Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A21 F66 I1 R1	35	99.0	4347662	95.6%	4156364	127.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	2,480,667	Δ1 bp	intergenic (+42/+83)	zwf → / ← gndA	glucose‑6‑phosphate 1‑dehydrogenase (NADP‑dependent)/NADP+‑dependent 6‑P‑gluconate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	2,480,667	0	T	.	99.1%	493.8 / ‑1.7	109	intergenic (+42/+83)	zwf/gndA	glucose‑6‑phosphate 1‑dehydrogenase (NADP‑dependent)/NADP+‑dependent 6‑P‑gluconate dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base . (51/57); minor base C (0/1); total (51/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.62e-01

Only 100 of 109 total aligned reads displayed.

TATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAATAGTTTACTAAGCATTTTCGGTCTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGCTCA  >  BS168_reference_genome/2480537‑2480798

tATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACg                                                                                                                           >  3:57029/1‑139 (MQ=255)

 aactcCAAACTACGAATCAGGCTCAATGGGACCGAAATAATATGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACg                                                                                                                           <  5:66143/136‑1 (MQ=255)

 aTCTCCTTACAACGAATCAGGCTCAATGGGACCGTAATAATCTGCTGATCTTTTGGTGAAAGACGGCTAACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACg                                                                                                                           <  5:252564/138‑1 (MQ=255)

 aTCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACga                                                                                                                          >  1:201533/1‑138 (MQ=255)

 aTCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACg                                                                                                                           <  1:399903/138‑1 (MQ=255)

 aTCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACg                                                                                                                           <  7:448146/138‑1 (MQ=255)

  tctcCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGGCTTTTTTTACg                                                                                                                           <  5:543269/138‑1 (MQ=255)

      cTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTTGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCAGTTTCGG‑CTTTTTTACGCTGAAc                                                                                                                     <  7:137549/139‑1 (MQ=255)

       tAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAAc                                                                                                                     >  3:256933/1‑138 (MQ=255)

        aacaacCAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaa                                                                                                                   <  1:270217/139‑1 (MQ=255)

        aaCTACGAATCATGATCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGTAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaa                                                                                                                   <  7:234246/139‑1 (MQ=255)

        aaCTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaa                                                                                                                   <  8:130902/139‑1 (MQ=255)

        aaCTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACa                                                                                                                    >  8:438244/1‑138 (MQ=255)

        aaCTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACa                                                                                                                    >  4:54377/1‑138 (MQ=255)

        aaCTACGAATCAGGCTCAATGGGACCGAAAGAATCAGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACa                                                                                                                    >  6:376821/1‑138 (MQ=255)

        aaCTACGAATACGGCTCAATGGCCCCGAATTAATCTGTGGTTCTTTTGTTGAAAGACGGATTACACTGGTGGAACATATAATAATAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaa                                                                                                                   <  2:237653/139‑1 (MQ=255)

         aCTACGAATGAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAATAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaa                                                                                                                   <  7:348350/138‑1 (MQ=255)

         aCTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaa                                                                                                                   <  3:492732/138‑1 (MQ=255)

         aCTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaa                                                                                                                   <  6:261975/138‑1 (MQ=255)

                aTCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATaa                                                                                                           >  2:354546/1‑139 (MQ=255)

                    ggCTCAATGGGCCCGAACGAAGCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAAt                                                                                                       <  4:69889/139‑1 (MQ=255)

                    ggCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTTAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAAt                                                                                                       <  2:8755/139‑1 (MQ=255)

                     gCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAATAAGAAAAAATCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAAAAAAGGTATAACAAtc                                                                                                      <  7:312508/139‑1 (MQ=255)

                     gCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAAtc                                                                                                      >  8:195808/1‑139 (MQ=255)

                     gCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAAt                                                                                                       >  3:86334/1‑138 (MQ=255)

                          aTGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCt                                                                                                  >  8:255111/1‑138 (MQ=255)

                          aTGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCt                                                                                                  >  3:110913/1‑138 (MQ=255)

                               aCCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAAc                                                                                                                     <  5:409859/114‑1 (MQ=255)

                               aCCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAAc                                                                                                                     >  6:409859/1‑114 (MQ=255)

                               aCCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTtaataa                                                                                            >  5:161374/1‑139 (MQ=255)

                               aCCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTtaataa                                                                                            >  7:293050/1‑139 (MQ=255)

                                  caaaGAATCTGATTATCTTTTGGTGAAAGACGGCTTACACTGGTTGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACaa                                                                                         <  2:524497/138‑1 (MQ=255)

                                   aaaGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACg                                                                                                                           <  1:460551/104‑1 (MQ=255)

                                   aaaGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACg                                                                                                                           >  2:460551/1‑104 (MQ=255)

                                   aaaGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACaaa                                                                                        <  5:384374/139‑1 (MQ=255)

                                   aaaGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACaa                                                                                         >  8:11692/1‑138 (MQ=255)

                                         tCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAAcccc                                                                                   >  2:6562/1‑138 (MQ=255)

                                             atgatCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCa                                                                                 <  1:58582/136‑1 (MQ=255)

                                             atgatCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCa                                                                                 <  3:268632/136‑1 (MQ=255)

                                                     ttGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAagag                                                                      >  2:394768/1‑139 (MQ=255)

                                                     ttGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAag                                                                        <  1:234653/137‑1 (MQ=255)

                                                     ttGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAag                                                                        <  5:376821/137‑1 (MQ=255)

                                                     ttGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAag                                                                        <  8:437245/137‑1 (MQ=255)

                                                            aaGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCggg                                                               >  7:464409/1‑139 (MQ=255)

