Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A22 F64 I1 R1 18 58.3 2092080 92.1% 1926805 145.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 1,544,022 1 bp→AA coding (39/1389 nt) narU ← nitrate/nitrite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA1,544,0211.A82.3% 159.1 / 30.7 62coding (40/1389 nt)narUnitrate/nitrite transporter
Reads supporting (aligned to +/- strand):  ref base . (8/3);  new base A (31/20);  total (39/23)
Fisher's exact test for biased strand distribution p-value = 5.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_000913_3_pae_tpiA1,544,0220CA96.8% 176.0 / NA 62L13F (TTG→TTT) narUnitrate/nitrite transporter
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (38/22);  total (39/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑G‑C‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGATTGA  >  NC_000913_3_pae_tpiA/1543879‑1544168
                                                                                                                                                  |||                                                                                                                                                  
ttGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCAAGTCT‑C‑G‑C‑a‑aaaa                                                                                                                                               >  1:768205/1‑148 (MQ=255)
   agaTTAACGGTAACTGCGCTAAATAGCATCCAGGCACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑G‑A‑a‑aaaagat                                                                                                                                            >  2:543823/1‑145 (MQ=255)
   agaTTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑G‑A‑a‑aaaagat                                                                                                                                            >  2:564796/1‑145 (MQ=255)
           ggTAACTGCGCTAAATAGACTACAGACACAGAAGGCAACAACTCTGGTGCTGAACCATCTCAACTGAAGTTGTCTAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTATGGTTTCCAGTCG‑CAA‑A‑A‑AAAGATAACGACTa                                                                                                                                     >  2:220363/1‑149 (MQ=255)
                cTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑A‑AAAGATAACGACTAttttt                                                                                                                                <  2:387942/149‑1 (MQ=255)
                  gcgcTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑A‑AAAGATAACGACTAtttt                                                                                                                                 <  2:14959/146‑1 (MQ=255)
                  gcgcTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑A‑AAAGATAACGACTAtttt                                                                                                                                 >  1:14959/1‑146 (MQ=255)
                    gcTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCATTTATTTTACAAGAAGGCCGGATTTTCTGGTTTCGAGTCA‑C‑GAA‑A‑AAAGATAAAGACTAtttttacta                                                                                                                            >  1:397820/1‑145 (MQ=255)
                          tAGCATCCAGACACAGAAGGCAAGAAGGAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGc                                                                                                                      >  2:552499/1‑149 (MQ=255)
                           aGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCa                                                                                                                     <  1:663775/149‑1 (MQ=255)
                                ccAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCT‑C‑GAA‑A‑AAAGATAACGACTATTTTTCTAATTTTGCAGGGcc                                                                                                                >  2:70446/1‑149 (MQ=255)
                                      acaGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGGGCCATATTg                                                                                                          >  2:829908/1‑149 (MQ=255)
                                        aGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGtt                                                                                                        >  2:738779/1‑149 (MQ=255)
                                        aGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTATGGTTTCCAGTCG‑C‑G‑AAA‑AAAGATAACGACTATTTTTCTTATTTTGCAGTTCCATATTGtt                                                                                                        >  2:1011560/1‑149 (MQ=255)
                                         gAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTc                                                                                                       <  1:543823/149‑1 (MQ=255)
                                         gAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAACGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑A‑AAAGATAACGTCTATTTTTCTCATTTTGCAGTGCCATATTGTTc                                                                                                       <  2:990336/149‑1 (MQ=255)
                                         caaGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCACTCG‑A‑GAC‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTc                                                                                                       <  2:527416/148‑1 (MQ=255)
                                             gCAAGGAGTAGACAACTGACCTATATCCAGAGACTTCTGCGAGCAATATGTTTTCCTTTATTTGCCCAGAAGGCCGGATTTGCTGTTTAACAGTAG‑C‑GAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTca                                                                                                   <  2:56790/149‑1 (MQ=255)
                                             gCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑A‑AAAGATCACGACTATTTTTGCCAGCTTGCAGTGCCATATTGTTCCTca                                                                                                   >  1:661146/1‑149 (MQ=255)
                                              cAAGAAGTAGACCACTGACTGATATCCAGAGATTTCTACGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑A‑AAAGATAACGACTATTTTTCTAATTTTCCAGTGCCATATTGTTCCTcac                                                                                                  >  2:616478/1‑149 (MQ=255)
