Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A16 F19 I0 R1 32 84.8 2851444 95.0% 2708871 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_pae_tpiA 1,544,022 C→A 100% L13F (TTG→TTT)  narU ← nitrate/nitrite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA1,544,0220CA100.0% 239.5 / NA 74L13F (TTG→TTT) narUnitrate/nitrite transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (35/39);  total (35/39)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑C‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAA  >  NC_000913_3_pae_tpiA/1543879‑1544157
                                                                                                                                                 |                                                                                                                                          
ttGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑A‑a‑aaaa                                                                                                                                     >  1:851144/1‑148 (MQ=255)
ttGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGAAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑A‑a‑aaaa                                                                                                                                     <  1:110136/149‑2 (MQ=255)
 tGAGATTAACGGTAACTGCGCTAAATATCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑A‑a‑aaaag                                                                                                                                    <  1:179891/149‑3 (MQ=255)
 tGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑A‑a‑aaaag                                                                                                                                    <  1:180247/149‑3 (MQ=255)
  gagaTTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATATTTTCATTTATTTTCCTAGAAGACAGGATATTCTTATATTCAGTCG‑CG‑A‑a‑aaaaca                                                                                                                                   >  2:428768/1‑146 (MQ=255)
   agaTTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCACAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑A‑a‑aaaagat                                                                                                                                  >  1:923045/1‑145 (MQ=255)
      tcaaCGGTAACTGCGCTAAATAGCATCCACACACAGAAGGCAAGAAATAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAAc                                                                                                                                <  2:887653/147‑1 (MQ=255)
          cGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACt                                                                                                                            >  2:426672/1‑149 (MQ=255)
                cTGCGCTAAATCGCATCCAGACCCAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTAttttt                                                                                                                      <  1:384252/149‑1 (MQ=255)
                 tGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTtc                                                                                                                     >  2:1180533/1‑149 (MQ=255)
                 tGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCAAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTtc                                                                                                                     >  2:412705/1‑149 (MQ=255)
                      tAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCAtt                                                                                                                >  1:1420304/1‑149 (MQ=255)
                      tAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCAtt                                                                                                                >  1:645217/1‑149 (MQ=255)
                      tAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCAtt                                                                                                                >  1:645065/1‑149 (MQ=255)
                       aaaTAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTtctcctct                                                                                                               >  2:1109037/1‑145 (MQ=255)
                       aaaTAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCAttt                                                                                                               <  1:119670/149‑1 (MQ=255)
                        aaTAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCAtttt                                                                                                              <  1:1102009/149‑1 (MQ=255)
                          tAGCATCCAGACACAGAAGGCAAGAAGGAGACAAATGACTTATATCCAAAGATTTCTTCGAACAATATGTTTTCCTTTATTTTCCCAGAAAGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGc                                                                                                            <  2:620505/149‑1 (MQ=255)
                           aGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑AAA‑AAAGATAACGACTATTTTTCTCATTTTGCa                                                                                                           <  1:422166/149‑1 (MQ=255)
                            gCATCCAGACACAGAATGCAATAAGTAGACAACTGACTGATATCCAGAGATTCCTTCGAGCAATATGTTTCCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAg                                                                                                          <  1:1167453/149‑1 (MQ=255)
                                ccAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGGTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTGTTTTTCTCATTTTGCAGTGcc                                                                                                      >  2:366864/1‑149 (MQ=255)
                                 cAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCGTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑GGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCa                                                                                                     >  1:35163/1‑149 (MQ=255)
                                        aGAAGGCAAGAAGTAGACATCTTACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGtt                                                                                              <  2:226852/149‑1 (MQ=255)
                                        aGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGtt                                                                                              >  2:1304167/1‑149 (MQ=255)
                                        aGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGtt                                                                                              <  1:99179/149‑1 (MQ=255)
                                        aGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑AAA‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGtt                                                                                              >  1:1101704/1‑149 (MQ=255)
                                         gAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTc                                                                                             >  1:862756/1‑149 (MQ=255)
                                           aGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCt                                                                                           <  2:1383710/149‑1 (MQ=255)
                                               aagaagTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTcaca                                                                                       <  2:130468/149‑1 (MQ=255)
                                                 gaagTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATg                                                                                     <  1:721239/149‑1 (MQ=255)
                                                  aagTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGc                                                                                    >  1:349579/1‑149 (MQ=255)
                                                       gACAACTGACTGATATCCAGATATTTCTCCGAGCACTAGGTCTTCCATTATTTGCACAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑AAA‑AAAGATAACGACTATTTTTCTCCTTTTGCAGTGCCATATTATTCCTCAAATGcac‑aca                                                                              >  2:671650/1‑149 (MQ=255)
                                                       gACAACTGACTGATATCCAGAGATTTCTTGGAACCAGAGAGTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGACATATTGTTCCTCACATGtac‑cca                                                                              >  2:671694/1‑149 (MQ=255)
                                                        aCAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑AAA‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACAt                                                                             >  1:95161/1‑149 (MQ=255)
                                                          aaCTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTg                                                                           >  2:182061/1‑149 (MQ=255)
                                                          aaCTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTg                                                                           >  2:1159595/1‑149 (MQ=255)
