Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I2 R1	92	50.6	2362856	94.3%	2228173	111.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,751,030	G→A	intergenic (‑17/+47)	ydhX ← / ← ydhW	putative 4Fe‑4S ferridoxin‑type protein, FNR, Nar, NarP‑regulated protein, putative subunit of YdhYVWXUT oxidoreductase complex/FNR, Nar, NarP‑regulated protein, putative subunit of YdhYVWXUT oxidoreductase complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,751,030	0	G	A	100.0%	175.9 / NA	50	intergenic (‑17/+47)	ydhX/ydhW	putative 4Fe‑4S ferridoxin‑type protein, FNR, Nar, NarP‑regulated protein, putative subunit of YdhYVWXUT oxidoreductase complex/FNR, Nar, NarP‑regulated protein, putative subunit of YdhYVWXUT oxidoreductase complex
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (26/24); total (26/24)

TGGCATACCGAACTTCCTTTTCTTGCCTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTAC  >  NC_000913/1750899‑1751164

tGGCATACCGAACTTCCTTTTCTTGCCTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATACAGTGGc                                                                                                                                 >  1:1137294/1‑139 (MQ=255)

         gAACTTCCTTTTCTTGCCTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATACAGTGGCTGCAACTGt                                                                                                                        <  2:323171/139‑1 (MQ=255)

         gAACTTCCTTTTCTTGCCTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATACAGTGGCTGCAACTGt                                                                                                                        <  2:235918/139‑1 (MQ=255)

         gAACTTCCTTTTCTTGCCTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATACAGTGGCTGCAACTGt                                                                                                                        >  2:208650/1‑139 (MQ=255)

              tCCTTTTCTTGCCTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATACAGTGGCTGCAACTGTCCAcc                                                                                                                   <  2:1054098/139‑1 (MQ=255)

                 ttctCTTGCCTCGTGTTTGCTAACAGAGAAGTACCAGAACCCGAAAACAAGATTGCTGCTTCTATCCACCGGACAAATCCGCGTCGAGTGAAGGACATCTGATTATCCCCTAATACAGTGGCTGAAACTGTCCACCCTa                                                                                                                >  1:904021/4‑139 (MQ=255)

                         ccTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATACAGTGGCTGCAACTGTc                                                                                                                       >  2:543213/1‑124 (MQ=255)

                         ccTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATACAGTGGCTGCAACTGTc                                                                                                                       <  1:543213/124‑1 (MQ=255)

                          cTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATACAGTGGCTGCAACTGTCCACCCTATCCATGACa                                                                                                       <  1:208650/139‑1 (MQ=255)

                            cGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATACAGTGGCTGCAACTGTCCACCCTATCCATGACAtc                                                                                                     >  2:389128/1‑139 (MQ=255)

                              tgtTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATACAGTGGCTGCAACTGTCCAcc                                                                                                                   >  2:731539/1‑123 (MQ=255)

                              tgtTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATACAGTGGCTGCAACTGTCCAcc                                                                                                                   <  1:731539/123‑1 (MQ=255)

                              tgtTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATACAGTGGCTGCAACTGTCCACCCTATCCATGACAtctc                                                                                                   >  2:1062095/1‑139 (MQ=255)

                                  cGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATACAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTg                                                                                               >  2:696242/1‑139 (MQ=255)

                                        cAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACGTCTGATTATCCCCTAATACAGTGGCTGCAACTGTCCACCCTa                                                                                                                <  2:770536/116‑1 (MQ=255)

                                        cAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCAAGTGAAGGACATCTGATTATCCCCTAATACAGTGGCTGCAACTGTCCACCCTa                                                                                                                >  1:770536/1‑116 (MQ=255)

                                             aagaCGCAGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATACAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAggg                                                                                    >  2:594210/1‑139 (MQ=255)

                                              agaCGCAGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATACAGTGGCTGCAACTGTcc                                                                                                                      <  1:434195/104‑1 (MQ=255)

