Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F1 I1 R1
|
65 |
25.3 |
1027584 |
98.0% |
1007032 |
120.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,756,216 |
(A)5→4 |
coding (519/1413 nt) |
pykF → |
pyruvate kinase I |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,756,212 | 0 | A | . | 100.0%
| 93.5
/ NA
| 22 | coding (515/1413 nt) | pykF | pyruvate kinase I |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (11/11); total (11/11) |
CGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCC > NC_000913/1756075‑1756343
|
cGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGaa > 1:213702/1‑139 (MQ=255)
gTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGAc < 2:310216/139‑1 (MQ=255)
tATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGAcc > 2:172809/1‑139 (MQ=255)
aCCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTCTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTgg > 1:421149/1‑139 (MQ=255)
aCCGCCATTGAAGGAAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGCACCTGCCTGGCGTTTCCATTGCTCTGACAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTgg > 2:312340/1‑139 (MQ=255)
cGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGtt < 1:392028/139‑1 (MQ=255)
ccATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGAcc < 2:471405/125‑1 (MQ=255)
ccATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGAcc > 1:471405/1‑125 (MQ=255)
tGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAAc < 2:460312/139‑1 (MQ=255)
aaCAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGATAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGt > 2:309955/1‑139 (MQ=255)
cAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAg > 1:148300/1‑139 (MQ=255)
aTCTATAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTttgt < 2:86353/139‑1 (MQ=255)
cTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTttgttg < 1:427151/139‑1 (MQ=255)
gTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTtgct < 2:213702/139‑1 (MQ=255)
aGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCtt > 1:108236/1‑139 (MQ=255)
ggCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGa > 2:344793/1‑139 (MQ=255)
ggCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGa > 2:73495/1‑139 (MQ=255)
gCGAAAACTAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGAc < 1:312340/139‑1 (MQ=255)
cGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACg < 1:344793/139‑1 (MQ=255)
ggTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAAt < 1:421753/139‑1 (MQ=255)
tgGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGa > 1:394871/1‑139 (MQ=255)
gCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGaaa < 2:394871/139‑1 (MQ=255)
aCTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATcc > 2:327408/1‑139 (MQ=255)
|
CGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCC > NC_000913/1756075‑1756343
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