                                                          aaCTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTg                                                                           >  1:1021711/1‑149 (MQ=255)
                                                               actgaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAAt                                                                      <  2:839389/149‑1 (MQ=255)
                                                                       ccAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑AAA‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGaaaaaaa                                                              >  2:217161/1‑149 (MQ=255)
                                                                       ccAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTGTCCACAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGaaaaaaa                                                              <  1:264734/149‑1 (MQ=255)
                                                                       ccAGAGATTTCTTCGAGCAATATGCTTTCGTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTGCCTTACATGCAC‑ACATTGGTAATGaaaaaaa                                                              <  2:1359971/149‑1 (MQ=255)
                                                                           agaTTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑AAA‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACa                                                          >  1:722291/1‑149 (MQ=255)
                                                                                   tcGAGCAATATGTTTTCCCTTCTTTGCCCAGAAGGCCTGATTTTCTGGTTTCCAGCCG‑CGAA‑A‑AAAGATAACGACTATTTCTCTCACTGTGCAGTGACATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGa                                                  <  1:631096/149‑1 (MQ=255)
                                                                                     gAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAgg                                                <  1:31092/149‑1 (MQ=255)
                                                                                      ggCAATAGGTTTTCCTTTATTGTCCCAGAAGGCCGTATTTTCTGCTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGt                                               <  2:851144/148‑1 (MQ=255)
                                                                                        gaaTTTGTTTTCCTTTATTTTTCTAGAAGGTCGGATTTTCTGGTTTCCAGTCA‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTaa                                             <  2:1209819/148‑1 (MQ=255)
                                                                                        cAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTaa                                             <  1:1166511/149‑1 (MQ=255)
                                                                                        cAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGCaa                                             >  2:687391/1‑149 (MQ=255)
                                                                                           tatGTTTTCCTTTATTTTCCCAGGAGGCCGGATTTTCGGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGgc                                          <  2:1272656/149‑1 (MQ=255)
                                                                                           gatGTATTCCTTTATTTGCCCAGGAGGCCGGATTTTCTGTTTTCCAGTCG‑TGAA‑A‑AAAGATAACGACTATTTTTGTCATTTTGCAGTGCCATATTGTTCCTCACAGTTAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGgc                                          <  2:566266/148‑1 (MQ=255)
                                                                                               ttttCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCaa                                      >  2:1215696/1‑149 (MQ=255)
                                                                                                   ccTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGc                                  <  1:795013/149‑1 (MQ=255)
                                                                                                     tttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCa                                >  1:646193/1‑149 (MQ=255)
                                                                                                          tttCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACACTGGTAATGAAAAAAAGACAAAACACGAGGAAAGGCGCAATAGCCAGttat                           >  2:911891/1‑149 (MQ=255)
                                                                                                               cAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACGTGTAT‑TGAGGTTTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCAAGTTATTAGaa                      >  1:695114/1‑149 (MQ=255)
                                                                                                               cAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGaa                      <  1:374359/149‑1 (MQ=255)
                                                                                                               cAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGaa                      >  2:931951/1‑149 (MQ=255)
                                                                                                               cAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCAATTGCATAAAAGTGGT‑ATGAAAAAAAGACAAAACACGAGGTAAGGGGCAATAGCCAGTTATTAGaa                      >  1:694702/1‑149 (MQ=255)
                                                                                                               cAGAAGGCCGGATTTTCCGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATGTTGCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGaa                      <  1:310009/149‑1 (MQ=255)
                                                                                                                   agtccGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTaa                  <  2:952800/146‑1 (MQ=255)
                                                                                                                   aGGCCGCAGTTTGTGGTTCCATGTCG‑CC‑A‑ATAAAGATAACTACTATTTTTTTAATTTTGCAGTACCATATTGTTCCTCCCATTCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTaa                  <  2:318451/149‑1 (MQ=255)
                                                                                                                    ggCCGGATTTTCTTGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAg                 <  1:683867/149‑1 (MQ=255)
                                                                                                                    ggCCGGATTTTCTGGTTTCCAGTCGCCA‑A‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAg                 <  1:176930/149‑1 (MQ=255)
                                                                                                                       cGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATCTTTTTCGTCAAATGGGT‑ACATTGGTAATGAAAAAAAGACAAAACAGGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGAt              >  1:1402052/1‑149 (MQ=255)
                                                                                                                       cGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTAAGATATTGTTCCTCAAATGTAC‑AGAGTGGTAATGAAAAAAAGACAAAACACAAGGTAAGGCGCAATAGCCAGTTCTTAGAATTAAGGAt              >  1:1401906/1‑149 (MQ=255)
                                                                                                                          aTTTTTGGGTGTTCAGTCG‑CGAA‑A‑AAAAATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGaa           <  2:91567/149‑1 (MQ=255)
                                                                                                                          aTTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGaa           <  2:236580/149‑1 (MQ=255)
                                                                                                                              tCTTGTTTCCAGTCG‑AGAA‑A‑AAACATAACGACTATTTTTCGCATTTTGCGGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTgg       <  1:964677/149‑1 (MQ=255)
                                                                                                                              tCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCACAATAGCCAGTTATTAGAATTAAGGATGAATTgg       <  2:349579/149‑1 (MQ=255)
                                                                                                                              tCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAAAACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTgg       >  1:656778/1‑149 (MQ=255)
                                                                                                                                 ggTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTg    >  1:130984/1‑149 (MQ=255)
                                                                                                                                 ggTTTCCAGTCG‑CG‑AAA‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTg    <  1:366864/149‑1 (MQ=255)
                                                                                                                                   tttCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGaa  <  1:1304167/149‑1 (MQ=255)
                                                                                                                                   tttCCAATCG‑CGAA‑A‑AAAGATAACGTCTAGTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑AAAATGGAAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGaa  <  2:778488/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                          
TTGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑C‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAA  >  NC_000913_3_pae_tpiA/1543879‑1544157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A