Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F1 I2 R1 67 32.5 1435938 95.9% 1377064 112.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,688,142 G→A A79V (GCC→GTC)  fumA ← fumarate hydratase (fumarase A), aerobic Class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,688,1420GA88.2% 39.7 / ‑1.5 17A79V (GCC→GTC) fumAfumarate hydratase (fumarase A), aerobic Class I
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (8/7);  total (9/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

TACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAATCTCCTGCCCT  >  NC_000913/1688015‑1688273
                                                                                                                               |                                                                                                                                   
tACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCa                                                                                                                          >  2:515282/1‑139 (MQ=255)
    cGCTGCCCTTTTTTACCAACAATTATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGACCTCCGGGTCACGCa                                                                                                                      <  1:700302/139‑1 (MQ=255)
        gCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGACCTCCGGGTCACGCAGAAt                                                                                                                  >  1:183692/1‑139 (MQ=255)
            ttttttACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGACCTCCGGGTCACGCAGAATGTCg                                                                                                              <  2:183692/139‑1 (MQ=255)
              ttttACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGACCTCCGGGTCACGCAGAATGTCGGc                                                                                                            <  2:633276/139‑1 (MQ=255)
                                          aTCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACgcagc                                                                                <  1:515282/139‑1 (MQ=255)
                                                    gTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGACCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAacgacg                                                                      >  1:19874/1‑139 (MQ=255)
                                                                              gTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGACCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAAc                                            <  1:311536/139‑1 (MQ=255)
                                                                               tCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGACCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACa                                           >  2:644049/1‑139 (MQ=255)
                                                                                       aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGACCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGtt                                      >  2:130379/1‑136 (MQ=255)
                                                                                       aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGACCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGtt                                      <  1:130379/136‑1 (MQ=255)
                                                                                                 tGCAGCGCCACATATTTATCATTTTCGCTGACCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTg                         <  1:644049/139‑1 (MQ=255)
                                                                                                 tGCAGCGCCACATATTTATCATTTTCGCTGACCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTg                         <  2:19874/139‑1 (MQ=255)
                                                                                                                   tCATTTTCGCTGACCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAATCTCCt       >  1:78757/1‑139 (MQ=255)
                                                                                                                     aTTTTCGCTGACCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAATCTCCTGc     >  1:398164/1‑139 (MQ=255)
                                                                                                                       tttCGCTGACCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAATCTCCTGccc   >  1:199846/1‑139 (MQ=255)
                                                                                                                        ttCGCTGACCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAATCTCCTGCCCt  >  1:456055/1‑139 (MQ=255)
                                                                                                                               |                                                                                                                                   
TACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAATCTCCTGCCCT  >  NC_000913/1688015‑1688273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A