Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F1 I2 R1
|
69 |
53.0 |
2252012 |
96.1% |
2164183 |
115.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,756,216 |
(A)5→4 |
coding (519/1413 nt) |
pykF → |
pyruvate kinase I |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,756,212 | 0 | A | . | 100.0%
| 156.6
/ NA
| 38 | coding (515/1413 nt) | pykF | pyruvate kinase I |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (21/17); total (21/17) |
GTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACA > NC_000913/1756080‑1756346
|
gTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGaaaagac > 1:53233/1‑136 (MQ=255)
gATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGCCCTGGGCGAAAACAAAGGTGTGACCCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAAc < 1:109556/139‑1 (MQ=255)
aaGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGAcc < 2:445309/134‑1 (MQ=255)
aaGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGAcc > 1:445309/1‑134 (MQ=255)
aCCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTgg > 1:16312/1‑139 (MQ=255)
aCCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTgg > 1:398585/1‑139 (MQ=255)
tGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAAc < 1:827445/139‑1 (MQ=255)
aaCAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGAcc < 2:527912/114‑1 (MQ=255)
aaCAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGAcc > 1:527912/1‑114 (MQ=255)
cAAAGTTAACTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAg > 1:731810/1‑139 (MQ=255)
tGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTtgc < 2:431548/139‑1 (MQ=255)
tAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTtgctg < 2:394020/139‑1 (MQ=255)
tAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTtgctg < 1:968672/139‑1 (MQ=255)
aaGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTgctgct > 2:992655/1‑139 (MQ=255)
aGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGAcc < 1:1017991/97‑1 (MQ=255)
aGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGAcc > 2:1017991/1‑97 (MQ=255)
aacaacGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTAt > 1:567327/1‑139 (MQ=255)
cGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAg > 1:632009/1‑81 (MQ=255)
cGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAg < 2:632009/81‑1 (MQ=255)
cGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAAc < 2:895584/103‑1 (MQ=255)
cGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAAc > 1:895584/1‑103 (MQ=255)
aCCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGt > 2:300333/1‑133 (MQ=255)
aCCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGt < 1:300333/133‑1 (MQ=255)
aCCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGt > 2:458812/1‑139 (MQ=255)
ggCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACa < 2:315490/94‑1 (MQ=255)
ggCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACa > 1:315490/1‑94 (MQ=255)
ggCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGa > 1:609678/1‑139 (MQ=255)
cGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACg < 2:936905/139‑1 (MQ=255)
aaCAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTAt < 2:53233/139‑1 (MQ=255)
cctgGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCt > 2:1804/1‑139 (MQ=255)
tCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCa > 2:736888/1‑139 (MQ=255)
tGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTtgctgc < 2:766114/77‑1 (MQ=255)
tGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTtgctgc > 1:766114/1‑77 (MQ=255)
tGCCAGCACTGGCTG‑AAATGACCAAGAGGACCTGATGTTTGGTTTTGAACAAGGCGTAGATTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGGTATAGAAATCCGTGAGCACCTGAAAGAGCATGGCGGCGaa > 2:957128/1‑139 (MQ=255)
ccAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTaa > 2:459223/1‑85 (MQ=255)
ccAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTaa < 1:459223/85‑1 (MQ=255)
ccAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGaaaa < 2:609678/139‑1 (MQ=255)
ggCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCcaca > 2:147113/1‑139 (MQ=255)
ggCTG‑AAAAGACAAACAGAACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCcaca > 2:625000/1‑139 (MQ=255)
|
GTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACA > NC_000913/1756080‑1756346
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
