Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F1 I2 R1
|
92 |
50.6 |
2362856 |
94.3% |
2228173 |
111.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,585,424 |
A→G |
Y355H (TAT→CAT) |
ydeP ← |
putative oxidoreductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,585,424 | 0 | A | G | 100.0%
| 110.8
/ NA
| 30 | Y355H (TAT→CAT) | ydeP | putative oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (17/13); total (17/13) |
GATACCCGCACCAGGCTTGCCAATGTTACCTTTCATCAACAGCAGATTGACCAGTTGCTGTACGTTCTGGGTACCATGTTCGTGCTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATATGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATC > NC_000913/1585290‑1585556
|
gATACCCGCACCAGGCTTGCCAATGTTACCTTTCATCAACAGCAGATTGACCAGTTGCTGTACGTTCTGGGTACCATGTTCGTGCTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCg < 1:904040/139‑1 (MQ=255)
cccGCACCAGGCTTGCCAATGTTACCTTTCATCAACAGCAGATTGACCAGTTGCTGTACGTTCTGGGTACCATGTTCGTGCTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAg > 2:708552/1‑139 (MQ=255)
ccGCACCAGGCTTGCCAATGTTACCTTTCATCAACAGCAGATTGACCAGTTGCTGTACGTTCTGGGTACCATGTTCGTGCTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGc < 1:636350/139‑1 (MQ=255)
cAGGCTTGCCAATGTTACCTTTCATCAACAGCAGATTGACCAGTTGCTGTACGTTCTGGGTACCATGTTCGTGCTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTc > 2:590157/1‑139 (MQ=255)
ggCTTGCCAATGTTACCTTTCATCAACAGCAGATTGACCAGTTGCTGTAAGTTCTGGGTACCATGTTCGTGCTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGACATGTGCGTCAGCCAGTTCgg < 1:708552/139‑1 (MQ=255)
gCCAATGTTACCTTTCATCAACAGCAGATTGACCAGTTGCTGTACGTTCTGGGTACCATGTTCGTGCTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGAt < 1:203846/138‑1 (MQ=255)
gCCAATGTTACCTTTCATCAACAGCAGATTGACCAGTTGCTGTACGTTCTGGGTACCATGTTCGTGCTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGAt > 2:203846/1‑138 (MQ=255)
cTTTCATCAACAGCAGATTGACCAGTTGCTGTACGTTCTGGGTACCATGTTCGTGCTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGATTTGTGTCTGACt < 1:590157/139‑1 (MQ=255)
ttGACCAGTTGCTGTACGTTCTGGGTACCATGTTCGTGCTGAGTGATCCCCATTCCGTAACAGATAATGGAGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGtt < 2:127082/139‑1 (MQ=255)
tGACCAGTTGCTGTACGTTCTGGGTACCATGTTCGTGCTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGATTTGTGTCTGACt < 1:1091462/121‑1 (MQ=255)
tGACCAGTTGCTGTACGTTCTGGGTACCATGTTCGTGCTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGATTTGTGTCTGACt > 2:1091462/1‑121 (MQ=255)
cATGTTCGTGCTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGtt > 2:765285/1‑111 (MQ=255)
cATGTTCGTGCTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGtt < 1:765285/111‑1 (MQ=255)
cATGTTCGTGCTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAAc > 2:801779/1‑139 (MQ=255)
gTGCTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACgg > 1:673566/1‑139 (MQ=255)
aGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGAt > 2:1179308/1‑69 (MQ=255)
aGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGAt < 1:1179308/69‑1 (MQ=255)
aGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGATTTGTGTCTGACt < 1:494167/81‑1 (MQ=255)
aGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGATTTGTGTCTGACt > 2:494167/1‑81 (MQ=255)
tAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGAttt > 2:469614/1‑55 (MQ=255)
tAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGAttt < 1:469614/55‑1 (MQ=255)
tAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGa < 1:596032/85‑1 (MQ=255)
tAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGa > 2:596032/1‑85 (MQ=255)
tAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACgg > 1:548255/1‑139 (MQ=255)
aTAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGATTTGGGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCg > 2:312867/1‑139 (MQ=255)
tGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTg > 1:1143681/1‑139 (MQ=255)
tGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGATTTgtgt < 2:1029510/46‑1 (MQ=255)
tGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGATTTgtgt > 1:1029510/1‑46 (MQ=255)
cGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATaa > 2:297794/1‑139 (MQ=255)
cGGCATGTGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGtcatc > 2:703837/1‑139 (MQ=255)
|
GATACCCGCACCAGGCTTGCCAATGTTACCTTTCATCAACAGCAGATTGACCAGTTGCTGTACGTTCTGGGTACCATGTTCGTGCTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATATGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATC > NC_000913/1585290‑1585556
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
