Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A21 F66 I1 R1	35	99.0	4347662	95.6%	4156364	127.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	2,581,732	(T)_6→7	intergenic (‑115/+39)	pstS ← / ← pbpA	phosphate ABC transporter (phosphate binding lipoprotein)/transpeptidase (penicillin‑binding protein 2A)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	2,581,726	1	.	T	91.8%	324.4 / 20.5	97	intergenic (‑109/+45)	pstS/pbpA	phosphate ABC transporter (phosphate binding lipoprotein)/transpeptidase (penicillin‑binding protein 2A)
Reads supporting (aligned to +/- strand): ref base . (2/6); new base T (74/15); total (76/21)
Fisher's exact test for biased strand distribution p-value = 1.13e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

AAGCATAAGCACCAATTTGTTTTTTTTCATTTCCTGAATTCCCCCTGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑G‑T‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACCTTCTTTGTCCTGCTTCG  >  BS168_reference_genome/2581588‑2581856

aaGCATAAGCACCAATTTGTTTTTTTTCATTTCCTGAATTCCCCCTGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑g                                                                                                                                          >  3:419018/1‑139 (MQ=255)

     tAAGCACCAATTTGTTTTTTTTCATTTCCTGAATTCCCCCTGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGAAAATCAG‑TGC‑G‑t‑tttt                                                                                                                                   >  1:282123/1‑139 (MQ=255)

              aTTTGTTTTTTTTCATTTCCTGAATTCCCCCTGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑aa                                                                                                                         >  6:401800/1‑138 (MQ=255)

                                              gCGTATAATGTGTAAATTCTCCTACACGTATAGAGTAAAGGTTCTATGTATAAAGGGTATGAACAGAATGTTAAGGTTTTGTAAATCAG‑TGC‑G‑T‑TTTT‑TAGTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑aattttta                                                                                       >  4:194859/1‑138 (MQ=255)

                                               cGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑ttttttttt                                                                                      >  1:121055/1‑139 (MQ=255)

                                                 tataATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAg                                                                                    >  5:57183/1‑139 (MQ=255)

                                                         gtAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTATTTATCag                                                                             >  2:510830/1‑138 (MQ=255)

                                                         gtAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCag                                                                             >  5:210677/1‑138 (MQ=255)

                                                         gtAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCag                                                                             >  7:327249/1‑138 (MQ=255)

                                                         gtAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCag                                                                             >  2:72094/1‑138 (MQ=255)

                                                         gtAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCag                                                                             >  6:130764/1‑138 (MQ=255)

                                                         gtAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TGTTTTTTTATTTATCag                                                                             >  6:322662/1‑138 (MQ=255)

                                                         gtAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TGTTTTTTTAGTTTTCag                                                                             >  1:429627/1‑138 (MQ=255)

                                                         gtAATTTGTCCTACACGTATAGAGTAAAGGGTCTATGTAAAAAGGGTTAGAACCGAATGTTAAGGTTTTGGAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TGTTTTATTAGTTATCag                                                                             >  5:210569/1‑138 (MQ=255)

                                                          tAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCag                                                                             >  7:121328/1‑137 (MQ=255)

                                                             tttGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGAc                                                                        >  5:127640/1‑139 (MQ=255)

                                                                gTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAATTCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTAATCAGAAGACGtt                                                                     >  7:323764/1‑139 (MQ=255)

                                                                gTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACg                                                                       >  7:316007/1‑137 (MQ=255)

                                                                gTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGtt                                                                     >  5:213824/1‑139 (MQ=255)

                                                                 tCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTtg                                                                    >  1:4095/1‑139 (MQ=255)

                                                                  ccTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTtgt                                                                   >  3:539684/1‑139 (MQ=255)

                                                                           ataGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTcgg                                                           >  3:397943/1‑136 (MQ=255)

                                                                           ataGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTg                                                           >  2:32437/1‑138 (MQ=255)

                                                                           ataGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTg                                                           >  7:294422/1‑138 (MQ=255)

                                                                              gagTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TGTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCAtt                                                       >  6:135141/1‑139 (MQ=255)

                                                                                gTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACTCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCAattt                                                     >  4:307293/1‑139 (MQ=255)

                                                                                gTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCAtttt                                                     >  2:335488/1‑139 (MQ=255)

                                                                                 ttaaGGTTCTATGTAAAACGGGTTTGAACAGAATGTTAAGGTTTTGTAAATCAG‑TGC‑G‑T‑TTTTAT‑GTAT‑AAAAAAAACGCCCACATCATGTGAGAG‑TTTTTTTTTACGTATCTGAACAGGTTGTGTTTTGTGCATTttc                                                    >  2:138076/3‑139 (MQ=255)

                                                                                 tAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttc                                                    >  4:438809/1‑139 (MQ=255)

                                                                                 tAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttc                                                    >  5:38502/1‑139 (MQ=255)

