Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A18 F130 I1 R1	38	76.4	2725422	95.3%	2597327	137.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	2,670,017	G→A	G329D (GGC→GAC)	hcaE →	3‑phenylpropionate dioxygenase, large (alpha) subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	2,670,017	0	G	A	98.7%	256.9 / NA	75	G329D (GGC→GAC)	hcaE	3‑phenylpropionate dioxygenase, large (alpha) subunit
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (38/36); minor base C (0/1); total (38/37)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

ATTACCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGGCCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCAGGATGACTCGGAGAACTGG  >  NC_000913_3_pae_tpiA/2669879‑2670156

aTTACCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAg                                                                                                                                  >  2:714372/1‑150 (MQ=255)

   aCCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGTTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTg                                                                                                                               >  4:492818/1‑150 (MQ=255)

     cgcgAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGaa                                                                                                                             <  1:250915/150‑1 (MQ=255)

     cgcgAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGaa                                                                                                                             >  3:470803/1‑150 (MQ=255)

        gAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAgtg                                                                                                                          <  2:400943/150‑1 (MQ=255)

                tGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGtt                                                                                                                  >  4:54152/1‑150 (MQ=255)

                tGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGtt                                                                                                                  <  2:568117/150‑1 (MQ=255)

                 gCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTc                                                                                                                 >  2:268013/1‑150 (MQ=255)

                  ccGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCt                                                                                                                <  3:412023/150‑1 (MQ=255)

                   cGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTg                                                                                                               >  1:725649/1‑150 (MQ=255)

                    gAAGCAGAACAACGTTTAGGTGAAGTTCGCACCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTCCCACGCTCCGCGTCTGGCATCCGCGCGACCCTGATTACGTTGAAGTGTGGGCGTTCTGt                                                                                                              <  2:78614/150‑1 (MQ=255)

                     aaGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTa                                                                                                             <  2:1722/150‑1 (MQ=255)

                       gCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTAtt                                                                                                           <  2:725649/150‑1 (MQ=255)

                        cAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTa                                                                                                          <  2:536656/150‑1 (MQ=255)

                              aacGTTTAGGTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACa                                                                                                    >  4:168968/1‑150 (MQ=255)

                              aacGTTTAGGTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACa                                                                                                    >  4:334175/1‑150 (MQ=255)

                                ggTTTAGGTGAAGTTCGCGCCCTGCGGCTGGCGGGTCATAACAATGTTTTCCCCACGCTTGCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGGGGGCGTTCTGTATTACTGACaaa                                                                                                  <  2:133387/149‑1 (MQ=255)

                                     aGGTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAgccgc                                                                                             <  1:353150/150‑1 (MQ=255)

                                       gTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGAcc                                                                                                                                           <  4:484848/102‑1 (MQ=255)

                                       gTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTgg                                                                                                                       >  2:228797/1‑122 (MQ=255)

                                       gTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTgg                                                                                                                       <  1:228797/122‑1 (MQ=255)

                                        tGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTc                                                                                          <  1:298178/150‑1 (MQ=255)

                                        tGAAGTTCGCGCCCCGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTc                                                                                          <  3:424161/150‑1 (MQ=255)

                                          aaGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCg                                                                                        >  4:513034/1‑150 (MQ=255)

                                             ttCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATg                                                                                     >  3:315952/1‑150 (MQ=255)

                                             ttCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATg                                                                                     >  3:272977/1‑150 (MQ=255)

                                               cgcgCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGaa                                                                                   >  4:55492/1‑150 (MQ=255)

                                                 cgcCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGt                                                                                 <  1:462552/150‑1 (MQ=255)

                                                     cTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTaaa                                                                             <  1:393632/150‑1 (MQ=255)

                                                           cTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCt                                                                       >  1:417501/1‑150 (MQ=255)

                                                             ggCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCttt                                                                     <  1:343189/150‑1 (MQ=255)

                                                                 ggTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGaa                                                                 <  3:200995/150‑1 (MQ=255)

                                                                      tAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAg                                                            >  3:25894/1‑150 (MQ=255)

                                                                       aaCAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGAt                                                                                      <  4:419881/123‑1 (MQ=255)

                                                                       aaCAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGAt                                                                                      >  3:419881/1‑123 (MQ=255)

