Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I2 R1	92	50.6	2362856	94.3%	2228173	111.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,203,362	A→G	I1028M (ATA→ATG)	yehI →	DUF4132 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,203,362	0	A	G	100.0%	189.6 / NA	50	I1028M (ATA→ATG)	yehI	DUF4132 domain‑containing protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (27/23); total (27/23)

ATGCTACTCAGGTGAGCAAAAACCTGCTCCCGCGTCTTGAAACGGCCCTACGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATATGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGCCCACC  >  NC_000913/2203226‑2203495

aTGCTACTCAGGTGAGCAAAAACCTGCTCCCGCGTCTTGAAACGGCCCTACGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAAtgtg                                                                                                                                     <  1:1110500/139‑1 (MQ=255)

aTGCTACTCAGGTGAGCAAAAACCTGCTCCCGCGTCTTGAAACGGCCCTACGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTCGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAAtgtg                                                                                                                                     <  1:831483/139‑1 (MQ=255)

             gAGCAAAAACCTGCTCCCGCGTCTTGAAACGGCCCTACGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCg                                                                                                                        >  2:777229/1‑139 (MQ=255)

              aGCAAAAACCTGCTCCCGCGTCTTGAAACGGCCCTACGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCgg                                                                                                                       >  2:593967/1‑139 (MQ=255)

              aGCAAAAACCTGCTCCCGCGTCTTGAAACGGCCCTACGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCgg                                                                                                                       >  1:45189/1‑139 (MQ=255)

              aGCAAAAACCTGCTCCCGCGTCTTGAAACGGCCCTACGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCgg                                                                                                                       >  2:409258/1‑139 (MQ=255)

                cAAAAACCTGCTCCCGCGTCTTGAAACGGCCCTACGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCa                                                                                                                     >  1:449556/1‑139 (MQ=255)

                   aaaCCTGCTCCCGCGTCTTGAAACGGCCCTACGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAAt                                                                                                                  >  2:25286/1‑139 (MQ=255)

                     aCCTGCTCCCGCGTCTTGAAACGGCCCTACGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGa                                                                                                                >  2:185333/1‑139 (MQ=255)

                         gCTCCCGCGTCTTGAAACGGCCCTACGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACcg                                                                                                            >  1:688489/1‑139 (MQ=255)

                          cTCCCGCGTCTTGAAACGGCCCTACGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTTCCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACcgc                                                                                                           >  1:797374/1‑139 (MQ=255)

                          cTCCCGCGTCTTGAAACGGCCCTACGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACcgc                                                                                                           <  1:25286/139‑1 (MQ=255)

                          cTCCCGCGTCTTGAAACGGCCCTACGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACcgc                                                                                                           >  1:1091591/1‑139 (MQ=255)

                          cTCCCGCGTCTTGAAACGGCCCTACGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACcgc                                                                                                           <  2:45189/139‑1 (MQ=255)

                                       aaaCGGCCCTACGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCaa                                                                                              <  1:593967/139‑1 (MQ=255)

                                                      ccaccCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGc                                                                               <  2:153735/139‑1 (MQ=255)

                                                      ccaccCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGc                                                                               <  2:16797/139‑1 (MQ=255)

                                                            gACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCaga                                                                         >  2:972524/1‑139 (MQ=255)

                                                            gACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCaga                                                                         >  1:538567/1‑139 (MQ=255)

                                                                cTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAgggg                                                                     <  2:551906/139‑1 (MQ=255)

                                                                cTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAgggg                                                                     <  2:1091591/139‑1 (MQ=255)

                                                                cTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAgggg                                                                     <  1:409258/139‑1 (MQ=255)

                                                                   gTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGc                                                                            >  2:67456/1‑129 (MQ=255)

                                                                   gTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGc                                                                            <  1:67456/129‑1 (MQ=255)

                                                                                      tctcTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATg                                               <  2:538567/139‑1 (MQ=255)

                                                                                                    tcatcCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGAcctg                                 >  1:662182/1‑139 (MQ=255)

                                                                                                      atcCCTTTACCCGTCTGGTTACCCAGCGATTAAtgtg                                                                                                                                     >  2:189475/1‑37 (MQ=255)

                                                                                                            ttACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGa                         >  2:29114/1‑139 (MQ=255)

                                                                                                             tACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGAc                        >  1:519652/1‑139 (MQ=255)

                                                                                                                ccGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGct                     <  2:743706/139‑1 (MQ=255)

                                                                                                                ccGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGct                     <  2:797374/139‑1 (MQ=255)

                                                                                                                  gTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCag                                                                          <  1:518178/84‑1 (MQ=255)

                                                                                                                  gTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCag                                                                          >  2:518178/1‑84 (MQ=255)

                                                                                                                  gTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGctct                   >  1:962730/1‑139 (MQ=255)

                                                                                                                   tCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAgggg                                                                     >  1:13153/1‑88 (MQ=255)

                                                                                                                   tCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAgggg                                                                     <  2:13153/88‑1 (MQ=255)

                                                                                                                   tCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTg                  >  1:491428/1‑139 (MQ=255)

                                                                                                                       gTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTg              >  2:1108169/1‑139 (MQ=255)

                                                                                                                        ttACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTgg             <  2:491428/139‑1 (MQ=255)

                                                                                                                         tACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGct                     >  2:210549/1‑130 (MQ=255)

                                                                                                                         tACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGct                     <  1:210549/130‑1 (MQ=255)

                                                                                                                              aGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAgggg                                                                     <  1:385462/77‑1 (MQ=255)

                                                                                                                              aGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAgggg                                                                     >  2:385462/1‑77 (MQ=255)

                                                                                                                               gCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGcc      <  2:527914/139‑1 (MQ=255)

                                                                                                                               gCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGcc      <  2:449556/139‑1 (MQ=255)

                                                                                                                                cGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGccc     >  2:520434/1‑139 (MQ=255)

                                                                                                                                 gATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCg                                                                             >  2:741322/1‑66 (MQ=255)

                                                                                                                                 gATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCg                                                                             <  1:741322/66‑1 (MQ=255)

                                                                                                                                 gATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACg                       >  1:172146/1‑120 (MQ=255)

                                                                                                                                 gATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACg                       <  2:172146/120‑1 (MQ=255)

                                                                                                                                   ttAATGTGGGGGGTTTATCCGGCAAATGAACcgcg                                                                                                          >  1:806562/1‑35 (MQ=255)

                                                                                                                                   ttAATGTGGGGGGTTTATCCGGCAAATGAACcgcg                                                                                                          <  2:806562/35‑1 (MQ=255)

                                                                                                                                   ttAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGCCCAcc  <  2:662182/139‑1 (MQ=255)

ATGCTACTCAGGTGAGCAAAAACCTGCTCCCGCGTCTTGAAACGGCCCTACGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATATGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGCCCACC  >  NC_000913/2203226‑2203495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A