Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A16 F141 I1 R1	26	83.5	3170358	94.1%	2983306	130.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	1,544,022	1 bp→AA	coding (39/1389 nt)	narU ←	nitrate/nitrite transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	1,544,021	1	.	A	84.3%	278.3 / 46.9	89	coding (40/1389 nt)	narU	nitrate/nitrite transporter
Reads supporting (aligned to +/- strand): ref base . (10/4); new base A (44/31); total (54/35)
Fisher's exact test for biased strand distribution p-value = 5.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	NC_000913_3_pae_tpiA	1,544,022	0	C	A	98.9%	318.3 / NA	89	L13F (TTG→TTT)	narU	nitrate/nitrite transporter
Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (54/34); total (54/35)
Fisher's exact test for biased strand distribution p-value = 3.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑C‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGATTGAAAG  >  NC_000913_3_pae_tpiA/1543880‑1544171

|||

tGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑A‑aaa‑aaga                                                                                                                                                 >  2:521553/1‑147 (MQ=255)

tGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑A‑aaa‑aaga                                                                                                                                                 >  3:264563/1‑147 (MQ=255)

  agaTTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑A‑aaa‑aagata                                                                                                                                               <  3:98418/150‑6 (MQ=255)

    aTTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAAc                                                                                                                                             >  1:510984/1‑150 (MQ=255)

      tAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGa                                                                                                                                           >  2:643751/1‑150 (MQ=255)

           gTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTAtt                                                                                                                                      <  3:184795/150‑1 (MQ=255)

              aCTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTAttttt                                                                                                                                   <  1:60117/150‑1 (MQ=255)

              aCTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTAttttt                                                                                                                                   <  1:758526/150‑1 (MQ=255)

                     tAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCAttt                                                                                                                            >  4:316112/1‑150 (MQ=255)

                         tAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCa                                                                                                                        >  3:21629/1‑150 (MQ=255)

                           gCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGt                                                                                                                      >  1:87014/1‑150 (MQ=255)

                            cATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTg                                                                                                                     <  1:279479/150‑1 (MQ=255)

                               ccAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCa                                                                                                                  >  3:425937/1‑150 (MQ=255)

                                 aGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCata                                                                                                                >  1:968353/1‑150 (MQ=255)

                                     acaGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCata                                                                                                                <  3:323848/146‑1 (MQ=255)

                                     acaGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCata                                                                                                                >  4:323848/1‑146 (MQ=255)

                                     acaGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGt                                                                                                            >  3:410274/1‑150 (MQ=255)

                                       aGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTc                                                                                                          <  1:643751/150‑1 (MQ=255)

                                       aGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTc                                                                                                          >  3:541170/1‑150 (MQ=255)

                                           ggCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTAAAAAAAAAAAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTca                                                                                                      <  2:229515/150‑1 (MQ=255)

                                              aagaagTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACAt                                                                                                   >  1:21568/1‑150 (MQ=255)

                                              aagaagTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑AAAAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACAt                                                                                                   >  1:487258/1‑150 (MQ=255)

                                                   gTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCa                                                                                                                        <  3:464250/124‑1 (MQ=255)

                                                   gTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑AAAAA‑A‑GATAACGACTATTTTTCTCATTTTGCa                                                                                                                        >  4:464250/1‑124 (MQ=255)

                                                     aGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCGGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGcacaca                                                                                            >  3:109095/1‑150 (MQ=255)

                                                      gACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTTTCTTTTTGCAGTGCCATATTGTTCCTCACATGCACACAt                                                                                           >  4:317366/1‑150 (MQ=255)

                                                         aaCTGACTGATATCCGGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTgg                                                                                        <  4:264563/150‑1 (MQ=255)

                                                         aaCTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATGACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTgg                                                                                        >  4:281649/1‑150 (MQ=255)

                                                         aaCTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTgg                                                                                        >  2:47449/1‑150 (MQ=255)

                                                           ctgactgaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTa                                                                                      >  3:495809/1‑150 (MQ=255)

                                                            tgactgaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTcaca                                                                                                    <  4:291892/135‑1 (MQ=255)

                                                            tgactgaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTcaca                                                                                                    >  3:291892/1‑135 (MQ=255)

                                                              actgaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATg                                                                                   <  1:935132/150‑1 (MQ=255)

                                                                tgaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGaa                                                                                 >  2:805726/1‑150 (MQ=255)

                                                                tgaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGaa                                                                                 >  3:576852/1‑150 (MQ=255)

                                                                  atatCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTCTTTTCCCAGAAGGCAGGATTTTCTGGTTTCCAGTCGCG‑A‑AAACA‑GATAACGACTATTTTTCTCATTTTGGAGTGCCATATTGTTCCTCACATGCACACATTTGTAATGaaaa                                                                               <  4:498536/150‑1 (MQ=255)

                                                                  atatCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑AAAAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGaaaa                                                                               >  4:419155/1‑150 (MQ=255)

                                                                     tacAGAGATTGCTTCGAGAAATATGTTTTCGTGTATTTTCCCAGAAGGCAGGAGTTTCTGGTTTCCAGTGGCGAC‑AAA‑A‑GATAACGACTATGTTTCTCATTTTGCAGTGCTATATTGTTCCCCCCATGCACACATTGGTAATGaaaaaaa                                                                            <  2:957319/148‑1 (MQ=255)

                                                                     tCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGaaaaaaa                                                                            >  3:442748/1‑150 (MQ=255)

                                                                      ccAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTGGCG‑A‑AAA‑ACGATAACGACTATTTTTCTTATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAg                                                                           >  2:598621/1‑150 (MQ=255)

