Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 29 281.6 8141248 99.5% 8100541 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA BS168_reference_genome 2,581,732 (T)6→7 intergenic (‑115/+39) pstS ← / ← pbpA phosphate ABC transporter (phosphate binding lipoprotein)/transpeptidase (penicillin‑binding protein 2A)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome2,581,7261.T91.5% 494.7 / 37.5 142intergenic (‑109/+45)pstS/pbpAphosphate ABC transporter (phosphate binding lipoprotein)/transpeptidase (penicillin‑binding protein 2A)
Reads supporting (aligned to +/- strand):  ref base . (5/7);  new base T (47/83);  total (52/90)
Fisher's exact test for biased strand distribution p-value = 7.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

Only 100 of 153 total aligned reads displayed.

GCAGCCATCAGAAGCATAAGCACCAATTTGTTTTTTTTCATTTCCTGAATTCCCCCTGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑G‑T‑T‑TT‑TT‑GTATAAAAAAAACGCCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACCTTCTTTGTCCTGCTTCGATTTCAGCTCAAA  >  BS168_reference_genome/2581577‑2581869
                                                                                                                                                       |                                                                                                                                                   
gcagcCATCAGAAGCATAAGCACCAATTTGTTTTTTTTCATTTCCTGAATTCCCCCTGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑g                                                                                                                                                      <  1:405146/150‑1 (MQ=255)
gcagcCATCAGAAGCATAAGCACCAATTTGTTTTTTTTCATTTCCTGAATTCCCCCTGCGTATAATGTGTAATTTGTCCTACACGAATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑g                                                                                                                                                      <  1:3917092/150‑1 (MQ=255)
 cagcCATCAGAAGCATAAGCACCAATTTGTTTTTTTTCATTTCCTGAATTCCCCCTGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑G‑t                                                                                                                                                    <  1:1090375/150‑1 (MQ=255)
  agcCATCAGAAGCATAAGCACCAATTTGTTTTTTTTCATTTCCTGAATTCCCCCTGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑G‑t‑t                                                                                                                                                  >  1:173994/1‑150 (MQ=255)
       tCAGAAGCATAAGCACCAATTTGTTTTTTTTCATTTCCTGAATTCCCCCTGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑G‑t‑t‑tt‑ttt                                                                                                                                           <  1:3002529/150‑2 (MQ=255)
        cAGAAGCATAAGCACCAATTTGTTTTTTTTCATTTCCTGAATTCCCCCTGCGTATAATGTGTAATTTGTCCTACAGGTATAGAGTAAAGGTTCCATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑G‑t‑t‑tt‑tttg                                                                                                                                          <  1:827264/150‑3 (MQ=255)
          gAAGCATAAGCACCAATTTGTTTTTTTTCATTTCCTGAATTCCCCCTGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑G‑t‑t‑tt‑tttgta                                                                                                                                        <  2:633116/150‑5 (MQ=255)
           aaGCATAAGCACCAATTTGTTTTTTTTCATTTCCTGAATTCCCCCTGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑G‑t‑t‑tt‑tttgtat                                                                                                                                       <  2:710776/150‑6 (MQ=255)
             gCATAAGCACCAATTTGTTTTTTTTCATTTCCTGAATTCCCCCTGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATaa                                                                                                                                     <  2:169635/150‑1 (MQ=255)
               aTAAGCCCCAATTTGTTTTTTTTCATTTCCTGAATTCCACCTGCCTATAATGTGTAATTTGTCCTGCACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATaaac                                                                                                                                   <  1:647206/150‑2 (MQ=255)
               aTAAGCACCAATTTGTTTTTTTTCATTTCCTGAATTCCCCCTGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATaaaa                                                                                                                                   >  1:1746702/1‑150 (MQ=255)
                 aaGCACCAATTTGTTTTTTTTCATTTCCTGAATTCCCCCTGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATaaaaaa                                                                                                                                 <  1:2189962/150‑1 (MQ=255)
                  aGCACCAATTTGTTTTTTTTCATTTCCTGAATTCCCCCTGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAACGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATaaaaaaa                                                                                                                                <  1:2626294/150‑1 (MQ=255)
                   gCACCAATTTGTTTTTTTTCATTTCCTGAATTCCCTCTGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATaaaaaaga                                                                                                                               <  1:1260810/150‑3 (MQ=255)
                      ccAATTTGTTTTTTTTCATTTCCTGAATTCCCCCTGCGTATATTGTGGAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGc                                                                                                                            >  1:3596543/1‑150 (MQ=255)
                      ccAATTTGTTTTTTTTCATTTCCTGAATTCCCCCTGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGc                                                                                                                            >  1:1785133/1‑150 (MQ=255)
                        attttGTTTTTTTTCATTTCCTGAATTCCCCCTGCGTTTAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGccc                                                                                                                          <  2:1867896/148‑1 (MQ=255)
                        aaTTTGTTTTTTTTCATTTCCTGAATTCCCCCTGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGccc                                                                                                                          >  1:202127/1‑150 (MQ=255)
