Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I2 R1	67	36.0	1614110	95.1%	1535018	114.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,159,844	G→A	P435S (CCA→TCA)	ygiQ ←	Radical SAM superfamily protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,159,844	0	G	A	100.0%	145.2 / NA	43	P435S (CCA→TCA)	ygiQ	Radical SAM superfamily protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/22); total (21/22)

GATAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACGCACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGGCCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGAACGGCTCTGAATAATGCGCCCTTCGTGCTCGGTGATAGAACAGAAA  >  NC_000913/3159712‑3159974

gATAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACGCACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACccacc                                                                                                                              <  2:80661/139‑1 (MQ=255)

                 ggttcgtggttcgtGTCCATGTGCGGACAAATATCCGGATAAACGCACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGATCCACCAAGATCGGAAATCACGc                                                                                                             >  2:116380/1‑139 (MQ=255)

                     cgtggttcgtgTCCATGTGCGGACAAATATCCGGATAAACGCACGACAAACGGCGACAAGTTTGTTCAGCGCAAGGCGATTTGCAGCGCCACATATACCTGTTGTCAGTTGACGCACCAAGATCTGAAATCACGCCAGt                                                                                                         >  2:260736/1‑139 (MQ=255)

                           tcgtgTCCATGTGCGGACAAATATCCGGATAAACGCACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAAcc                                                                                                   <  1:47123/139‑1 (MQ=255)

                              tgtCCATGTGCGGACAAATATCCGGATAAACGCACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTgg                                                                                                >  1:453709/1‑139 (MQ=255)

                                tCCATGTGCGGACAAATATCCGGATAAACGCACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGaa                                                                                              <  1:403637/139‑1 (MQ=255)

                                        cGGACAAATATCCGGATAAACGCACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTcg                                                                                      >  1:760266/1‑139 (MQ=255)

                                                      gATAAACGCACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGATGACCCACCAAGATCAGAAATCACGCACGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGAt                                                                        <  1:513462/139‑1 (MQ=255)

                                                       aTAAACGCACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAAcc                                                                                                   >  2:23757/1‑111 (MQ=255)

                                                       aTAAACGCACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAAcc                                                                                                   <  1:23757/111‑1 (MQ=255)

                                                       aTAAACGCACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGAtc                                                                       >  2:27128/1‑139 (MQ=255)

                                                         aaaCGCACGACAAACGCCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGAtctc                                                                     >  2:141737/1‑139 (MQ=255)

                                                                 gACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGa                                                             >  2:754221/1‑139 (MQ=255)

                                                                  aCAAACGGCGAGAAGTTTGTGTAGCGCGGGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACACACCAAGATCGTAAATCACGCCCGTAAAACCTGGAACGGTGTGGCGGATCGCTTCGATCTCATTAATGAt                                                            <  2:708444/139‑1 (MQ=255)

                                                                     aaCGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGa                                                         <  1:27128/139‑1 (MQ=255)

                                                                            aCAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCg                                                  >  2:280062/1‑139 (MQ=255)

                                                                               aGTTTGTTCAGCGCGTGGCGATTTGCATCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGaa                                               <  2:23183/139‑1 (MQ=255)

                                                                               aGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGaa                                               <  1:324612/139‑1 (MQ=255)

                                                                               aGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGaa                                               <  2:95266/139‑1 (MQ=255)

                                                                                   tGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGAACGGc                                           <  1:14391/139‑1 (MQ=255)

                                                                                          cgcgTGGCGATTTGTAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGAACGGCTCTGaat                                    >  1:491882/1‑139 (MQ=255)

                                                                                          cgcgTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTa                                                                 >  2:645763/1‑110 (MQ=255)

                                                                                          cgcgTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTa                                                                 <  1:645763/110‑1 (MQ=255)

                                                                                          cgcgTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGAACGGCTCTGaat                                    >  2:348429/1‑139 (MQ=255)

                                                                                          cgcgTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGAACGGCTCTGaat                                    >  1:316491/1‑139 (MQ=255)

                                                                                                gCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGAACGGCTCTGAATAATgcg                              <  1:280062/139‑1 (MQ=255)

                                                                                                gCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGAACGGCTCTGAATAATgcg                              <  1:394662/139‑1 (MQ=255)

                                                                                                            gcAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGTTCGAATCTTCGGAACGGCTCTGAATAATGCGCCCTTCGTGCTc                  <  1:754221/139‑1 (MQ=255)

                                                                                                            gcAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGAACGGCTCTGAATAATGCGCCCTTCGTGCTc                  <  1:348429/139‑1 (MQ=255)

                                                                                                              aaCATATACATGTTGGCAGTTGATCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGAACGGCTCTGAATAATGCGCCCTTCGTGCTCgg                <  1:116380/139‑1 (MQ=255)

                                                                                                                    taCATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGAACGGCTCTGAATAATGCGCCCTTCGTGCTCGGTGATAg          <  1:141737/139‑1 (MQ=255)

                                                                                                                          ttGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGaa                                                                                              >  2:445072/1‑49 (MQ=255)

                                                                                                                          ttGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGaa                                                                                              <  1:445072/49‑1 (MQ=255)

                                                                                                                          ttGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGAACGGCTCTGAATAATGCGCCCTTCGTGCTCGGTGATAGAACAGa    >  1:727051/1‑139 (MQ=255)

                                                                                                                           tGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGAACGGCTCTGAATAATGCGCCCTTCGTGCTCGGTGATAGAACAGaa   >  2:71753/1‑139 (MQ=255)

                                                                                                                            ggCAGTTGACCCACCAAGATCGGAAATCACGCCCGTaaa                                                                                                      >  1:748302/1‑39 (MQ=255)

                                                                                                                            ggCAGTTGACCCACCAAGATCGGAAATCACGCCCGTaaa                                                                                                      <  2:748302/39‑1 (MQ=255)

                                                                                                                            ggCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACg                                                                                            >  2:670869/1‑49 (MQ=255)

                                                                                                                            ggCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACg                                                                                            <  1:670869/49‑1 (MQ=255)

                                                                                                                            ggCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGAACGGCTCTGAATAATGCGCCCTTCGTGCTCGGTGATAGAACAGaaa  >  1:367131/1‑139 (MQ=255)

                                                                                                                            ggCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGAACGGCTCTGAATAATGCGCCCTTCGTGCTCGGTGATAGAACAGaaa  >  1:190081/1‑139 (MQ=255)

                                                                                                                             gCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGAACGGCTCTGAATAATGCGCCCTTCGTGCTc                  >  1:709286/1‑122 (MQ=255)

                                                                                                                             gCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGAACGGCTCTGAATAATGCGCCCTTCGTGCTc                  <  2:709286/122‑1 (MQ=255)

GATAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACGCACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGGCCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGAACGGCTCTGAATAATGCGCCCTTCGTGCTCGGTGATAGAACAGAAA  >  NC_000913/3159712‑3159974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A