Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I1 R1	63	64.8	2934156	93.7%	2749304	111.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,756,208	G→A	A171T (GCT→ACT)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,756,208	0	G	A	100.0%	142.3 / NA	41	A171T (GCT→ACT)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/20); total (21/20)

TGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCC  >  NC_000913/1756072‑1756343

tGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACt                                                                                                                                       >  1:1030775/1‑139 (MQ=255)

  aCGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCActgactga                                                                                                                                     <  1:1270980/139‑1 (MQ=255)

  aCGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCGGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCActgactga                                                                                                                                     <  2:1324063/139‑1 (MQ=255)

   cGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGaa                                                                                                                                    >  1:517000/1‑139 (MQ=255)

        gTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGa                                                                                                                               <  1:732807/139‑1 (MQ=255)

                  tATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGAc                                                                                                                     >  2:1457850/1‑139 (MQ=255)

                  tATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGAc                                                                                                                     <  2:1030775/139‑1 (MQ=255)

                            aCCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGa                                                                                                                               <  1:966928/119‑1 (MQ=255)

                            aCCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCtt                                                                                                             <  2:274542/137‑1 (MQ=255)

                            aCCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCtt                                                                                                             >  1:274542/1‑137 (MQ=255)

                            aCCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTg                                                                                                           >  2:807272/1‑139 (MQ=255)

                            aCCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGCGCGAAAACAAAGGTGTGAACCTGCCTGGCATTTCCATTGCTCTGCCAGCACTGACTGAAAgaga                                                                                                                               >  2:966928/1‑119 (MQ=255)

                                   ttGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAAc                                                                                                                          >  2:1415590/1‑117 (MQ=255)

                                   ttGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAAc                                                                                                                          <  1:1415590/117‑1 (MQ=255)

                                     gAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAAc                                                                                                  <  2:1261198/139‑1 (MQ=255)

                                           aaCAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCg                                                                                            <  2:1308426/139‑1 (MQ=255)

                                             cAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTa                                                                                          <  1:708658/139‑1 (MQ=255)

                                               aaGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGa                                                                                        <  1:986788/139‑1 (MQ=255)

                                                    aTCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTttg                                                                                   <  1:866938/139‑1 (MQ=255)

                                                              tGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTc                                                                         >  2:472839/1‑139 (MQ=255)

                                                                cTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAAc                                                                                                  >  1:1140811/1‑112 (MQ=255)

                                                                cTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAAc                                                                                                  <  2:1140811/112‑1 (MQ=255)

                                                                cTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTgctgct                                                                           >  1:1150316/1‑135 (MQ=255)

                                                                cTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTgctgct                                                                           <  2:1150316/135‑1 (MQ=255)

                                                                   aacaacGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTa                                                                    >  2:1258119/1‑139 (MQ=255)

                                                                        cGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGt                                                               >  1:76414/1‑139 (MQ=255)

                                                                             aCCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGAc                                                                                                                     >  2:120441/1‑80 (MQ=255)

                                                                             aCCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGAc                                                                                                                     <  1:120441/80‑1 (MQ=255)

                                                                                  ggCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAAc                                                                                                  >  1:1409262/1‑94 (MQ=255)

                                                                                  ggCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAAc                                                                                                  <  2:1409262/94‑1 (MQ=255)

                                                                                  ggCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGt                                                               <  1:421489/129‑1 (MQ=255)

                                                                                  ggCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGt                                                               >  2:421489/1‑129 (MQ=255)

                                                                                     gAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACg                                                  <  1:321750/139‑1 (MQ=255)

                                                                                                  tgAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACaaa                                                                                                                           >  2:471912/1‑53 (MQ=255)

                                                                                                  tgAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACaaa                                                                                                                           <  1:471912/53‑1 (MQ=255)

                                                                                                   gAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGt                                    >  1:1403665/1‑139 (MQ=255)

                                                                                                         gcctgGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCAc                              >  1:572218/1‑139 (MQ=255)

                                                                                                         gcctgGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCAc                              >  2:679475/1‑139 (MQ=255)

                                                                                                                   tCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGAcc                                                                                                                    <  1:1301716/43‑1 (MQ=255)

                                                                                                                   tCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGAcc                                                                                                                    >  2:1301716/1‑43 (MQ=255)

                                                                                                                               ccAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTCGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGaa         <  2:866695/138‑1 (MQ=255)

                                                                                                                               ccAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGaaa        <  1:1258119/139‑1 (MQ=255)

                                                                                                                               ccAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGaa         >  1:866695/1‑138 (MQ=255)

                                                                                                                                     ctgactgaAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATcc  <  1:1457850/139‑1 (MQ=255)

TGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCC  >  NC_000913/1756072‑1756343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A