                                                                cGGCTTACAGTGGTGGAACATATAATATGAATAAAAAAGCCGAAATTGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAATGTATTACAATCTCCTTAATAACAAAACCCCCTAAGCTTAAGAGCTTCGGGGGt                                                            <  4:217699/138‑1 (MQ=255)

                                                                   cTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTc                                                         >  2:125433/1‑138 (MQ=255)

                                                                   cTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTc                                                         >  1:67945/1‑138 (MQ=255)

                                                                       cacTGGTGGAACATATAATAAGAAGAAATAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTg                                                    <  2:519488/139‑1 (MQ=255)

                                                                       cacTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTAtt                                                     <  6:505652/138‑1 (MQ=255)

                                                                            gtggATCATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCAGTTTCGG‑CTTTTTTTCGCTGAACAAAGGTATAACAAGCTCCTTAATTACAAAACCCCCAACGCTTATGGGCTTCGGGGGTGTCTAGTGATGAc                                               >  4:116881/1‑139 (MQ=255)

                                                                            gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGa                                                   <  6:382478/135‑1 (MQ=255)

                                                                            gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTAc                                               >  6:308956/1‑139 (MQ=255)

                                                                            gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTAc                                               >  6:126990/1‑139 (MQ=255)

                                                                            gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTAc                                               >  6:153499/1‑139 (MQ=255)

                                                                            gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTAc                                               >  6:375819/1‑139 (MQ=255)

                                                                            gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTAc                                               >  5:225017/1‑139 (MQ=255)

                                                                            gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTAc                                               <  5:149064/139‑1 (MQ=255)

                                                                            gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTAc                                               >  6:545868/1‑139 (MQ=255)

                                                                            gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTAc                                               <  4:110913/139‑1 (MQ=255)

                                                                            gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTAc                                               >  3:504515/1‑139 (MQ=255)

                                                                            gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTAc                                               >  7:352720/1‑139 (MQ=255)

                                                                            gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTAc                                               >  1:452497/1‑139 (MQ=255)

                                                                            gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTAc                                               >  1:504086/1‑139 (MQ=255)

                                                                             tggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAAt                                                                                                       >  8:491244/1‑82 (MQ=255)

                                                                             tggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACt                                              >  5:458242/1‑139 (MQ=255)

                                                                             tggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACt                                              >  3:74762/1‑139 (MQ=255)

                                                                               gAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACtt                                             <  2:201533/138‑1 (MQ=255)

                                                                                 aCATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCAt                                          <  8:195280/139‑1 (MQ=255)

                                                                                 aCATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCAt                                          >  7:284656/1‑139 (MQ=255)

                                                                                  cATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATc                                         >  3:169343/1‑139 (MQ=255)

                                                                                    tataATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCa                                       >  5:12834/1‑139 (MQ=255)

                                                                                    tataATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCa                                       >  2:202781/1‑139 (MQ=255)

                                                                                    tataATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCa                                       <  4:256933/139‑1 (MQ=255)

                                                                                    tataATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTAGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATcaa                                       >  2:161042/1‑137 (MQ=255)

                                                                                      taataaGATGATAAAAGACGAAAATGTTTAC‑AAGGATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt                                     <  8:223416/139‑1 (MQ=255)

                                                                                      taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt                                     <  1:202781/139‑1 (MQ=255)

                                                                                      taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt                                     <  2:214122/139‑1 (MQ=255)

                                                                                      taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt                                     <  3:191365/139‑1 (MQ=255)

                                                                                      taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt                                     <  7:255111/139‑1 (MQ=255)

                                                                                      taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt                                     <  7:232694/139‑1 (MQ=255)

                                                                                      taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt                                     <  5:375819/139‑1 (MQ=255)

                                                                                      taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt                                     <  5:126990/139‑1 (MQ=255)

                                                                                      taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt                                     <  6:458242/139‑1 (MQ=255)

                                                                                       aataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACATAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt                                     <  7:438244/138‑1 (MQ=255)

                                                                                       aataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt                                     >  2:301455/1‑138 (MQ=255)

                                                                                        ataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCa                                   <  3:116881/139‑1 (MQ=255)

                                                                                        ataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCa                                   <  1:354546/139‑1 (MQ=255)

                                                                                           agaagaAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTa                                <  4:169343/139‑1 (MQ=255)

                                                                                            gaagaaAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCGTTAATAACAAAACCCCCAAAGCTGAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAGTCAGTAt                               >  6:520825/1‑139 (MQ=255)

                                                                                             aagaaAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGttt                                                          >  2:349832/1‑111 (MQ=255)

                                                                                             aagaaAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGttt                                                          <  1:349832/111‑1 (MQ=255)

                                                                                                aaaaaaGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATgg                             >  8:387408/1‑137 (MQ=255)

                                                                                                                    acaaGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGt         <  4:74762/136‑1 (MQ=255)

                                                                                                                    acaaGCATTTTAGG‑CTTTTTTACGATGAACAAATGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGt         <  8:464409/136‑1 (MQ=255)

                                                                                                                     caaGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTa        <  1:394768/137‑1 (MQ=255)

                                                                                                                      aaGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACg      <  6:225017/139‑1 (MQ=255)

                                                                                                                      aaGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACg      <  7:195808/139‑1 (MQ=255)

                                                                                                                         cATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTAc       >  5:384440/1‑135 (MQ=255)

                                                                                                                         cATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTAc       <  6:384440/135‑1 (MQ=255)

                                                                                                                           ttttCGG‑CTTTTTTACGCTGAACAAAGCTAAAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCATTCTTTGTCAGTACGCTCa  >  4:252409/1‑138 (MQ=255)

TATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAATAGTTTACTAAGCATTTTCGGTCTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGCTCA  >  BS168_reference_genome/2480537‑2480798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A