Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F67 I0 R1 253 76.5 2897616 95.8% 2775916 135.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,178,209 C→T 37.2% R978C (CGT→TGT)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,178,2090CT37.2% 37.3 / 85.6 84R978C (CGT→TGT) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base C (14/22);  new base T (14/14);  total (42/42)
Fisher's exact test for biased strand distribution p-value = 4.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTGGGACCCGCACA  >  CP009273/4178073‑4178347
                                                                                                                                        |                                                                                                                                          
gCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTa                                                                                                                                         >  1:1189692/1‑140 (MQ=255)
gCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTa                                                                                                                                         >  2:100272/1‑140 (MQ=255)
gCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTa                                                                                                                                         >  1:876316/1‑140 (MQ=255)
    aCGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAACCTGGTTATCACTTCCTGTAATac                                                                                                                                     <  2:354261/140‑1 (MQ=255)
    aCGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATac                                                                                                                                     <  2:778724/140‑1 (MQ=255)
       ttCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTGGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATactga                                                                                                                                  >  2:699919/1‑140 (MQ=255)
       ttCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTCAACTGGTTATCACTTCCAGTAATactga                                                                                                                                  >  2:681311/1‑140 (MQ=255)
       ttCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATactga                                                                                                                                  >  2:489035/1‑140 (MQ=255)
       ttCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATactga                                                                                                                                  >  2:111416/1‑140 (MQ=255)
       ttCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCAGTCATactga                                                                                                                                  >  2:1358844/1‑140 (MQ=255)
       ttCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCAGTAATactga                                                                                                                                  >  2:621144/1‑140 (MQ=255)
       ttCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCAGTAATactga                                                                                                                                  >  2:1085176/1‑140 (MQ=255)
        tCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATactgaa                                                                                                                                 <  1:146558/140‑1 (MQ=255)
            caTCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATactgaactga                                                                                                                             <  2:768437/140‑1 (MQ=255)
                ggtggtGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGaactgaaact                                                                                                                         >  2:946626/1‑140 (MQ=255)
                 gtggtgCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTACTACTGaactgaaactg                                                                                                                        >  1:104463/1‑140 (MQ=255)
                      gCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTCGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCCGCGGTCAACTGGTTATCACTTCCCGGAATACTGCCCTGAAACTGATcgc                                                                                                                   >  1:324545/1‑139 (MQ=255)
                      gCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATcga                                                                                                                   >  1:27163/1‑140 (MQ=255)
                       cGGCATCTCGTGCGGCTGCTGAAGCCAGCATCCAAGGGAAAACCAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGACCTCCAGCGGTAAACTGGTTCTGACTTCCTGTCATACGGTACTGAAACTGATcgac                                                                                                                  >  2:985949/1‑140 (MQ=255)
                            tctcGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAAtt                                                                                                             >  2:1200415/1‑140 (MQ=255)
                               cGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCgg                                                                                                          <  1:100272/140‑1 (MQ=255)
                                 tGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTc                                                                                                        <  1:1407451/140‑1 (MQ=255)
                                    ggCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTa                                                                                                     >  2:563884/1‑140 (MQ=255)
                                              tCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTaaagaaag                                                                                           <  2:1437673/140‑1 (MQ=255)
                                              tCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTaaagaaag                                                                                           <  2:139607/140‑1 (MQ=255)
                                               ccAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGc                                                                                          <  2:134925/140‑1 (MQ=255)
                                               ccAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGc                                                                                          <  2:651506/140‑1 (MQ=255)
                                               ccAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGc                                                                                          <  2:265497/140‑1 (MQ=255)
                                                   cATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAACCTGGTTATCACTTCCAGTAATACTGAACTGAAACTGATCGACGACTTCGGTCGTAATAAAGATAGCTACa                                                                                      >  2:364317/1‑140 (MQ=255)
                                                   cATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACa                                                                                      <  1:80460/140‑1 (MQ=255)
                                                   cATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCAGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACa                                                                                      >  1:253666/1‑140 (MQ=255)
                                                   cATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCAGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACa                                                                                      >  1:94199/1‑140 (MQ=255)
                                                     tCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACaaa                                                                                    <  2:1003096/140‑1 (MQ=255)
                                                     tCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTGCCCGTAAGACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACaaa                                                                                    <  1:825673/140‑1 (MQ=255)
                                                        aaGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTa                                                                                 >  1:941559/1‑140 (MQ=255)
                                                        aaGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTa                                                                                 >  1:239509/1‑140 (MQ=255)
                                                        aaGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTa                                                                                 >  2:1194803/1‑140 (MQ=255)
                                                          gTGAAAAACAATGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGAGGTAAACTGTTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAACGAAAGCTACAAAGTAcc                                                                               <  2:723531/140‑1 (MQ=255)
                                                          gTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTAcc                                                                               >  2:881289/1‑140 (MQ=255)
                                                             aaaaaCAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTa                                                                            <  1:1401407/140‑1 (MQ=255)
                                                                  cAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTg                                                                       <  2:1044639/140‑1 (MQ=255)
                                                                  cAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTg                                                                       <  1:133447/140‑1 (MQ=255)