                                                                      ccAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAg                                                                           >  3:140381/1‑150 (MQ=255)

                                                                      ccAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAg                                                                           >  4:402491/1‑150 (MQ=255)

                                                                      ccAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAg                                                                           >  2:262810/1‑150 (MQ=255)

                                                                      ccAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑AAAAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAg                                                                           >  3:344822/1‑150 (MQ=255)

                                                                       cAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGcc                                                                                                                   >  1:592643/1‑109 (MQ=255)

                                                                       cAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGcc                                                                                                                   <  2:592643/109‑1 (MQ=255)

                                                                       cAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGa                                                                          <  3:461251/150‑1 (MQ=255)

                                                                        agagCTTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑AAAAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGAc                                                                         >  2:490624/1‑150 (MQ=255)

                                                                        agagATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGAc                                                                         >  4:96468/1‑150 (MQ=255)

                                                                        agagATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGAc                                                                         <  2:510984/150‑1 (MQ=255)

                                                                         gagaTTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACa                                                                        >  3:378082/1‑150 (MQ=255)

                                                                               tcttcGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCGGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAacac                                                                  >  1:1079/1‑150 (MQ=255)

                                                                               tcttcGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑AAAAA‑A‑GATAAAGACTATTTTTCTCATTTTGCAGTGAAATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAacac                                                                  <  1:634452/150‑1 (MQ=255)

                                                                                 ttcGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGa                                                                >  3:196665/1‑150 (MQ=255)

                                                                                     aGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGc                                                                                                                    >  1:791580/1‑94 (MQ=255)

                                                                                     aGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGc                                                                                                                    <  2:791580/94‑1 (MQ=255)

                                                                                     aGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTAGTAATGAAAAAAAGACAAAACACGAGGTa                                                            <  4:21629/150‑1 (MQ=255)

                                                                                         atatGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑AAAAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGgc                                                        >  2:266097/1‑150 (MQ=255)

                                                                                            tGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTc                                                                                                          <  2:275019/97‑1 (MQ=255)

                                                                                            tGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑AAAAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTc                                                                                                          >  1:275019/1‑97 (MQ=255)

                                                                                                 tCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGc                                                >  4:172185/1‑150 (MQ=255)

                                                                                                   cTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCa                                              <  1:812014/150‑1 (MQ=255)

                                                                                                    tttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCat                                                                                                                 <  2:738056/82‑1 (MQ=255)

                                                                                                    tttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCat                                                                                                                 >  1:738056/1‑82 (MQ=255)

                                                                                                    tttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCa                                              >  3:220976/1‑149 (MQ=255)

                                                                                                    tttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCa                                              <  4:220976/149‑1 (MQ=255)

                                                                                                       aTTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGtta                                          <  2:879889/150‑1 (MQ=255)

                                                                                                         tttCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGAc                                                                         <  1:32290/117‑1 (MQ=255)

                                                                                                         tttCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGAc                                                                         >  2:32290/1‑117 (MQ=255)

                                                                                                            cccAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGa                                     >  4:544231/1‑150 (MQ=255)

                                                                                                            cccAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGa                                     <  3:172185/150‑1 (MQ=255)

                                                                                                             ccAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGaa                                    <  2:751057/150‑1 (MQ=255)

                                                                                                              cAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAAt                                   >  2:957176/1‑150 (MQ=255)

                                                                                                              cAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAAt                                   >  1:831189/1‑150 (MQ=255)

                                                                                                              cAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAAt                                   >  2:947300/1‑150 (MQ=255)

                                                                                                                gAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑AAAAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTa                                 <  4:541170/150‑1 (MQ=255)

                                                                                                                 aaGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTaa                                >  4:189152/1‑150 (MQ=255)

                                                                                                                  aGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAg                               <  2:87014/150‑1 (MQ=255)

                                                                                                                      cGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCa                                              <  4:513623/131‑1 (MQ=255)

                                                                                                                      cGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCa                                              >  3:513623/1‑131 (MQ=255)

                                                                                                                      cGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCa                                              <  1:578098/131‑1 (MQ=255)

                                                                                                                      cGGATTTTCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCa                                              >  2:578098/1‑131 (MQ=255)

                                                                                                                          ttttCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTACCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATGAAGAAGGAAta                       >  4:341706/1‑149 (MQ=255)

                                                                                                                             tCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTggg                    <  2:463008/150‑1 (MQ=255)

                                                                                                                             tCTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTggg                    >  3:352152/1‑150 (MQ=255)

                                                                                                                              cTGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGt                   <  4:495809/150‑1 (MQ=255)

                                                                                                                               tGGTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTg                  >  3:599030/1‑150 (MQ=255)

                                                                                                                                 gTTTCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGaa                >  1:659487/1‑150 (MQ=255)

                                                                                                                                   ttCCAGTCGCGAA‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGt              <  4:140381/150‑1 (MQ=255)

                                                                                                                                            gcgaaaaa‑a‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGATTg      >  3:39222/6‑150 (MQ=255)

                                                                                                                                              aaaaaa‑a‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGGATTAAGGATGAATTGGGTGAAGTGCTGATTGaa    <  3:558577/147‑1 (MQ=255)

                                                                                                                                               aaaaa‑a‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGATTGaaa   <  1:947300/148‑1 (MQ=255)

                                                                                                                                                aaaa‑a‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGATTGAAAg  <  3:402491/149‑1 (MQ=255)

|||

TGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑C‑AAA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGATTGAAAG  >  NC_000913_3_pae_tpiA/1543880‑1544171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A