                         aTTTGTTTTTTTTCATTTCCTGAATTCCCCCTGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGCCca                                                                                                                         >  1:1031896/1‑150 (MQ=255)
                         aTTTGTTTTTTTTCATTTCCTGAATTCCCCCTGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGCCca                                                                                                                         <  1:2034650/150‑1 (MQ=255)
                         aTTTGTTTTTTTTCATTTCCTGAATTCCCCCTGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGATTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAGAAAAACGCCca                                                                                                                         >  1:348995/1‑150 (MQ=255)
                          tttGTTTTTTTTCATTTCCTGAGTTCCCCCTGCCTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAACGGGTTGAACCGAATGTTAAGGTTTTGTAAATCAGGGCGG‑T‑T‑TT‑TT‑GGATAAAAAAAACGCCcac                                                                                                                        >  2:2076404/1‑150 (MQ=255)
                              tttttttcCTTTTCCTGAATTCCCCCTGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTCCAATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGCCCAcatca                                                                                                                    <  2:567555/150‑1 (MQ=255)
                              tttttgttCATTTCCTGATTTGCCCCTGCGTATAATGTGTACTTTGCCCTACGCGTATAGACTAAAGTTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAAGAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAATACGCCCAcatca                                                                                                                    <  2:621643/150‑1 (MQ=255)
                               ttttttcCCTTCCCTGAATTCCCCCTGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAACACGCCCAcatcat                                                                                                                   <  2:2883453/150‑1 (MQ=255)
                                   tttCATTTCCTGAATTCCCCCTGCGTATAATGTGTAATTTGTCCTCCACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAACCGCCCACATCATGTGa                                                                                                               <  1:2510360/150‑1 (MQ=255)
                                   ttccATTTCCTGAATTCCCCCTGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAGACGCCCACATCATGTGa                                                                                                               <  1:140857/147‑1 (MQ=255)
                                    ttCATTTCCTGATTTCCCCCTGCGTATAATGTGTAATTTGTCCAACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAACACGCCCACATCATGTGAg                                                                                                              <  2:4034388/150‑1 (MQ=255)
                                     tCATTTCCTGAATTCCCCCTGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGCCCACATCATGTGAGc                                                                                                             >  2:2821271/1‑150 (MQ=255)
                                      cATTTCCTGAATTCCCCCTGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGCCCACATCATGTGAGCg                                                                                                            <  2:2762019/150‑1 (MQ=255)
                                      cATTTCCTGAATTCCCCCTGCGTATAATGTGCAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑G‑T‑T‑TT‑TTCGTTTATTGTAAACGCCCACATCATGTGGGCg                                                                                                            >  1:2937514/1‑150 (MQ=255)
                                       ttttCCTGAATTCCCCCTGCGTATAATGTGTAATTGGTCCTACACGTATAGAGTAGAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAACACGCCCACATCATGTGAGCGt                                                                                                           <  1:3350142/149‑1 (MQ=255)
                                        ttttCTGAATTCCCATTGTTTATAATGTGTATTTTGTCCTACAGGTATAGAGTAATGGTTCTATGTAAAAAGGGTTTGAACCAAATTTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAACACTCCCACATCATGTGAGCGtt                                                                                                          <  1:1333677/150‑1 (MQ=255)
                                             tGAATTCCCCCTTGGTATAATGTGTAATTTGTCCTCCACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGATTGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGCCCACATCATGTGAGCGttttttt                                                                                                     <  1:3936453/150‑1 (MQ=255)
                                              gAATTCCCCCTGCGTATAATGTGTAATTTGTCCAACACGTATAGAGTAAAGGTTCAATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAACACGCCCACATCATGTGAGCGtttttttt                                                                                                    <  1:3621559/150‑1 (MQ=255)
                                                   cccccTGCGTATAGTGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGCCCACATCATGTGAGCGTTTTTTTTTAGtt                                                                                               <  1:967808/150‑1 (MQ=255)
                                                   cccccTGCGTATAATGTGTAATTTGCCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAACACGCCCACATCATGTGAGCGTTTTTTTTTAGtt                                                                                               <  1:2274866/150‑1 (MQ=255)
                                                      ccTGCGTATAAGGTGTATTTTGCCCTCCACGTATAGAGTAAGGGTCCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGCCCACATCATGTGAGCGTTTTTTTTTAGTTATc                                                                                            <  2:2711582/150‑1 (MQ=255)
                                                        tGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGCCCACATCATGTGAGCGTTTTTTTTTAGTTGTCag                                                                                          >  2:3129405/1‑150 (MQ=255)