                                              agaCGCAGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATACAGTGGCTGCAACTGTcc                                                                                                                      >  2:434195/1‑104 (MQ=255)

                                                   cAGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATACAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTg                                                                              >  2:489791/1‑139 (MQ=255)

                                                    aGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATACAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGa                                                                             <  1:696242/139‑1 (MQ=255)

                                                     gAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATACAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGaa                                                                            >  1:1171459/1‑139 (MQ=255)

                                                      aaCCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATACAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGaaa                                                                           <  2:613817/139‑1 (MQ=255)

                                                           gTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATACAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAgg                                                                                     >  1:168658/1‑124 (MQ=255)

                                                           gTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATACAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAgg                                                                                     <  2:168658/124‑1 (MQ=255)

                                                           gTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATACAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGct                                                                      <  2:1137294/139‑1 (MQ=255)

                                                                    aTTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATACAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGa                                                             >  2:1063995/1‑139 (MQ=255)

                                                                             cccATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATACAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCa                                                                                       <  1:360619/104‑1 (MQ=255)

                                                                             cccATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATACAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCa                                                                                       >  2:360619/1‑104 (MQ=255)

                                                                             cccATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATACAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGc                                                    >  1:980286/1‑139 (MQ=255)

                                                                             cccATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATACAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGc                                                    >  1:281501/1‑139 (MQ=255)

                                                                               cATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATACAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGcat                                                  <  1:594210/139‑1 (MQ=255)

                                                                                aTCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATACAGTGGCTGCAACTGTCCAcc                                                                                                                   <  2:149747/73‑1 (MQ=255)

                                                                                aTCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATACAGTGGCTGCAACTGTCCAcc                                                                                                                   >  1:149747/1‑73 (MQ=255)

                                                                                aTCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATACAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGcata                                                 >  2:842816/1‑139 (MQ=255)

                                                                                          cAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATACAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGcataagccataagc                                       <  2:281501/139‑1 (MQ=255)

                                                                                             aTTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATACAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGct                                                                      <  1:568178/105‑1 (MQ=255)

                                                                                             aTTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATACAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGct                                                                      >  2:568178/1‑105 (MQ=255)

                                                                                             aTTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATACAGTGGCTGCAACTGTCCAcc                                                                                                                   <  2:1045529/60‑1 (MQ=255)

                                                                                             aTTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATACAGTGGCTGCAACTGTCCAcc                                                                                                                   >  1:1045529/1‑60 (MQ=255)

                                                                                                  cGTCGAGTGAAGGACATCTGATTATCCCCTAATACAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCAt                               <  1:389128/139‑1 (MQ=255)

                                                                                                  cGTCGAGTGAAGGACATCTGATTATCCCCTAATACAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCAt                               <  1:153113/139‑1 (MQ=255)

                                                                                                  cGTCGAGTGAAGGACATCTGATTATCCCCTAATACAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCAt                               <  1:267624/139‑1 (MQ=255)

                                                                                                                cATCTGATTATCCCCTAATACAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCAt                               <  2:451346/125‑1 (MQ=255)

                                                                                                                cATCTGATTATCCCCTAATACAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCAt                               >  1:451346/1‑125 (MQ=255)

                                                                                                                   cTGATTATCCCCTAATACAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGaaa                                                                           <  2:225711/78‑1 (MQ=255)

                                                                                                                   cTGATTATCCCCTAATACAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGaaa                                                                           >  1:225711/1‑78 (MQ=255)

                                                                                                                   cTGATTATCCCCTAATACAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAAtcgctcg              >  2:709380/1‑139 (MQ=255)

                                                                                                                          tCCCCTAATACAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACa       >  2:917919/1‑139 (MQ=255)

                                                                                                                               tAATACAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTac  <  1:917919/139‑1 (MQ=255)

TGGCATACCGAACTTCCTTTTCTTGCCTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTAC  >  NC_000913/1750899‑1751164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A