Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I2 R1	92	50.6	2362856	94.3%	2228173	111.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,007,295	T→C	V345A (GTG→GCG)	ygeW →	putative carbamoyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,007,295	0	T	C	100.0%	186.8 / NA	49	V345A (GTG→GCG)	ygeW	putative carbamoyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (23/26); total (23/26)

AACGCGCAACACAAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAG  >  NC_000913/3007171‑3007429

aaCGCGCAACACAAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTAt                                                                                                                          >  1:1646/1‑139 (MQ=255)

aaCGCGCAACACAAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTAt                                                                                                                          >  1:866204/1‑139 (MQ=255)

          acaAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATa                                                                                                                <  1:1016015/139‑1 (MQ=255)

                  tGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCg                                                                                                        <  2:1097744/139‑1 (MQ=255)

                   ggCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGaa                                                                                                                     <  2:870243/125‑1 (MQ=255)

                   ggCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGaa                                                                                                                     >  1:870243/1‑125 (MQ=255)

                   ggCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTa                                                                                                           >  2:404839/1‑135 (MQ=255)

                   ggCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTa                                                                                                           <  1:404839/135‑1 (MQ=255)

                   ggCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGc                                                                                                       >  1:1067562/1‑139 (MQ=255)

                   ggCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGc                                                                                                       <  2:1646/139‑1 (MQ=255)

                            cTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACaa                                                                                              >  2:672910/1‑139 (MQ=255)

                              gAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTa                                                                                                           >  2:92675/1‑124 (MQ=255)

                              gAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTa                                                                                                           <  1:92675/124‑1 (MQ=255)

                                  agaTGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGc                                                                                        <  1:429143/139‑1 (MQ=255)

                                  agaTGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGc                                                                                        <  2:888335/139‑1 (MQ=255)

                                    atgatgGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAgg                                                                                                                               >  2:426235/1‑98 (MQ=255)

                                    atgatgGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAgg                                                                                                                               <  1:426235/98‑1 (MQ=255)

                                    atgatgGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCa                                                                                      >  2:1019946/1‑139 (MQ=255)

                                      gatgGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGc                                                                                    >  1:1166317/1‑139 (MQ=255)

                                         ggAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTgg                                                                                 <  1:415063/139‑1 (MQ=255)

                                         ggAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTgg                                                                                 <  2:866204/139‑1 (MQ=255)

                                         ggAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTgg                                                                                 >  2:748747/1‑139 (MQ=255)

                                              tGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGc                                                                                                       >  1:531919/1‑112 (MQ=255)

                                              tGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGc                                                                                                       <  2:531919/112‑1 (MQ=255)

                                                             aaGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTgg                                                                                 <  2:296590/119‑1 (MQ=255)

                                                             aaGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTgg                                                                                 >  1:296590/1‑119 (MQ=255)

                                                                    gTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATc                                                      <  1:672910/139‑1 (MQ=255)

                                                                    gTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATc                                                      <  2:1166317/139‑1 (MQ=255)

                                                                          gCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCg                                                                                                        >  1:568767/1‑83 (MQ=255)

                                                                          gCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCg                                                                                                        <  2:568767/83‑1 (MQ=255)

                                                                               gcctgccAGCTGATATCAGCGGCGTATCGTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAAAACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTaa                                           >  2:730764/1‑139 (MQ=255)

                                                                               gcctgccAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGaaaa                                                                                                                   >  2:660165/1‑67 (MQ=255)

                                                                               gcctgccAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGaaaa                                                                                                                   <  1:660165/67‑1 (MQ=255)

                                                                               gcctgccAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTgg                                                                                 >  2:1170060/1‑101 (MQ=255)

                                                                               gcctgccAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTgg                                                                                 <  1:1170060/101‑1 (MQ=255)

                                                                               gcctgccAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTaa                                           >  1:1164630/1‑139 (MQ=255)

                                                                               gcctgccAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTaa                                           >  2:273940/1‑139 (MQ=255)

                                                                                        cTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCaa                                  <  2:1164630/139‑1 (MQ=255)

                                                                                           atatCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAAcc                               <  1:955955/139‑1 (MQ=255)

                                                                                                       tATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTa                                                                                                           >  1:872709/1‑51 (MQ=255)

                                                                                                       tATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTa                                                                                                           <  2:872709/51‑1 (MQ=255)

                                                                                                              tAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAATCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCaa            <  1:745443/139‑1 (MQ=255)

                                                                                                              tAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCaa            <  1:273940/139‑1 (MQ=255)

                                                                                                              tAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCaa            >  2:745443/1‑139 (MQ=255)

                                                                                                                aagaagGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAAct          <  2:1067562/139‑1 (MQ=255)

                                                                                                                   aagGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAActgct       >  1:371700/1‑139 (MQ=255)

                                                                                                                        gAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCt                   <  2:288968/122‑1 (MQ=255)

                                                                                                                        gAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCt                   >  1:288968/1‑122 (MQ=255)

                                                                                                                        gAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAaag  <  1:309715/139‑1 (MQ=255)

AACGCGCAACACAAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAG  >  NC_000913/3007171‑3007429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A