                                                           gTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAACACGCCCACATCATGTGAGCGTTTTTTTTTAGTTATCagaag                                                                                       <  2:3872047/150‑1 (MQ=255)
                                                           gTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACACCCACATCATGTGAGCGTGTTTTTTTAGTTATCcgaag                                                                                       >  1:3040196/1‑150 (MQ=255)
                                                           gTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGGTTGAACCGAATGTCAAGGTTTTGTAAATCAAGGC‑G‑TCT‑AT‑TT‑GTAAACAAAAAACGCCCCAATCAGGTGAGCATTTATTTTTAGTTATCagaaa                                                                                       >  2:1156746/1‑149 (MQ=255)
                                                           gTATAATGTGTAATTAGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAACCGCCCACATCATGTGAGCGTTTTTTTTTAGTTATCagaag                                                                                       <  1:2814755/150‑1 (MQ=255)
                                                                  gtgtAATTTGTCCTACTCGTTTAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAACTCCGCCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTtgt                                                                                <  1:1039859/150‑1 (MQ=255)
                                                                   tgtAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAACACGCCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTtgtg                                                                               <  2:3201439/150‑1 (MQ=255)
                                                                    gtAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGCCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTgtgt                                                                              <  1:3756379/150‑1 (MQ=255)
                                                                      aaTTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGCCCACATCATGGGAGCGTTTTTCTTTAGCTTTCAGAAGACGGtgtgagt                                                                            >  2:3716361/1‑147 (MQ=255)
                                                                               tcccggTATAGGGTAGTGGTTCTAGTTGAGAAGGGTGTGACCGGGATGTTAAGGTTTTGTAAATGAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAGACCCCCACATCATGTGAGGGTTGTTTCTTGGTAACCAGAAGTCGTTGTGTTTTCTGCAttt                                                                   <  2:3044523/145‑1 (MQ=255)
                                                                                  acGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGCCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttct                                                                <  2:2685867/150‑1 (MQ=255)
                                                                                   cGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGCCCACATCATGTGAGCGGTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttctt                                                               >  2:2038496/1‑150 (MQ=255)
                                                                                   cGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAACCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGCCCACGTCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttctt                                                               <  2:1051542/150‑1 (MQ=255)
                                                                                     tttaGAGTAAAGGTTCTATGTAAAAGGGGTTTGAACCGAATGTTAGGGTTTTGTAATTCAGTGG‑GTT‑T‑TT‑TT‑GTATAAAAAACACACCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCa                                                             <  1:3824439/148‑1 (MQ=255)
                                                                                       taGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑G‑T‑TGTT‑TT‑GTATAAAAAAAACGCCCACATCATGTGAGCGGTTTTTTTTAGTTTTCAGGAGAGGTGGGGTTTTCTGCCGTTTCTTCAAt                                                           >  2:3598578/1‑150 (MQ=255)
                                                                                        agagTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGCCCACATCATGTGAGCGTCTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAAtt                                                          >  1:1193499/1‑150 (MQ=255)
                                                                                         gagTAAAGGTCCTATGTAAAAAGGGTTTGACCCGAATGTTAAGGTTTTGTAAATCAGTGG‑GTT‑T‑TT‑TT‑GTATAAAAAACTCGCCCCCATCATGTGAGCGTTTTTTTTTAGATATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAAttt                                                         <  2:879266/150‑1 (MQ=255)
                                                                                           gTAGAGGTTCTATGTAAGAAGGGTTTGAACCGAGTGTTAAGGTTTTGTAAATTAGTGG‑GTT‑T‑TT‑TT‑GTATAAAAAAAGCGCCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTg                                                       <  2:1972580/150‑1 (MQ=255)
                                                                                           gTAAAGGTCCATTGAAAAAAGGGTTTGAACGGATTGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACCCCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTg                                                       <  1:611051/150‑1 (MQ=255)
                                                                                            tgagggTTCTATGTAAAAAGGGTTTGACCGGAATGTTAGGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAACCGCCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGt                                                      <  2:1805548/146‑1 (MQ=255)
                                                                                             aaaGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGCCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTc                                                     >  2:1986331/1‑150 (MQ=255)
                                                                                              aaGGTTCTTTGTAAAGAGGGTTTGAACGGAATGTTAAGGTTTTTTAACTCGTTGT‑GTT‑T‑TT‑TT‑GTATAAAAAATACCCCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCt                                                    <  1:2435664/150‑1 (MQ=255)
