Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F1 I1 R1
|
63 |
64.8 |
2934156 |
93.7% |
2749304 |
111.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,691,529 |
(A)6→7 |
intergenic (+169/+57) |
ydgA → / ← uidC |
DUF945 family protein/putative outer membrane porin for beta‑glucuronides porin protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,691,523 | 1 | . | A | 100.0%
| 152.0
/ NA
| 45 | intergenic (+163/+63) | ydgA/uidC | DUF945 family protein/putative outer membrane porin for beta‑glucuronides porin protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (22/23); total (22/23) |
CACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAT‑AAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATACTCATTTTCACATCCCAGTAATTCAGGCGATCGCCCAGCGTA > NC_000913/1691388‑1691655
|
cACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAT‑aaa < 1:710047/139‑1 (MQ=255)
ccGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAT‑aaaaaaa > 1:495971/1‑138 (MQ=255)
cGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCTGCAACACGTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAT‑aaaaaaac > 2:1050192/1‑137 (MQ=255)
ggCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAT‑aaaaaaacg > 2:702741/1‑136 (MQ=255)
gAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTcaa < 2:950650/139‑1 (MQ=255)
aTTGCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCg > 1:938833/1‑139 (MQ=255)
tGCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGga > 2:1114638/1‑139 (MQ=255)
gCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGgag > 1:802375/1‑139 (MQ=255)
cTACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTtctct > 1:1195958/1‑139 (MQ=255)
tACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCCCTTTGTCAACAATCTGAAACGCCGGAGATTTTtctctc < 1:123049/139‑1 (MQ=255)
tACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTtctctc > 1:786372/1‑139 (MQ=255)
tACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTtctctc < 2:647067/139‑1 (MQ=255)
ttATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCg < 1:1050192/139‑1 (MQ=255)
aTCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCg > 2:1113790/1‑137 (MQ=255)
aTCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCg < 1:1113790/137‑1 (MQ=255)
tCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTa > 1:30776/1‑139 (MQ=255)
aGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTAtt > 1:190265/1‑139 (MQ=255)
gCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAT‑aaaaaaacgcg > 2:662772/1‑82 (MQ=255)
gCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAT‑aaaaaaacgcg < 1:662772/87‑6 (MQ=255)
gCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAT‑aaaaa > 2:73473/1‑81 (MQ=255)
gCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAT‑aaaaa < 1:73473/81‑1 (MQ=255)
ccTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACt < 1:209561/139‑1 (MQ=255)
ccTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACt > 2:278990/1‑139 (MQ=255)
cTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACtt < 2:1346252/139‑1 (MQ=255)
ccTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCa < 1:314565/139‑1 (MQ=255)
cacTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTc < 1:702741/139‑1 (MQ=255)
tttGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATa < 1:758605/139‑1 (MQ=255)
tttGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATa < 2:1299322/139‑1 (MQ=255)
tGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACt > 2:173456/1‑139 (MQ=255)
aTTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGaa < 1:795797/139‑1 (MQ=255)
aTGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATACt < 2:30776/139‑1 (MQ=255)
tGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATACTc > 2:999910/1‑139 (MQ=255)
ttGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATACTCATTTTca < 1:173456/139‑1 (MQ=255)
tAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATACTCATTTTCACAt < 2:802375/139‑1 (MQ=255)
tGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATACTCATTTTCACATCCCAg > 2:720785/1‑139 (MQ=255)
gATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATACTCATTTTca > 1:817630/1‑129 (MQ=255)
gATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATACTCATTTTca < 2:817630/129‑1 (MQ=255)
gATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATACTCATTTTCACATCCCAGTa > 1:1103632/1‑139 (MQ=255)
ggCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCg > 2:1416410/1‑79 (MQ=255)
ggCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCg < 1:1416410/79‑1 (MQ=255)
gTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTtctctc > 2:644934/1‑71 (MQ=255)
gTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTtctctc < 1:644934/71‑1 (MQ=255)
ttACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCgg < 2:247943/56‑1 (MQ=18)
ttACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCgg > 1:247943/1‑56 (MQ=18)
ttACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCg < 2:340085/55‑1 (MQ=18)
ttACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCg > 1:340085/1‑55 (MQ=18)
ttACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATACTCATTTTCACATCCCAGTAATTCAGGCg > 2:354387/1‑138 (MQ=255)
ttACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATACTCATTTTCACATCCCAGTAATTCAGGCg < 1:354387/138‑1 (MQ=255)
ccgcATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATa < 2:593450/97‑1 (MQ=255)
ccgcATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATa > 1:593450/1‑97 (MQ=255)
cgcATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATACTCATTTTCACATCCCAGTAATTCAGGCGATCGccc > 2:1075904/1‑139 (MQ=255)
cgcATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATACTCATTTTCACATCCCAGTAATTCAGGCGATCGccc > 1:77071/1‑139 (MQ=255)
cGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATACTCATTTTCACATCCCAGTAATTCAGGCGATCGCCCAGCGTa < 2:190265/139‑1 (MQ=255)
|
CACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAT‑AAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATACTCATTTTCACATCCCAGTAATTCAGGCGATCGCCCAGCGTA > NC_000913/1691388‑1691655
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
