Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F22 I1 R1 1 91.7 3230112 98.4% 3178430 134.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 4,178,209 C→T R978C (CGT→TGT)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,178,2090CT97.5% 298.5 / ‑2.5 79R978C (CGT→TGT) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (33/44);  total (34/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTGGGACCCGCA  >  CP009273/4178073‑4178345
                                                                                                                                        |                                                                                                                                        
gCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAAGGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTa                                                                                                                                       >  2:325221/1‑140 (MQ=255)
gCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTa                                                                                                                                       >  1:1030322/1‑140 (MQ=255)
gCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTa                                                                                                                                       >  1:37416/1‑140 (MQ=255)
  gTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAAt                                                                                                                                     >  1:288195/1‑140 (MQ=255)
    aCGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATac                                                                                                                                   <  1:977358/140‑1 (MQ=255)
     cGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATact                                                                                                                                  >  1:1280042/1‑140 (MQ=255)
       ttCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCAAAGTGAAAAACAAAGGTAGCATCAATCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATactga                                                                                                                                >  2:1480960/1‑140 (MQ=255)
                ggtggtGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGaactgaaact                                                                                                                       >  2:1426242/1‑140 (MQ=255)
                     tgCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATcg                                                                                                                  <  1:547228/140‑1 (MQ=255)
                         gCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATcgacga                                                                                                              >  1:1170051/1‑140 (MQ=255)
                          cATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGaa                                                                                                             <  2:1393304/140‑1 (MQ=255)
                               cGTGCGGCTGGTGAATACATCATACAAGTGAACAAAAAAGGTATCATCAAGCTCAGGAACGTGAAGTCGGTTGTGAACTACAGCGGTAAACTGGTTATCACTTCCTGTCATACTGAACTGAAAATGATCGACGAATTCgg                                                                                                        <  1:325221/140‑1 (MQ=255)
                               cGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCgg                                                                                                        >  2:1426810/1‑140 (MQ=255)
                               cGTGCGGCTGCTGAATCCAGCATACAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCgg                                                                                                        <  2:214942/140‑1 (MQ=255)
                               cGTGCGACTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCgg                                                                                                        <  2:295764/140‑1 (MQ=255)
                                   cGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGt                                                                                                    >  1:1280108/1‑140 (MQ=255)
                                    ggCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTa                                                                                                   >  2:178399/1‑140 (MQ=255)
                                           gAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTaaaga                                                                                            <  1:1606672/140‑1 (MQ=255)
                                            aaTCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTaaagaa                                                                                           <  1:54047/140‑1 (MQ=255)
                                              tCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTaaagaaag                                                                                         <  2:344870/140‑1 (MQ=255)
                                              tCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTaaagaaag                                                                                         <  2:5957/140‑1 (MQ=255)
                                              tCCAGCATACAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTaaagaaag                                                                                         <  2:5936/140‑1 (MQ=255)
                                                cAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGACAGCt                                                                                       >  1:1378734/1‑140 (MQ=255)
                                                 aGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTa                                                                                      <  2:30991/140‑1 (MQ=255)
                                                 aGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTa                                                                                      >  1:1016047/1‑140 (MQ=255)
                                                   cATCCAAGTGAAAAACAAAGGTAGCATGAAGCTCAGCATCGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACa                                                                                    <  2:938217/140‑1 (MQ=255)
                                                   cATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACa                                                                                    <  2:1177642/140‑1 (MQ=255)
                                                   cATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACa                                                                                    >  1:530607/1‑140 (MQ=255)
                                                   cATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACa                                                                                    <  1:348549/140‑1 (MQ=255)
                                                     tCCAAGTGACAAACAAAGGTAGCATCAACCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGTTAAACTGGTTATCACTTCCTGTAATACTGACCTGTAACTGATCGACGAATTCGGTCGTACTAACGAAAGCTACaca                                                                                  <  2:310417/140‑3 (MQ=255)
                                                     tCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTTAAGAAAGCTACaaa                                                                                  <  2:650521/140‑1 (MQ=255)
                                                     tCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACaaa                                                                                  <  2:1280042/140‑1 (MQ=255)
                                                     tCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACaaa                                                                                  <  2:1016047/140‑1 (MQ=255)
                                                     tCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACaaa                                                                                  <  2:1428793/140‑1 (MQ=255)
                                                        aaGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTa                                                                               >  1:409502/1‑140 (MQ=255)
                                                             aaaaaCAAAGGTAGCATCACGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTa                                                                          <  1:905288/140‑1 (MQ=255)
                                                             aaaaaCAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTACTACTTAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTa                                                                          <  1:501405/140‑1 (MQ=255)
                                                             aaaaaCAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGACACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTa                                                                          <  1:953406/140‑1 (MQ=255)
