAt                                                                                                                    <  2:157213/150‑1 (MQ=255)

                         aCTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTGTACGCTGAACAAAGGTATAACAAt                                                                                                                    >  2:3281085/1‑150 (MQ=255)

                          caaCGAATCAGGCCCAATGGGACCGAATGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAAtc                                                                                                                   <  1:2984518/148‑1 (MQ=255)

                          cTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAAtc                                                                                                                   >  2:273463/1‑150 (MQ=255)

                          cTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAAtc                                                                                                                   <  1:1772876/150‑1 (MQ=255)

                             cGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTcc                                                                                                                <  1:3343215/150‑1 (MQ=255)

                             cGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTcc                                                                                                                <  1:2385488/150‑1 (MQ=255)

                               aaccAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCtt                                                                                                              <  2:745073/147‑1 (MQ=255)

                                      cTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACa                                                                                                       >  2:1227202/1‑150 (MQ=255)

                                       tCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACaa                                                                                                      >  2:2703357/1‑150 (MQ=255)

                                        cAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGGCGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAGAATGTTTTC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACaaa                                                                                                     <  1:523759/150‑1 (MQ=255)

                                        cAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACaca                                                                                                     >  1:2800049/1‑148 (MQ=255)

                                         aaTGGGACCGAAGGAATCTGATGACCTTTTGGTGAAAGCCGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACaaaa                                                                                                    <  2:1619425/150‑1 (MQ=255)

                                         aaTGGGACCGAAAGAATCTGATGATCTTTTGGTGTAAGACGGCTTACACTGGTGGAACATATAATAAGAAGTGAAAAGCCGAAAATGTTTAC‑AAGCAGTTTCGG‑CGTGTTTTCGCTGAACAAAGGTATAACAATCTCCTTAATAACcaaa                                                                                                    >  2:542488/1‑150 (MQ=255)

                                         aaTGGGACCGAAAGAATCTGATGACCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACaaaa                                                                                                    <  2:1169117/150‑1 (MQ=255)

                                           tGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACaaaa                                                                                                    <  1:3693699/148‑1 (MQ=255)

                                           tGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAAcc                                                                                                  <  1:1617349/150‑1 (MQ=255)

                                            gggACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAAccc                                                                                                 >  2:1013323/1‑150 (MQ=255)

                                              gACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAAccccc                                                                                               <  1:976778/150‑1 (MQ=255)

                                               aCCGAAAGATTCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCa                                                                                              <  1:3173340/150‑1 (MQ=255)

                                                  gAAAGAATCTGATGATCTTTTGGTGAAGGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAg                                                                                           <  1:1205372/150‑1 (MQ=255)

                                                    aaGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCAATTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCt                                                                                         >  2:1370828/1‑150 (MQ=255)

                                                     aGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCtt                                                                                        <  2:1060215/150‑1 (MQ=255)

                                                           tgatgaTCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGc                                                                                  >  1:876405/1‑150 (MQ=255)

                                                            gatgatCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCt                                                                                 >  1:3494314/1‑150 (MQ=255)

                                                             atgatCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCtt                                                                                >  2:2847846/1‑150 (MQ=255)

                                                             atgatCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCtt                                                                                >  2:16590/1‑150 (MQ=255)

                                                                 tCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCggg                                                                            <  2:501227/150‑1 (MQ=255)

                                                                  cTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCgggg                                                                           <  2:2910757/150‑1 (MQ=255)

                                                                    tttGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGt                                                                         >  2:3188343/1‑150 (MQ=255)

                                                                      tGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGttt                                                                       <  2:1979926/150‑1 (MQ=255)

                                                                       ggTGAAAGACGGCTTACACTGGTGGAACAAATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATAGT‑GGGCTTTTTTACTCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAACAGCTTCGGGGGTTTc                                                                      >  2:2219227/1‑150 (MQ=255)

                                                                        gTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGGTTCt                                                                     >  2:2472685/1‑150 (MQ=255)

                                                                        cggAAACACGGCTTACTCTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTGTAC‑AAGCATTTTC‑GTGTTTTTTACGCTGAACAAAGGTATAACAAACTCCTTAATAACAAAACATCCAAAGCTTAAGAGCTTCGGGGGtttgt                                                                     <  2:626759/148‑3 (MQ=255)

                                                                           aaaGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTAtt                                                                  >  2:2757852/1‑150 (MQ=255)

                                                                                cGGCTTACACTGGTGGAACATATAATAAGAAGAAACAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTa                                                             <  1:1393181/150‑1 (MQ=255)

                                                                                 ggCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTAc                                                            <  1:2547446/150‑1 (MQ=255)

                                                                                   cTTACACTGGTGGAACATATAATAAGAAGAAAAAACCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACtt                                                          <  1:1290452/150‑1 (MQ=255)

                                                                                    ttACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTAAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTc                                                         <  1:417572/150‑1 (MQ=255)

                                                                                       cacTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATc                                                      >  2:2534757/1‑150 (MQ=255)

                                                                                       cacTGGTGGAACATAAAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATc                                                      <  1:914121/150‑1 (MQ=255)

                                                                                            gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTc                                                 <  1:1515122/150‑1 (MQ=255)

                                                                                            gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTc                                                 <  2:960417/150‑1 (MQ=255)

                                                                                            gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGGTTCTATTGATTACTTCATCCATTc                                                 >  2:3609157/1‑150 (MQ=255)

                                                                                              ggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGGTTACTTCATCCATTCAg                                               >  1:2175800/1‑150 (MQ=255)

                                                                                                 aCAAATAATAAGAAGAAAAAGGCCGAAATTGTTTAC‑AAGCATTTTCTG‑CTTTTTTACGCAGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTAt                                            <  1:3854157/150‑1 (MQ=255)

                                                                                                  cATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCAACCATTCAGTATg                                           >  2:2100302/1‑150 (MQ=255)

                                                                                                   ataaAATAGGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTAACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATgg                                          <  1:3284206/150‑1 (MQ=255)

                                                                                                    tataATAAGAAGAAATAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGa                                         <  1:77041/150‑1 (MQ=255)

                                                                                                    tataATAAGAAGAAAAACGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAAAAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTAATGATTACTTCATCCATTCAGTATGGa                                         <  1:3145037/150‑1 (MQ=255)

                                                                                                     ataataAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGCGTTTCTATTGATTACTTCATCCATTCAGTATGGaa                                        >  2:1095516/1‑150 (MQ=255)

                                                                                                      taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAg                                       <  1:3642398/150‑1 (MQ=255)

                                                                                                         taaGAAGAAAAAAGCCGAAATTGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATg                                    <  2:1861348/150‑1 (MQ=255)

                                                                                                          aagaagAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGc                                   <  2:3404232/150‑1 (MQ=255)

                                                                                                           agaagaAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTATTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGcc                                  <  1:1825517/150‑1 (MQ=255)

                                                                                                            gaagaaAAAAGCCGCAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCct                                 <  2:2062852/150‑1 (MQ=255)

                                                                                                            gaagaaAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCct                                 <  2:2344508/150‑1 (MQ=255)

                                                                                                             aagaaAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCctt                                <  1:2623828/150‑1 (MQ=255)

                                                                                                                aaaaaaGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCcttctt                             >  2:3178244/1‑150 (MQ=255)

                                                                                                                aaaaaaGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCcttctt                             >  2:152642/1‑150 (MQ=255)

                                                                                                                  tacaGCCGAAAATGATTAC‑AAGCATTTTCGG‑CCAATTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTg                           >  2:2609983/4‑150 (MQ=255)

                                                                                                                      gCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAg                       >  2:1813211/1‑150 (MQ=255)

                                                                                                                       ccGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTATCAGt                      >  1:2060476/1‑150 (MQ=255)

                                                                                                                          aaaatgTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACg                   <  1:3004148/145‑1 (MQ=255)

                                                                                                                           aaatgTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGc                  >  2:3817551/5‑150 (MQ=255)

                                                                                                                                gattacaaGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATACCAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGCTCAt              <  2:2883850/144‑1 (MQ=255)

                                                                                                                                    acaaGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTAGAGGGCTTCGGGGGTGTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGCTCATAAGt          >  2:3761326/3‑150 (MQ=255)

                                                                                                                                     caaGCGTTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACACTCCCCTTAAAAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTAATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGCTCATAAGTa         <  2:2472210/149‑1 (MQ=255)

                                                                                                                                      aaGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGCTCATAAGTAt        >  1:3962207/1‑150 (MQ=255)

                                                                                                                                      aaGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGCTCATAAGTAt        <  1:3535795/150‑1 (MQ=255)

                                                                                                                                      aaGCATTTCCGG‑CTTTTTGACGCTGAACAAAGGTATAACAATCTACTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATGCAGTATGGAAGATGCCTTCTTTGTCAGTACGCTCATAAGTAt        <  2:4028614/150‑1 (MQ=255)

                                                                                                                                       aGCATTTCCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGCTCATAAGTATg       <  2:360529/150‑1 (MQ=255)

                                                                                                                                         cATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGCTTACTTCATCCATTCAGTATGGAAGATGCCTCCTTTGTCTGTACGCTCATAAGAATggg     >  1:3067126/1‑148 (MQ=255)

                                                                                                                                          aTTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGGAGATGCCTTCTTTGTCAGTACGCTCATAAGTATGAgc    >  2:3052528/1‑150 (MQ=255)

                                                                                                                                          aTTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGCTCATAAGTATGAgc    >  2:175575/1‑150 (MQ=255)

                                                                                                                                            tttCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGCTCATAAGTATGAgcgc  >  2:3731356/1‑150 (MQ=255)

                                                                                                                                            tttCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGCTCATAAGTATGAgcgc  <  1:3817551/150‑1 (MQ=255)

GCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAATAGTTTACTAAGCATTTTCGGTCTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGCTCATAAGTATGAGCGC  >  BS168_reference_genome/2480521‑2480811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A