                                                                                                  ttCTATGTAAAAAGGGTTTGACCCGAATGTTAAGGTTTTGTAAACCAGTGC‑GTT‑T‑TT‑TT‑GAATAAAACAAACGCCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTAt                                                <  2:315227/150‑1 (MQ=255)
                                                                                                  ttCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGCCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCTTTTTCTTCAATTTTGTCTTTAt                                                >  1:100402/1‑150 (MQ=255)
                                                                                                   tCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCGGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAACACGCCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTAtt                                               <  2:1180270/150‑1 (MQ=255)
                                                                                                   tCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGCCCACATCATGTGAGCGTGTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCATTTTTGTCTTTAtt                                               >  1:3400785/1‑150 (MQ=255)
                                                                                                   tCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGCCCACATCATGTGAGCGACTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTAtt                                               >  1:1501938/1‑150 (MQ=255)
                                                                                                     tATGTAAAAAGGGTTTGAACTGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAACCGCCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCt                                             <  1:2033152/150‑1 (MQ=255)
                                                                                                      aTGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGCCCACATCATGTGAGCGTGTTTTTTTAGTTATCAGAAGACGTTGGGGTTTCTGGATTTTCTTCAATTTTGGCTTTTTTCtt                                            >  2:2590224/1‑150 (MQ=255)
                                                                                                         tAAAAAGTGTTTGAACCGAATGCTAAGGTCTTGTAAATCAGTGC‑G‑T‑TCTT‑TT‑GAATAAAAAAAACGCCCACATCATGTGAGCGTATTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTg                                         >  1:1812197/1‑150 (MQ=255)
                                                                                                         tAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGCCCACATCATGTGAGCGTTTTTTTTTAGTTATCGGAAGACGTTGTGGTTTCTGCATTTTCTTCAATTTTTGCTTTATTCTTTTg                                         >  2:1600297/1‑150 (MQ=255)
                                                                                                          aaaaaGGGTTTGACCCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAGACGCCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGc                                        <  2:40784/150‑1 (MQ=255)
                                                                                                          aaaaaGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTGAAAAAAAACCGCCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGc                                        <  1:2901629/150‑1 (MQ=255)
                                                                                                           aaaaGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGCCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCt                                       >  2:611930/1‑150 (MQ=255)
                                                                                                            aaaGGGTTTGAACCGATTGTTAATGTTTTGTAATTCACTGCTT‑T‑T‑TT‑TT‑GTATAAAAAATACGCCCACATCATGTGAGCGTTTTTTTTCAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCCTTTGCTg                                      <  2:2724784/150‑1 (MQ=255)
                                                                                                            aaaGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCGGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAATACGCCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTg                                      <  2:3798864/150‑1 (MQ=255)
                                                                                                             aaGGGTTTGAACCGATTGTTAGGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAATACGCCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGa                                     <  2:3068510/150‑1 (MQ=255)
                                                                                                               gggTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGCCCACATCATGTGAGCGTGTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGt                                   >  1:2903219/1‑150 (MQ=255)
                                                                                                                 gTTTGAACCGAATGTTGAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAATACGCCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTAc                                 <  1:2716723/150‑1 (MQ=255)
                                                                                                                    tGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGCCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTGTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGGGTACctt                              >  2:3330192/1‑150 (MQ=255)
                                                                                                                    tGAACCGAATGTAAAGGTTTTGTAAATCAGTGC‑G‑T‑T‑TTCTT‑GTATAAAAATCACTCCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACctt                              <  1:4024912/150‑1 (MQ=255)
                                                                                                                      acccGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAATACGCCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACcttct                            <  1:3066636/148‑1 (MQ=255)
                                                                                                                        ccGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAATAACCCCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACCTTCttt                          <  1:2280845/150‑1 (MQ=255)