                                                                                 tAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttc                                                    >  3:384829/1‑139 (MQ=255)

                                                                                 tAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCAGCATTttc                                                    >  2:239530/1‑139 (MQ=255)

                                                                                   aaGGTTTTATTTAAAAAGGGTTTGAACCTAATGTTAAGGTTTTGTAAATCTT‑TGC‑GTT‑TTTT‑T‑TTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttctt                                                  <  8:294422/139‑1 (MQ=255)

                                                                                   aaGGTTCTATGTAAAAAGGGTTTTAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCATAAGACGTTGTGTTTTCTGCATTttctt                                                  >  1:303975/1‑139 (MQ=255)

                                                                                   aaGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTATAAAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttct                                                   >  3:85070/1‑139 (MQ=255)

                                                                                   aaGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTTTCAGAAGACGTTGTGTTTTCTGCAttttgt                                                   >  5:158454/1‑136 (MQ=255)

                                                                                   aaGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAATAAGTTGTTTTTTATGCAttttat                                                   >  8:332003/1‑136 (MQ=255)

                                                                                   aaGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttctt                                                  >  7:22084/1‑139 (MQ=255)

                                                                                   aaGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttctt                                                  >  6:219958/1‑139 (MQ=255)

                                                                                   aaGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttctt                                                  >  6:193076/1‑139 (MQ=255)

                                                                                   aaGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttctt                                                  >  1:504793/1‑139 (MQ=255)

                                                                                   aaGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttctt                                                  >  1:420475/1‑139 (MQ=255)

                                                                                   aaGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttct                                                   >  7:315867/1‑138 (MQ=255)

                                                                                   aaGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttct                                                   >  5:87864/1‑138 (MQ=255)

                                                                                   aaGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttct                                                   >  3:373665/1‑138 (MQ=255)

                                                                                   aaGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATAATAAGACGTTGTGGTTTATGGAatttgt                                                   >  1:538761/1‑136 (MQ=255)

                                                                                   aaGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTAAGTTATAAGAAGACGTTGTGTTTTCTGCATTtactt                                                  >  7:290957/1‑139 (MQ=255)

                                                                                    aGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTTGCAGAAGACGTTGTGTGTTCTGCATTttctt                                                  >  4:285129/1‑138 (MQ=255)

                                                                                    aGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttctt                                                  >  7:165052/1‑138 (MQ=255)

                                                                                    aGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttctt                                                  >  1:374100/1‑138 (MQ=255)

                                                                                    aGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTGGTGTTTTCTGCATTttctt                                                  >  1:225424/1‑138 (MQ=255)

                                                                                    aGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTGTTTTTTAGTTATCAGAAGCCGTTGTGTTTTCTGGATTttctt                                                  >  6:156974/1‑138 (MQ=255)

                                                                                    aGGTTATATGTAAAAATGGTTTGAACCGAAGGTTAAGGTTTTGTAAATCAG‑TGA‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTATTTTTAATAAGACTTTGTTTTTTCTGCttttggtt                                                  >  7:174385/1‑134 (MQ=255)

                                                                                         cTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAAttt                                            >  5:405584/1‑139 (MQ=255)

                                                                                         cTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAAttt                                            >  8:221244/1‑139 (MQ=255)

                                                                                         cTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAAttt                                            >  7:523091/1‑139 (MQ=255)

                                                                                         cTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAAttt                                            >  7:470070/1‑139 (MQ=255)

                                                                                         cTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAAttt                                            >  3:197477/1‑139 (MQ=255)

                                                                                         cTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAAttt                                            >  7:155933/1‑139 (MQ=255)

                                                                                         cTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGAATTTTCTTCAAttt                                            >  3:104836/1‑139 (MQ=255)

                                                                                         cTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTATGCATTTTCTTCAAttt                                            >  5:196725/1‑139 (MQ=255)

                                                                                         cTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATAAGAAGACGTTGTGTTTTCTGCATTTTCTTAAAttt                                            >  7:202688/1‑139 (MQ=255)

                                                                                         cTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATAAGAAGACGTTGTGTTTTCTGAATTTTCTTCAAttt                                            >  3:402313/1‑139 (MQ=255)

                                                                                          tATTTAAAACGGGTTTCAACCTATTTTTAAGTTTTTTAAAATCTGTTGT‑T‑T‑TTTT‑T‑TTAA‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAAttt                                            <  5:4330/138‑1 (MQ=255)

                                                                                          tATGTAAAATGGGTTTGAACGTAATTTTATGGTTTTGTAAATCAG‑GGCTT‑T‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAAttt                                            <  6:196725/138‑1 (MQ=255)

                                                                                           aTGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTg                                          >  7:52859/1‑139 (MQ=255)

                                                                                           aTGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTg                                          >  4:390649/1‑139 (MQ=255)

                                                                                           aTGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTg                                          >  7:244371/1‑139 (MQ=255)

