Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A24 F80 I1 R1 18 118.6 4166156 93.9% 3912020 141.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 1,544,022 C→A L13F (TTG→TTT)  narU ← nitrate/nitrite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA1,544,0220CA98.9% 299.2 / NA 91L13F (TTG→TTT) narUnitrate/nitrite transporter
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (49/40);  total (50/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑C‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGC  >  NC_000913_3_pae_tpiA/1543879‑1544161
                                                                                                                                                 |                                                                                                                                             
ttGAGATTAATGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑A‑aaaaa                                                                                                                                         <  1:1498569/149‑2 (MQ=255)
  gagaTTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑A‑aaaaaga                                                                                                                                       <  1:1325449/149‑4 (MQ=255)
   agaTTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑A‑aaaaagat                                                                                                                                      <  2:2005271/149‑5 (MQ=255)
    gaTTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑A‑aaaaagata                                                                                                                                     <  1:732946/149‑6 (MQ=255)
      ttAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑Ac                                                                                                                                   <  1:311915/149‑1 (MQ=255)
                   cgcTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTtctc                                                                                                                      <  2:289634/149‑1 (MQ=255)
                   cgcTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑A‑aaaaa                                                                                                                                         <  2:1899377/130‑2 (MQ=255)
                   cgcTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑A‑aaaaa                                                                                                                                         >  1:1899377/1‑129 (MQ=255)
                          tAGCATCCATACACAGAAGGTAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGc                                                                                                               <  1:8418/149‑1 (MQ=255)
                          tAGCATCCAGACACAGAAGGAAAGCAGTAGACGACTAAGTGATATCCAGAGATTTCTTCGAGCAAGATGGTTTCCTTTATTGTCCCAGTAGGGCGGATTTTCTGGTTTCAAGTCG‑CG‑CAAAAAGTGA‑ACGACTATTTTTATCCGTTTGc                                                                                                               >  2:1804303/1‑149 (MQ=255)
                           aGCATCCATACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCa                                                                                                              <  2:1422662/149‑1 (MQ=255)
                           aGCATCCAAACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCa                                                                                                              <  1:251644/149‑1 (MQ=255)
                           aGCAACCAGACTCAGAAAGTAAGAAGTAGACAACTGACTCATATGCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGCAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCa                                                                                                              <  2:2038725/149‑1 (MQ=255)
                              aTCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCGATGAGGAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑AAAAAAGATA‑ACGACTATTTTTCTCATTTTGcgttg                                                                                                           >  1:939265/1‑145 (MQ=255)
                               acaaGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTAAAAAAAAAAA‑A‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGc                                                                                                          <  2:1424984/146‑1 (MQ=255)
                                  aGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGTAATATGATTTCCTTTGTTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTCTTTTTCTCATTTTGCAGAGCCat                                                                                                       >  1:1758291/1‑149 (MQ=255)
                                  aGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCAttt                                                                                                                  <  2:61399/138‑1 (MQ=255)
                                  aGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCAat                                                                                                       >  1:900726/1‑149 (MQ=255)
                                  aGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑AAAAAAGATA‑ACGACTATTTTTCTCAttt                                                                                                                  >  1:61399/1‑138 (MQ=255)
                                  aGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑AAAAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCat                                                                                                       >  2:1689120/1‑149 (MQ=255)
                                    acacAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑AAAAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCatat                                                                                                     >  2:201601/1‑149 (MQ=255)
                                         gAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTc                                                                                                <  2:1139827/149‑1 (MQ=255)
                                         gAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTc                                                                                                <  1:1333673/149‑1 (MQ=255)
                                               aagaagTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTcaca                                                                                          >  1:10618/1‑149 (MQ=255)
                                               aagaagTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTcaca                                                                                          <  1:201601/149‑1 (MQ=255)
                                               aagaagTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTcaca                                                                                          >  2:22206/1‑149 (MQ=255)
                                               aagaagTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTGCTCATTTTGCAGTGCCATATTGTTCCTcaca                                                                                          >  2:132615/1‑149 (MQ=255)
                                               aagaagTAGACAACTGACTGATATAAAGAGAGTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCGGGTTTCCAGTCG‑CG‑AAAAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTcaca                                                                                          >  2:1803161/1‑149 (MQ=255)
