Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I2 R1	92	50.6	2362856	94.3%	2228173	111.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,142,833	C→T	A53T (GCA→ACA)	hybC ←	hydrogenase 2, large subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,142,833	0	C	T	100.0%	106.6 / NA	31	A53T (GCA→ACA)	hybC	hydrogenase 2, large subunit
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (20/11); total (20/11)

AATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCGGAT  >  NC_000913/3142705‑3142967

aaTGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGTATCGCGCGGa                                                                                                                              >  2:389883/1‑139 (MQ=255)

          cGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGTATCGCGCGGATCGCGGtttt                                                                                                                    >  1:820476/1‑139 (MQ=255)

          cGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGTATCGCGCGGATCGCGGtttt                                                                                                                    >  2:206093/1‑139 (MQ=255)

             aTGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGTATCGCGCGGATCGCGGTTTTTCa                                                                                                                 <  2:456914/139‑1 (MQ=255)

               gTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGTATCGCGCGGATCGCGGTTTTTCACg                                                                                                               <  1:389883/139‑1 (MQ=255)

               gTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGTATCGCGCGGATCGCGGTTTTTCACg                                                                                                               >  2:1025616/1‑139 (MQ=255)

                 aTTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGTATCGCGCGGATCGCGGTTTTTCACGAt                                                                                                             >  1:483540/1‑139 (MQ=255)

                 aTTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGTATCGCGCGGATCGCGGTTTTTCACGAt                                                                                                             >  2:901710/1‑139 (MQ=255)

                       cgTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGTATCGCGCGGATCGCGGTTTTTCACGATCTCTTc                                                                                                       >  2:873403/1‑139 (MQ=255)

                          tAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGTATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCAt                                                                                                    >  2:670384/1‑139 (MQ=255)

                                     tCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGTATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCcaca                                                                                         <  2:483540/139‑1 (MQ=255)

                                                cgcACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGTATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGaa                                                                              >  2:488257/1‑139 (MQ=255)

                                                                   aCGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGTATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAacga                                                           >  1:646365/1‑139 (MQ=255)

                                                                              cgcgTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGTATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCg                                                >  1:348461/1‑139 (MQ=255)

                                                                              cgcgTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGTATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCg                                                >  2:615796/1‑139 (MQ=255)

                                                                               gcgTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGTATCGCGCGGATCGCGGTTTTTCACGAt                                                                                                             <  1:1030202/77‑1 (MQ=255)

                                                                               gcgTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGTATCGCGCGGATCGCGGTTTTTCACGAt                                                                                                             >  2:1030202/1‑77 (MQ=255)

                                                                                cgTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGTATCGCGCGGATCGCGGt                                                                                                                       <  1:844547/66‑1 (MQ=255)

                                                                                cgTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGTATCGCGCGGATCGCGGt                                                                                                                       >  2:844547/1‑66 (MQ=255)

                                                                                  tGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGTATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGAttt                                            <  2:348461/139‑1 (MQ=255)

                                                                                           gtaCATACGCCACAGATACGTTGCACAATCATCCATGTATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCGa                                   <  2:820476/139‑1 (MQ=255)

                                                                                              cATACGCCACAGATACGTTGCACAATCATCCATGTATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGc                                >  2:508868/1‑139 (MQ=255)

                                                                                                 aCGCCACAGATACGTTGCACAATCATCCATGTATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGa                                               >  1:1100650/1‑121 (MQ=255)

                                                                                                 aCGCCACAGATACGTTGCACAATCATCCATGTATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGa                                               <  2:1100650/121‑1 (MQ=255)

                                                                                                   gCCACAGATACGTTGCACAATCATCCATGTATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTaaa                           >  1:643036/1‑139 (MQ=255)

                                                                                                      acaGATACGTTGCACAATCATCCATGTATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGc                        >  2:1008358/1‑139 (MQ=255)

                                                                                                        aGATACGTTGCACAATCATCCATGTATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGccc                      <  1:670384/139‑1 (MQ=255)

                                                                                                          aTACGTTGCACAATCATCCATGTATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCt                    <  1:488257/139‑1 (MQ=255)

                                                                                                                          tcCATGTATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCgg    >  2:1039895/1‑139 (MQ=255)

                                                                                                                          tcCATGTATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCgg    <  1:873403/139‑1 (MQ=255)

                                                                                                                            cATGTATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCGGAt  >  2:193807/1‑139 (MQ=255)

AATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCGGAT  >  NC_000913/3142705‑3142967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A