                                                                                            tGTAATAAGGTTTTTAACCTAATGTTAAGTTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑TTTT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAAttt                                            <  8:290957/136‑1 (MQ=255)

                                                                                               aaactgggTTTCAACGTATTGTTACGGTTTTTTAAATCCG‑TGCTT‑T‑TTTT‑T‑TTAA‑AAAAAACACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCtt                                      <  1:510830/134‑1 (MQ=255)

                                                                                                aaaaGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCttt                                     >  5:37245/1‑139 (MQ=255)

                                                                                                aaaaGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCtt                                      >  3:482241/1‑138 (MQ=255)

                                                                                                 aatgggTTTGAACCGATTGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑TTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTa                                    <  2:374100/136‑1 (MQ=255)

                                                                                                 aatgggTTTGAACCGAATGTAAAGGTTTTGTAAATCTG‑TGG‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTa                                    <  6:127640/136‑1 (MQ=255)

                                                                                                 aaaGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTATTTa                                    >  7:45929/1‑139 (MQ=255)

                                                                                                        ttGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTTTTCtttt                             >  1:503662/1‑139 (MQ=255)

                                                                                                        ttGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCtttt                             >  5:83364/1‑139 (MQ=255)

                                                                                                           aaCCTAATGTAATGTTTTTGAAAATGCG‑TCC‑GTT‑TTTT‑T‑CAAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGATGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCt                          <  4:537310/139‑1 (MQ=255)

                                                                                                           aaCAGAATGTTAATTTTTTTTAATTTAG‑TGCTT‑T‑TTTT‑T‑TTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGc                           <  1:34834/138‑1 (MQ=255)

                                                                                                            acctattGTTATGGTTTTGTAAATCTG‑TGC‑GTT‑TTTT‑T‑TTAA‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTg                         <  4:482241/133‑1 (MQ=255)

                                                                                                            aCCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTg                         <  5:130764/139‑1 (MQ=255)

                                                                                                            aCCCAATGTTATGTTTTTTTAACTCAG‑TGCTT‑T‑TTTT‑T‑TTAA‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCt                          <  4:104836/138‑1 (MQ=255)

                                                                                                              ctatggTTAAGTTTTTTTAAATCTT‑TGC‑G‑TGTTTT‑T‑TTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAg                       <  6:210677/134‑1 (MQ=255)

                                                                                                               gAATGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTTGTTATAAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAg                       >  8:533827/1‑138 (MQ=255)

                                                                                                                aaTGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTa                     >  6:524738/1‑139 (MQ=255)

                                                                                                                 aTGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTAc                    <  8:244371/139‑1 (MQ=255)

                                                                                                                 aTGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAA‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTAc                    <  8:327249/139‑1 (MQ=255)

                                                                                                                 aTGTTAAGGTTTTGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GAAT‑AAAAAAAACTCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTAc                    <  8:317840/139‑1 (MQ=255)

                                                                                                                  tGTTAGGGTTTTTTAAATCAG‑GGG‑GTT‑TTTT‑T‑GTAA‑AAAAAACACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTAc                    <  2:139409/138‑1 (MQ=255)

                                                                                                                  gtttAAGTTTTTTTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTAc                    <  8:315867/136‑1 (MQ=255)

                                                                                                                            ttGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACCTTCTTTGTcc         >  3:442186/1‑139 (MQ=255)

                                                                                                                            ttGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACCTTCTTTGTcc         >  6:291408/1‑139 (MQ=255)

                                                                                                                            ttGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACCTTCTTTGTcc         >  4:157688/1‑139 (MQ=255)

                                                                                                                            ttGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACCTTCTTTGTc          <  5:524738/138‑1 (MQ=255)

                                                                                                                            ttGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACCTTCTTTGTc          <  6:87864/138‑1 (MQ=255)

                                                                                                                            ttGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAA‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACCTTCTTTGTc          <  8:22084/138‑1 (MQ=255)

                                                                                                                             tGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTTCTGAGTACCTTCTTTGTGCt        >  1:339838/1‑139 (MQ=255)

                                                                                                                             tGTAAATCAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATAAGATGACGTTTTGTTTTCTGAATTTTGTTCAATTTTGTGTTTTTTATTTTGCTGAGTACATACTTTGTCCt        >  1:454491/1‑139 (MQ=255)

                                                                                                                                    cAG‑TGC‑GTT‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCGTTTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACCTTCTTTGTCCTGCTTCg  >  8:16286/1‑139 (MQ=255)

AAGCATAAGCACCAATTTGTTTTTTTTCATTTCCTGAATTCCCCCTGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAATCAG‑TGC‑G‑T‑TTTT‑T‑GTAT‑AAAAAAAACGCCCACATCATGTGAGCG‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACCTTCTTTGTCCTGCTTCG  >  BS168_reference_genome/2581588‑2581856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A