                                                agaagTAGACAACTGACTGATATCCAGAGATTTCGTCGAGAACTTTCGGTATCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACAt                                                                                         <  1:1526070/149‑1 (MQ=255)
                                                    gTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGcac                                                                                     >  2:161023/1‑149 (MQ=255)
                                                       gACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑AAAAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGcccaca                                                                                  >  2:557252/1‑149 (MQ=255)
                                                       gACAACTGACTGATATCCAGAAATTCGTTTGATCTATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTACCATATTGTTCCTCACAtcccacca                                                                                  >  2:770018/1‑142 (MQ=255)
                                                          aaCTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTg                                                                               >  1:39436/1‑149 (MQ=255)
                                                          aaCTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTg                                                                               <  1:41340/149‑1 (MQ=255)
                                                            ctgactgaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGt                                                                             <  1:1839796/149‑1 (MQ=255)
                                                            ctgactaaTATCCAGAGATTTCTTCCAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGt                                                                             <  1:66143/149‑1 (MQ=255)
                                                             tgactgaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCAAATGCACACATTGGTa                                                                            >  1:500342/1‑149 (MQ=255)
                                                             tgactgaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGGGCTATATTGTTCCTCACCTGCCCACATTGGTa                                                                            >  1:688544/1‑149 (MQ=255)
                                                                 tgaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATATACCA‑AAGGGTGCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGa                                                                        >  1:2040828/1‑149 (MQ=255)
                                                                 tgaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑AAAAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGa                                                                        >  1:1457055/1‑149 (MQ=255)
                                                                   atatCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCCCACATTGGTAATGaaa                                                                      >  2:1603872/1‑149 (MQ=255)
                                                                      tCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGaaaaaa                                                                   >  2:344991/1‑149 (MQ=255)
                                                                       caaaagagTTCTCGGAGAAATATGTTTTCCTTTATTTACCCAGGATGCCGGATTTTCTGGTTAAAAAAAA‑AGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGaaaaaaa                                                                  <  2:1758291/145‑1 (MQ=255)
                                                                        cAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAg                                                                 >  1:1537224/1‑149 (MQ=255)
                                                                         agagATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAACGa                                                                >  1:1145772/1‑149 (MQ=255)
                                                                         agagATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGa                                                                >  1:1782910/1‑149 (MQ=255)
                                                                            gaTTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACaa                                                             <  1:875321/149‑1 (MQ=255)
                                                                              tttCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCATTGCCATATTGTTCCTCACATCTACACATTGCTAATGAAAAAAGAACaaaa                                                           >  2:1368087/1‑149 (MQ=255)
                                                                                 cttcGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAacac                                                        >  1:883874/1‑149 (MQ=255)
                                                                                  ttcGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACg                                                       >  2:2063686/1‑149 (MQ=255)
                                                                                  ttcGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACg                                                       >  1:1167167/1‑149 (MQ=255)
                                                                                  ttcGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑AAAAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAGAGACAAAACACg                                                       >  1:571945/1‑149 (MQ=255)
                                                                                  ttcGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑AAAAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACg                                                       >  2:1673251/1‑149 (MQ=255)
                                                                                   tcGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑AAAAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGa                                                      <  2:559025/149‑1 (MQ=255)
                                                                                        cAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTaa                                                 <  1:1488436/149‑1 (MQ=255)
                                                                                             tGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGt                                                                                                  >  2:859892/1‑95 (MQ=255)
                                                                                             tGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGt                                                                                                  <  1:859892/95‑1 (MQ=255)
                                                                                                tttCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑AAAAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAAt                                         >  1:865899/1‑149 (MQ=255)
                                                                                                  tCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAg                                       <  1:1976177/149‑1 (MQ=255)
                                                                                                  cccTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAg                                       <  1:768149/148‑1 (MQ=255)