                                                                  cAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTg                                                                       <  2:1273715/140‑1 (MQ=255)
                                                                     aGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCAGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCgg                                                                    >  1:810840/1‑140 (MQ=255)
                                                                     aGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCAGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCgg                                                                    >  1:1312213/1‑140 (MQ=255)
                                                                     aGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCAGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCgg                                                                    >  1:810838/1‑140 (MQ=255)
                                                                            aTCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGc                                                             >  1:1123238/1‑140 (MQ=255)
                                                                                aGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGaaa                                                         >  1:758120/1‑140 (MQ=255)
                                                                                  cTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAgg                                                       <  2:927276/140‑1 (MQ=255)
                                                                                  cTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAgg                                                       <  2:259770/140‑1 (MQ=255)
                                                                                  cTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAgg                                                       <  2:230167/140‑1 (MQ=255)
                                                                                   tCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGc                                                      <  1:996890/140‑1 (MQ=255)
                                                                                   tCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGc                                                      <  1:996849/140‑1 (MQ=255)
                                                                                    cAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCg                                                     <  2:871700/140‑1 (MQ=255)
                                                                                    cAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCg                                                     <  2:758120/140‑1 (MQ=255)
                                                                                     aGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCAGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGa                                                    <  2:1312213/140‑1 (MQ=255)
                                                                                     aGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCAGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGa                                                    <  2:810840/140‑1 (MQ=255)
                                                                                     aGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCAGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGa                                                    <  2:810838/140‑1 (MQ=255)
                                                                                      gCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCAGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGAt                                                   >  2:459158/1‑140 (MQ=255)
                                                                                         aCGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTCATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGGACCTTACGGTGCGGTACTGGCGAAAGGCGATGGc                                                <  1:316786/140‑1 (MQ=255)
                                                                                         aCGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGc                                                <  1:727000/140‑1 (MQ=255)
                                                                                            tGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGaa                                             >  2:662034/1‑140 (MQ=255)
                                                                                            tGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGaa                                             >  2:1245501/1‑140 (MQ=255)
                                                                                            tGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGaa                                             >  2:455360/1‑140 (MQ=255)
                                                                                            tGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGaa                                             >  2:146968/1‑140 (MQ=255)
                                                                                             gAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAAc                                            <  1:162977/140‑1 (MQ=255)
                                                                                                     ttGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCt                                    <  2:239509/140‑1 (MQ=255)
                                                                                                          taCTCCAGCGGTTAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATAGCCGAATTCGGGCCTACGAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGACCAGGTTGCTggcgg                               >  2:99448/2‑140 (MQ=255)
                                                                                                               cAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGaaa                          >  1:32338/1‑140 (MQ=255)
                                                                                                               cAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGaaa                          >  1:32211/1‑140 (MQ=255)
                                                                                                               cAGCGGTAAACTGGTTATCACTTCCAGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGaaa                          >  1:755347/1‑140 (MQ=255)
                                                                                                               cAGCGGTAAACTGGTTATCACTTCCAGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGaaa                          >  1:914599/1‑140 (MQ=255)
                                                                                                               cAGCGGTAAACTGGTTATCACTTCCAGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGaaa                          >  1:467149/1‑140 (MQ=255)
                                                                                                                      aaaCTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGc                   <  2:1302998/140‑1 (MQ=255)
                                                                                                                      aaaCTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGc                   <  2:21493/140‑1 (MQ=255)
                                                                                                                      aaaCTGGTTATCACTTCCAGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGc                   <  2:391218/140‑1 (MQ=255)
                                                                                                                       aaCTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCa                  <  1:1078966/140‑1 (MQ=255)
                                                                                                                           ggTTATCACTTCCAGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACt              <  1:913425/140‑1 (MQ=255)
                                                                                                                           ggTTATCACTTCCAGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACt              <  1:1207253/140‑1 (MQ=255)
                                                                                                                            gTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTg             >  1:1346172/1‑140 (MQ=255)
                                                                                                                             ttATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTgg            <  1:252644/140‑1 (MQ=255)
                                                                                                                             ttATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTgg            <  1:450408/140‑1 (MQ=255)
                                                                                                                               aTCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTGGGa          >  2:321321/1‑140 (MQ=255)
                                                                                                                                     tCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGACAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTGGGACCCGca    <  2:773437/140‑1 (MQ=255)
                                                                                                                                       ccGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTGGGACCCGcaca  <  1:111416/140‑1 (MQ=255)
                                                                                                                                       ccGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTGGGACCCGcaca  <  1:489035/140‑1 (MQ=255)
                                                                                                                                       ccGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTGGGACCCGcaca  <  1:699919/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                          
GCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTGGGACCCGCACA  >  CP009273/4178073‑4178347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A