                                                                                                                        ccGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGCCCACATCATGTGAGCGATTTTTTTTAGTTATCAGAGGACGGGGTGGTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGGGGACCTTCttt                          >  2:69264/1‑150 (MQ=255)
                                                                                                                        ccGAAGGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAATACGCCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACCTTCttt                          <  1:3101264/150‑1 (MQ=255)
                                                                                                                         cGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAATCCCCCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACCTTCTTTg                         <  2:1106822/150‑1 (MQ=255)
                                                                                                                         cGAATGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGCCCACATCCTGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGACTTTATTCTTTTGGTGAGTACCTTCTTTg                         >  2:3748048/1‑150 (MQ=255)
                                                                                                                           aaggTTAGGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGCCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACCTTCTTTGTc                       <  2:3200508/147‑1 (MQ=255)
                                                                                                                           aaTGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGCCCACATCATGTGAGCGTATTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACCTTCTTTGTc                       >  1:2879842/1‑150 (MQ=255)
                                                                                                                            aTGTTAGGGGTTGGTATAACAGTGCGG‑G‑T‑TT‑TG‑GTATAGAAAAAACACCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTGTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACCTTCTTTGTcc                      <  2:348995/150‑1 (MQ=255)
                                                                                                                            aTGTTAAGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGCCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTTCTGAGTACCTTCTTTGTcc                      >  1:1417325/1‑150 (MQ=255)
                                                                                                                             tGTTAAGGTTTAGTAAATCAGTGCTT‑T‑T‑TT‑TT‑GAATAAAAAGCACACCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACCTTCTTTGTCCt                     <  1:1600297/150‑1 (MQ=255)
                                                                                                                                 aaGGTTTTGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGCCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACCTTCTTTGTCCTGCtt                 <  1:2185633/150‑1 (MQ=255)
                                                                                                                                  gggTTTTGAAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAATATACTCCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACCTTCTTTGTCCTGCTTc                <  1:1344326/149‑1 (MQ=255)
                                                                                                                                      tttGTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGCCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACCTTCTTTGTCCTGCTTCGAtt            >  1:925542/1‑150 (MQ=255)
                                                                                                                                         gTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGCCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACCTTCTTTGTCCTGCTTCGGTTTCa         >  2:2460763/1‑150 (MQ=255)
                                                                                                                                         gTAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGCCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACCTTCTTTGTCCTGCTTCGATTTCa         >  2:3844917/1‑150 (MQ=255)
                                                                                                                                          tAAATCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGCCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACCTTCTTTGTCCTGCTTCGATTTCAg        <  2:2128515/150‑1 (MQ=255)
                                                                                                                                            aaTCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGCCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACCTTCTTTGTCCTGCTTCGATTTCAGCt      <  2:1746702/150‑1 (MQ=255)
                                                                                                                                             aTCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGCCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACCTTCTTTGTCCTGCTTCGATTTCAGCTc     >  2:1844767/1‑150 (MQ=255)
                                                                                                                                              tCAGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGCACACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACCTTCTTTGTCCTGCTTCGATTTCAGCTCa    <  1:3403762/150‑1 (MQ=255)
                                                                                                                                                aGTGC‑GTT‑T‑TT‑TT‑GTATAAAAAAAACGCCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACCTTCTTTGTCCTGCTTCGATTTCAGCTCaaa  >  2:3802376/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                   
GCAGCCATCAGAAGCATAAGCACCAATTTGTTTTTTTTCATTTCCTGAATTCCCCCTGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAGTGC‑G‑T‑T‑TT‑TT‑GTATAAAAAAAACGCCCACATCATGTGAGCGTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACCTTCTTTGTCCTGCTTCGATTTCAGCTCAAA  >  BS168_reference_genome/2581577‑2581869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A