                                                                                                    cTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGcc                                     >  1:197575/1‑149 (MQ=255)
                                                                                                     tttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGa                                                                >  1:1250452/1‑121 (MQ=255)
                                                                                                     tttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGa                                                                <  2:1250452/121‑1 (MQ=255)
                                                                                                     tttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCa                                    <  2:1368581/149‑1 (MQ=255)
                                                                                                     tttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑AAAAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACAAGAGGTAAGGCGCAATATCCa                                    >  1:140526/1‑149 (MQ=255)
                                                                                                     tttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGGCG‑CGAA‑AAAAGAAC‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCa                                    <  1:280804/149‑1 (MQ=255)
                                                                                                      ttATTTTCCCAGAAGGCCGGATTTTCCGGTTCCCAGTCGCCA‑T‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATCGTGACTCTCATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAg                                   <  1:710653/149‑1 (MQ=255)
                                                                                                        aTTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑TGAA‑AAAAGATA‑ACGACTATTTTTCTGATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTGATGAAAAAAAGAAAAAACACGAGGCAAGACGCAATACCCAGtt                                 >  2:944710/1‑149 (MQ=255)
                                                                                                        aTTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTca                                                                                            >  2:8511/1‑90 (MQ=255)
                                                                                                        aTTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTca                                                                                            <  1:8511/90‑1 (MQ=255)
                                                                                                         ttttCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGtta                                >  1:2010574/1‑149 (MQ=255)
                                                                                                           ttacctgAAGGCGGGATTTTCGGTTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGttatt                              <  2:670889/143‑1 (MQ=255)
                                                                                                           ttCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGttatt                              >  1:1770924/1‑149 (MQ=255)
                                                                                                            tCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAACCAGttatta                             >  1:949806/1‑149 (MQ=255)
                                                                                                              ccAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAAGACGCGGTAAGGCGCAATAGCCAGTAATTAGa                           >  2:955121/1‑149 (MQ=255)
                                                                                                              ccAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCAATTTGCAGTGCCATATTGTTCCTCACATGCACACAGTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGataataaa                           >  2:1579158/1‑147 (MQ=255)
                                                                                                               cAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGaa                          >  2:1232080/1‑149 (MQ=255)
                                                                                                               cAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGaa                          >  1:1494611/1‑149 (MQ=255)
                                                                                                                   acgCCGGAGTTTCTGGTTTCCAGTAG‑CG‑AAAAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTaa                      <  2:310432/147‑1 (MQ=255)
                                                                                                                   aGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTaa                      <  2:39436/149‑1 (MQ=255)
                                                                                                                      ccGGAATTTCTGGTTTCAAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGGAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGCGGTAAGGCGCATTAGCCAGCTATTAAACTCAAGGa                   >  2:2082106/1‑149 (MQ=255)
                                                                                                                       cgaaTTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGAt                  <  1:1980888/146‑1 (MQ=255)
                                                                                                                       cGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGAt                  >  2:162676/1‑149 (MQ=255)
                                                                                                                          aTTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGaa               <  1:1705044/149‑1 (MQ=255)
                                                                                                                           ttttCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTTTTTTGCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAAt              <  1:389120/149‑1 (MQ=255)
                                                                                                                             ttCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTg            <  1:661041/149‑1 (MQ=255)
                                                                                                                               cTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTggg          <  1:1686021/149‑1 (MQ=255)
                                                                                                                                   tttCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTACAGGTCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGaa      <  1:1437058/149‑1 (MQ=255)
                                                                                                                                      ccAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTg   >  1:324716/1‑149 (MQ=255)
                                                                                                                                       cAGTCG‑CG‑AAAAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGc  >  1:1157101/1‑149 (MQ=255)
                                                                                                                                       cAGTCG‑CG‑AAAAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGc  >  2:1851529/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
TTGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑C‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGC  >  NC_000913_3_pae_tpiA/1543879‑1544161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A