                                                                            tatTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCAc                                                      <  4:315952/150‑1 (MQ=255)

                                                                              ttttCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTc                                                    >  3:471855/1‑150 (MQ=255)

                                                                              ttttCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTc                                                    <  4:297413/150‑1 (MQ=255)

                                                                                ttCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGt                                                  >  1:827162/1‑150 (MQ=255)

                                                                                    ccACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCtt                                              >  1:343395/1‑150 (MQ=255)

                                                                                    ccACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCtt                                              >  2:710987/1‑150 (MQ=255)

                                                                                     cACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCttt                                             >  2:809073/1‑150 (MQ=255)

                                                                                       cGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTg                                           <  4:286301/150‑1 (MQ=255)

                                                                                       cGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTg                                           <  1:189135/150‑1 (MQ=255)

                                                                                       cGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTg                                           <  3:334175/150‑1 (MQ=255)

                                                                                          tttCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTc                                        >  3:430629/1‑150 (MQ=255)

                                                                                              aTGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGCCCCTGATCAAGGTGAAGTGTGGGCGTCCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTATAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCctgc                                    <  2:684967/150‑1 (MQ=255)

                                                                                                ggCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCctgctg                                  <  3:513034/150‑1 (MQ=255)

                                                                                                 gCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTgg                                 >  1:231011/1‑150 (MQ=255)

                                                                                                  cTAAACGGCACTGCCATACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGt                                >  3:414919/1‑150 (MQ=255)

                                                                                                   tAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGtt                               >  2:700417/1‑150 (MQ=255)

                                                                                                   tAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGtt                               <  4:272977/150‑1 (MQ=255)

                                                                                                   tAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGtt                               >  4:114863/1‑150 (MQ=255)

                                                                                                   tAAACGGCACTGCCACACTCCGCGTCTGCCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGtt                               <  4:470803/150‑1 (MQ=255)

                                                                                                        ggCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGATTTtctc                          >  1:667928/1‑150 (MQ=255)

                                                                                                          cACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGa                        <  4:25894/150‑1 (MQ=255)

                                                                                                             tGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCa                     >  1:54851/1‑150 (MQ=255)

                                                                                                                cacaCTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCAGGa                  <  2:273856/150‑1 (MQ=255)

                                                                                                                cacaCTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGATCTGTATTACTGACAAAACCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCAGGa                  >  4:495940/1‑150 (MQ=255)

                                                                                                                     tCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGaa                                                                                   <  2:175551/80‑1 (MQ=255)

                                                                                                                     tCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGaa                                                                                   >  1:175551/1‑80 (MQ=255)

                                                                                                                        gcgTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGt                                                                                 <  4:111016/79‑1 (MQ=255)

                                                                                                                        gcgTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGt                                                                                 >  3:111016/1‑79 (MQ=255)

                                                                                                                        gcgTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCAGGATGACTCgg          >  3:453947/1‑150 (MQ=255)

                                                                                                                        gcgTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCAGGATGACTCgg          >  3:453977/1‑150 (MQ=255)

                                                                                                                         cgTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCAGGATGACTCGga         <  3:54152/150‑1 (MQ=255)

                                                                                                                         cgTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCAGGATGACTCGga         <  3:492818/150‑1 (MQ=255)

                                                                                                                             tGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCAGGa                  >  2:818543/1‑137 (MQ=255)

                                                                                                                             tGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCAGGa                  <  1:818543/137‑1 (MQ=255)

                                                                                                                              ggCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCAGGATGACTCGGAGAACt    >  3:469695/1‑150 (MQ=255)

                                                                                                                              ggCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCAGGATGACTCGGAGAACt    >  1:809327/1‑150 (MQ=255)

                                                                                                                               gCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCAGGATGACTCGGAGAACTg   <  4:39384/150‑1 (MQ=255)

                                                                                                                                cATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCAGGATGACTCGGAGAACTgg  >  2:411587/1‑150 (MQ=255)

                                                                                                                                  tCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAgccgcc                                                                                            >  1:746550/1‑58 (MQ=255)

                                                                                                                                  tCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAgccgcc                                                                                            <  2:746550/58‑1 (MQ=255)

ATTACCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGGCCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCAGGATGACTCGGAGAACTGG  >  NC_000913_3_pae_tpiA/2669879‑2670156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A