                                                             aaaaaCAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTa                                                                          <  1:1426810/140‑1 (MQ=255)
                                                             aaaaaCAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTa                                                                          <  2:1013132/140‑1 (MQ=255)
                                                             aaaaaCAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTa                                                                          <  2:881288/140‑1 (MQ=255)
                                                             aaaaaCAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTa                                                                          <  2:1303032/140‑1 (MQ=255)
                                                             aaaaaCAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATAATGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTa                                                                          <  2:743795/140‑1 (MQ=255)
                                                             aaaaaCAAAGGTACCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGAAGAATTCGGTCGTACTAAAGACAGCTACAAAGTACCTTa                                                                          <  1:542168/140‑1 (MQ=255)
                                                                  cAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTg                                                                     <  2:37416/140‑1 (MQ=255)
                                                                  cAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTg                                                                     <  2:1030322/140‑1 (MQ=255)
                                                                  cAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTg                                                                     <  1:30857/140‑1 (MQ=255)
                                                                    aaGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCg                                                                   >  2:170433/1‑140 (MQ=255)
                                                                     aGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGACCTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCgg                                                                  >  1:571470/1‑140 (MQ=255)
                                                                                aGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCCCTTCCTGTAATACTGAACTGCAACTGATCGCCGAATTCGGTCGTACTAAAGAAAGCTCCAAAGTACGTTACGGTGCGGGCCTGGCGaag                                                       >  1:12994/1‑139 (MQ=255)
                                                                                 gCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAg                                                      >  1:1427970/1‑140 (MQ=255)
                                                                                 gCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAg                                                      >  1:908564/1‑140 (MQ=255)
                                                                                          cGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACa                                                                                    >  2:1061827/1‑101 (MQ=255)
                                                                                          cGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACa                                                                                    <  1:1061827/101‑1 (MQ=255)
                                                                                          cGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCg                                             >  1:1495471/1‑140 (MQ=255)
                                                                                            tGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGaa                                           >  1:1283707/1‑140 (MQ=255)
                                                                                            tGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGaa                                           >  1:495932/1‑140 (MQ=255)
                                                                                            tGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGaa                                           >  1:346753/1‑140 (MQ=255)
                                                                                               aGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGACAGGCGATGGCGAACAg                                        >  1:776821/1‑140 (MQ=255)
                                                                                                               cAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGaac                        >  1:1555058/1‑139 (MQ=255)
                                                                                                               cAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGaaa                        >  1:292006/1‑140 (MQ=255)
                                                                                                               cAGCGGTAAACTGGTTATCACTTCCTGGAATACTGAACTGAGACTGATCGACGAATTCGGTGGTACGAATGAAAGCTACAAAGTACCTTACGGTGCGGTACTGCCGAAAGGCGATGGCGAACAGGTTGCTGGCGGAGaag                        >  2:386832/1‑139 (MQ=255)
                                                                                                                    gTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGtt                   <  1:645146/140‑1 (MQ=255)
                                                                                                                      aaaCTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGc                 <  2:1378734/140‑1 (MQ=255)
                                                                                                                       aaCTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGCACAGGTTGCTGGCGGCGAAACCGTTGCa                >  2:777094/1‑140 (MQ=255)
                                                                                                                            gTTCTCCCTGCCTGTAATACTGCCCTGAACCTGCTCGACGAATTCGGTCGTCCTAAAGAAAGCTCCAAAGTACCTTACGGTGCGGTACTGGCGCAAGGCGATGGCGACCAGGTTGCTGGCGGCGACACCGTGGCACACTg           >  2:1460907/1‑140 (MQ=255)
                                                                                                                            gTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTg           >  1:581173/1‑140 (MQ=255)
                                                                                                                            gTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTg           >  1:1284498/1‑140 (MQ=255)
                                                                                                                             ttATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTgg          <  2:1170051/140‑1 (MQ=255)
                                                                                                                             ttATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTgg          <  2:801735/140‑1 (MQ=255)
                                                                                                                              tATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTggg         <  2:1427970/140‑1 (MQ=255)
                                                                                                                              tATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTggg         <  2:571470/140‑1 (MQ=255)
                                                                                                                              tATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTggg         <  2:908564/140‑1 (MQ=255)
                                                                                                                               aTCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTGGGa        >  1:924738/1‑140 (MQ=255)
                                                                                                                                tCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTGGGAc       <  2:1495471/140‑1 (MQ=255)
                                                                                                                                   cTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTGGGACCCg    <  1:200684/140‑1 (MQ=255)
                                                                                                                                   cTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTGGGACCCg    <  1:941691/140‑1 (MQ=255)
                                                                                                                                     tCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTGGGACCCGca  <  2:770167/140‑1 (MQ=255)
                                                                                                                                     tCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTGGGACCCGca  <  2:915463/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
GCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTGGGACCCGCA  >  CP009273/4178073‑4178345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A