                                               aagaagTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGGTTTTCTGGTTTCCAGTCG‑C‑GAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTACCATATTGTTCCTaaca                                                                                                 >  2:897871/1‑149 (MQ=255)
                                                 gaagTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCGGGATTTTCTGGTTTCCCGTCG‑C‑GAA‑A‑AAAGATAACGACTATTTTTATCATTTTGCAGTGCCATATTGTTCCTCACATg                                                                                               >  2:143760/1‑149 (MQ=255)
                                                   agaaGACAACTAACTGATATCCAGAGATTCCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGca                                                                                             <  1:865086/146‑1 (MQ=255)
                                                   aataaaCAACTAACTGATATCCAAAAATTTCTTCAAGCAAAAACATACACGTTATTTTCCCAGAAGGCCGGATTTTCTGGTTGCCAGTCG‑C‑GAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGca                                                                                             <  1:338250/144‑1 (MQ=255)
                                                     tAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGcaca                                                                                           >  1:222555/1‑149 (MQ=255)
                                                        aCAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACAt                                                                                        >  2:7844/1‑149 (MQ=255)
                                                            ctgactgaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGt                                                                                    <  1:738779/149‑1 (MQ=255)
                                                            ctgactgaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGt                                                                                    >  1:280109/1‑149 (MQ=255)
                                                                 tgaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGa                                                                               >  1:292459/1‑149 (MQ=255)
                                                                   atatCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCGAGAGTTTCTGGTTTCCAGTCG‑C‑GAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTTGTAATGaaa                                                                             >  1:464522/1‑149 (MQ=255)
                                                                   atatCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTGAGGaaa                                                                             >  2:397639/1‑149 (MQ=255)
                                                                       ccAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACCCATTGGTAATGaaaaaaa                                                                         >  2:757084/1‑149 (MQ=255)
                                                                       caagagATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGTTTTTCTGGTTTCCAGTCG‑C‑GAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGaaaaaaa                                                                         <  1:994268/147‑1 (MQ=255)
                                                                        cAGAGATTTCTTCGAGCAATATGTTTTCCTTTCTTTTCACAGCAATCCCGATTTTCTGGTTTCCAGTAG‑C‑GAA‑A‑AAAGATAACGACTATTTTTCTCCTTTTGCAGTGCCATATTGTTCCTCACATTCACACATTGTTAATGAAAAAAAg                                                                        >  2:174279/1‑149 (MQ=255)
                                                                         agagATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑A‑AAAGATAACGTCTATTTTTCTCATTTTTCGGTGCCATATTGTTTCTAACATGCACAAATTGCTATTGAAAATAAGa                                                                       >  1:10544/1‑149 (MQ=255)
                                                                          gagaTTTCTGCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGAc                                                                      >  2:10683/1‑149 (MQ=255)
                                                                                ttttcGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATGTTCTGGTTTCCAGTCG‑C‑GAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAaca                                                                <  1:788984/147‑1 (MQ=255)
                                                                                tcttcGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATAGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACaaaaaa                                                                >  1:648104/1‑147 (MQ=255)
                                                                                 cttcGAGCAATTTGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTTGTTTCCAGTCG‑C‑GAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAacac                                                               <  1:1004694/149‑1 (MQ=255)
                                                                                  ttcGAGGAATAGGTTTTCCTTTATGTACCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑G‑AAA‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACg                                                              <  2:806674/149‑1 (MQ=255)
                                                                                  ttcGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTTG‑C‑GAA‑A‑AAAGATAACGACTATTTTTTTTTGGTTGCAGTGTCATATTGTTTGTCACATGTACAGTTTGGTAATGAAAATATAACAAAACACg                                                              >  1:502199/1‑149 (MQ=255)
                                                                                  ttcGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGCGG‑C‑GAA‑A‑AAAGATAACGACTCTTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAAACATTGGTAATGAAAAAAAGACAAAACACg                                                              >  1:379336/1‑149 (MQ=255)
