Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I2 R1	92	50.6	2362856	94.3%	2228173	111.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,489,435	G→A	A251V (GCA→GTA)	yfdV ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,489,435	0	G	A	100.0%	155.5 / NA	45	A251V (GCA→GTA)	yfdV	putative transporter
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (20/25); total (20/25)

CTCACAGCCAATGACGCTGTACCAGTACGGGTGTAGACATTAAACCGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCT  >  NC_000913/2489303‑2489557

cTCACAGCCAATGACGCTGTACCAGTACGGGTGTAGACATTAAACCGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGAcataccatac                                                                                                                      <  1:429359/139‑1 (MQ=255)

cTCACAGCCAATGACGCTGTACCAGTACGGGTGTAGACATTAAACCGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGAcataccatac                                                                                                                      >  2:687883/1‑139 (MQ=255)

 tCACAGCCAATGACGCTGTACCAGTACGGGTGTAGACATTAAACCGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACataccatacc                                                                                                                     >  1:523057/1‑139 (MQ=255)

     aGCCAATGACGCTGTACCAGTACGGGTGTAGACATTAAACCGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACg                                                                                                                 <  1:1092585/139‑1 (MQ=255)

                     ccAGTACGGGTGTAGACATTAAACCGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTg                                                                                                 <  2:287787/139‑1 (MQ=255)

                     ccAGTACGGGTGTAGACATTAAACCGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTg                                                                                                 <  2:403547/139‑1 (MQ=255)

                         tACGGGTGTAGACATTAAACCGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCAt                                                                                             <  2:782549/139‑1 (MQ=255)

                             ggTGTAGACATTAAACCGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGa                                                                                         >  1:101200/1‑139 (MQ=255)

                             ggTGTAGACATTAAACCGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGa                                                                                         >  2:811184/1‑139 (MQ=255)

                              gtgtAGACATTAAACCGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGaa                                                                                        <  2:101200/139‑1 (MQ=255)

                                  aGACATTAAACCGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAg                                                                                    <  2:992804/139‑1 (MQ=255)

                                  aGACATTAAACCGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAg                                                                                    <  1:687883/139‑1 (MQ=255)

                                          aaCCGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGa                                                                            <  2:349694/139‑1 (MQ=255)

                                               gCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGc                                                                                                           >  1:137516/1‑103 (MQ=255)

                                               gCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGc                                                                                                           <  2:137516/103‑1 (MQ=255)

                                                cTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGgtgt                                                                      >  1:1151999/1‑139 (MQ=255)

                                                 tGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGtt                                                                     <  1:936655/139‑1 (MQ=255)

                                                                 ggAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATc                                                                                      <  1:1058488/106‑1 (MQ=255)

                                                                 ggAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATc                                                                                      >  2:1058488/1‑106 (MQ=255)

                                                                 ggAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCa                                                     <  2:35896/139‑1 (MQ=255)

                                                                       tGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAAc                                               >  1:127587/1‑139 (MQ=255)

                                                                          cggcggTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCg                                            >  2:595039/1‑139 (MQ=255)

                                                                                tAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTa                                      <  2:263/139‑1 (MQ=255)

                                                                                 aaTGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTAt                                     >  2:564351/1‑139 (MQ=255)

                                                                                  aTGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGaa                                                                           <  1:695432/100‑1 (MQ=255)

                                                                                  aTGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGaa                                                                           >  2:695432/1‑100 (MQ=255)

                                                                                  aTGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAg                                                                          <  1:56831/101‑1 (MQ=255)

                                                                                  aTGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAg                                                                          >  2:56831/1‑101 (MQ=255)

                                                                                         tGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGcc                             <  1:595039/139‑1 (MQ=255)

                                                                                          gCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCa                            >  1:653440/1‑139 (MQ=255)

                                                                                                  catcatCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAg                                                                          <  1:323443/85‑1 (MQ=255)

                                                                                                  catcatCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAg                                                                          >  2:323443/1‑85 (MQ=255)

                                                                                                         tGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGt                                                                                                  >  1:746800/1‑54 (MQ=255)

                                                                                                         tGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGt                                                                                                  <  2:746800/54‑1 (MQ=255)

                                                                                                           cAGATGTTCGCTGTTCAAATGACATACCATACCAACga                                                                                                                >  1:296650/1‑38 (MQ=255)

                                                                                                           cAGATGTTCGCTGTTCAAATGACATACCATACCAACga                                                                                                                <  2:296650/38‑1 (MQ=255)

                                                                                                              aTGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAg                                                                          >  2:116378/1‑73 (MQ=255)

                                                                                                              aTGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAg                                                                          <  1:116378/73‑1 (MQ=255)

                                                                                                              aTGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAAt        >  2:254757/1‑139 (MQ=255)

                                                                                                                gTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAAt                                                                                       <  2:1136093/58‑1 (MQ=255)

                                                                                                                gTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAAt                                                                                       >  1:1136093/1‑58 (MQ=255)

                                                                                                                  tCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCg    <  1:254757/139‑1 (MQ=255)

                                                                                                                  tCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCg    >  2:1129701/1‑139 (MQ=255)

                                                                                                                   cGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCtt                                                                                 <  2:68912/61‑1 (MQ=255)

                                                                                                                   cGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCtt                                                                                 >  1:68912/1‑61 (MQ=255)

                                                                                                                    gCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCt  <  2:653440/139‑1 (MQ=255)

                                                                                                                     cTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGt                                                                                                  <  2:253331/42‑1 (MQ=255)

                                                                                                                     cTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGt                                                                                                  >  1:253331/1‑42 (MQ=255)

CTCACAGCCAATGACGCTGTACCAGTACGGGTGTAGACATTAAACCGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCT  >  NC_000913/2489303‑2489557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A