                                                                                   tggAGCAATATTGTTTCCTTTATTGTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑G‑A‑ATAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCATCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGa                                                             <  2:927300/147‑1 (MQ=255)
                                                                                       gCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑G‑AAA‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCAAATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTa                                                         >  1:741260/1‑149 (MQ=255)
                                                                                         aaTATTTTTTCCTTTATTTGCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAg                                                       <  1:650959/149‑1 (MQ=255)
                                                                                            atGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGgcg                                                    <  1:10683/149‑1 (MQ=255)
                                                                                               ttttCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCaa                                                 >  2:62756/1‑149 (MQ=255)
                                                                                               ttttCCTTGATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCaa                                                 <  2:940655/149‑1 (MQ=255)
                                                                                               tttaCCTTTATTTTCCCAGAATTCCGGATTTTTTGGTTTCCAATCGTT‑A‑A‑A‑AAAGATAATGACTTTTTTTTTCTTTTTGCAGTGCCACATTGTCCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCaa                                                 <  1:295882/149‑1 (MQ=255)
                                                                                                  tCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGATAATGAACAAAAGACAAAACAAGGGATAAGGCGCAagag                                              >  2:282707/1‑149 (MQ=255)
                                                                                                     tttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTACTGAAAAAAAGACAAAACACGAGGCAAGGCGCAATAGCCa                                           >  2:543496/1‑149 (MQ=255)
                                                                                                           ttCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGttatt                                     <  1:62756/149‑1 (MQ=255)
                                                                                                              ccAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGa                                  >  1:627793/1‑149 (MQ=255)
                                                                                                              ccAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑G‑AAA‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGAAAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGa                                  >  1:627852/1‑149 (MQ=255)
                                                                                                                   aGGCCGGATTTTCTGGTACCCAGTCG‑C‑GAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTaa                             <  2:685851/149‑1 (MQ=255)
                                                                                                                       cGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑A‑AAAGATAAGAACTATTTTTCTCATTTTACAGTGCCATATTGTTCATCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGGTGGAAGGCGCAATAGCAAGTTACTAAAATTAAGGaa                         >  2:687505/1‑148 (MQ=255)
                                                                                                                       cGGATTTTCTGGTTTCCAGTCG‑C‑GAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGCCAAAACACGAGGTAAGACGCAAAAGCCAGTTATTAGAATTAAGAAt                         >  2:451201/1‑149 (MQ=255)
                                                                                                                        ggATTTTCTGGTTTCCAGTCG‑C‑GAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAAACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATg                        <  1:564796/149‑1 (MQ=255)
                                                                                                                        ggATTTTCTGGTTTCCAGTAG‑C‑T‑AAA‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAACACACGCGGTAAGGCGCAAAAGCCAGTTATTAGAATTAAGGAtc                        >  2:739334/1‑148 (MQ=255)
                                                                                                                                 ggTTTCCAGTCG‑C‑GAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCCGTGCCATATTGTTCCTCACACGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAGTAGCCAGTCATTAGGATTAAGGATGAATTGGGTg               >  1:944179/1‑149 (MQ=255)
                                                                                                                                    ttCCAGTCG‑C‑GAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAg            <  1:991975/149‑1 (MQ=255)
                                                                                                                                     tCCAGTCG‑C‑GAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGt           >  2:842909/1‑149 (MQ=255)
                                                                                                                                             tcgcgaaa‑aaaGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGGGCTGa      >  1:492264/8‑149 (MQ=255)
                                                                                                                                                 gaaa‑aaaGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGATTGa  <  2:292459/146‑1 (MQ=255)
                                                                                                                                                  |||                                                                                                                                                  
TTGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑C‑G‑C‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGATTGA  >  NC_000913_3_pae_tpiA/1543879‑1544168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A