Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I2 R1	92	50.6	2362856	94.3%	2228173	111.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,844,805	T→C	L281P (CTC→CCC)	adeD →	cryptic adenine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,844,805	0	T	C	100.0%	157.6 / NA	43	L281P (CTC→CCC)	adeD	cryptic adenine deaminase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/22); total (21/22)

AGCTGGAAGAAGGACGCCGGAAATTACAACTCGGCATGTCGTTGATGATCCGCGAAGGGTCCGCTGCCCGCAATCTCAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCTCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGATGCCTTAATTCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCG  >  NC_000913/3844679‑3844937

aGCTGGAAGAAGGACGCCGGAAATTACAACTCGGCATGTCGTTGATGATCCGCGAAGGGTCCGCTGCCCGCAATCTCAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGa                                                                                                                          >  2:1068161/1‑139 (MQ=255)

     gaagaaGGACGCCGGAAATTACAACTCGGCATGTCGTTGATGATCCGCGAAGGGTCCGCTGCCCGCAATCTCAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTa                                                                                                                     <  1:462077/139‑1 (MQ=255)

       agaagGACGCCGGAAATTACAACTCGGCATGTCGTTGATGATCCGCGAAGGGTCCGCTGCCCGCAATCTCAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAAc                                                                                                                   <  2:651906/139‑1 (MQ=255)

           ggACGCCGGAAATTACAACTCGGCATGTCGTTGATGATCCGCGAAGGGTCCGCTGCCCGCAATCTCAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGt                                                                                                               >  2:871602/1‑139 (MQ=255)

            gACGCCGGAAATTACAACTCGGCATGTCGTTGATGATCCGCGAAGGGTCCGCTGCCCGCAATCTCAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTg                                                                                                              >  1:757896/1‑139 (MQ=255)

               gCCGGAAATTACAACTCGGCATGTCGTTGATGATCCGCGAAGGGTCCGCTGCCCGCAATCTCAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGga                                                                                                           >  1:774258/1‑139 (MQ=255)

                  ggAAATTACAACTCGGCATGTCGTTGATGATCCGCGAAGGGTCCGCTGCCCGCAATCTCAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGAt                                                                                                        <  2:451051/139‑1 (MQ=255)

                      aTTACAACTCGGCATGTCGTTGATGATCCGCGAAGGGTCCGCTGCCCGCAATCTCGACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGcc                                                                                                    >  1:625016/1‑139 (MQ=255)

                          cAACTCGGCATGTCGTTGATGATCCGCGAAGGGTCCGCTGCCCGCAATCTCGACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATg                                                                                                <  2:625016/139‑1 (MQ=255)

                                  cATGTCGTTGATGATCCGCGAAGGGTCCGCTGCCCGCAATCTCAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGacac                                                                                        <  1:621511/139‑1 (MQ=255)

                                       cGTTGATGATCCGCGAAGGGTCCGCTGCCCGCAATCTCAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGgag                                                                                                          <  1:255928/116‑1 (MQ=255)

                                       cGTTGATGATCCGCGAAGGGTCCGCTGCCCGCAATCTCAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGgag                                                                                                          >  2:255928/1‑116 (MQ=255)

                                               atCCGCGAAGGGTCCGCTGCCCGCAATCTCAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAAc                                                                                                                   >  1:26471/1‑99 (MQ=255)

                                               atCCGCGAAGGGTCCGCTGCCCGCAATCTCAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAAc                                                                                                                   <  2:26471/99‑1 (MQ=255)

                                                tCCGCGAAGGGTCCGCTGCCCGCAATCTCAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGATGCCTTAAt                                                                          >  1:613700/1‑139 (MQ=255)

                                                    cgAAGGGTCCGCTGCCCGCAATCTCAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGATGCCTTAATTcgc                                                                      >  1:728780/1‑139 (MQ=255)

                                                      aaGGGTCCGCTGCCCGCAATCTCAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGATGCCTTAATTcgccg                                                                    <  2:774258/139‑1 (MQ=255)

                                                      aaGGGTCCGCTGCCCGCAATCTCAACGCGCTGGCACCGTTGATCAACGAATGTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGTTGCCTTAATTcgccg                                                                    <  1:787241/139‑1 (MQ=255)

                                                          gTCCGCTGCCCGCAATCTCAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGATGCCTTAATTCGCCGCCTg                                                                >  1:1023511/1‑139 (MQ=255)

                                                           tCCGCTGCCCGCAATCTCAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGATGCCTTAATTCGCCGCCTGa                                                               <  1:1169776/139‑1 (MQ=255)

                                                             cGCTGCCCGCAATCTCAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGATGCCTTAATTCGCCGCCTGATc                                                             <  1:1068161/139‑1 (MQ=255)

                                                               cTGCCCGCAATCTCAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGATGCCTTAATTCGCCGCCTGATCGa                                                           <  2:390095/139‑1 (MQ=255)

                                                                    cGCAATCTCAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGATGCCTTAATTCGCCGCCTGATCGaacaac                                                      >  1:784402/1‑139 (MQ=255)

                                                                            cAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGaa                                                                                              >  2:31417/1‑91 (MQ=255)

                                                                            cAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGaa                                                                                              <  1:31417/91‑1 (MQ=255)

                                                                            cAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGATGCCTTAATTCGCCGCCTGATCg                                                            >  1:910118/1‑125 (MQ=255)

                                                                            cAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGATGCCTTAATTCGCCGCCTGATCg                                                            <  2:910118/125‑1 (MQ=255)

                                                                            cAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGATGCCTTAATTCGCCGCCTGATCGAACAACACAAtgtg                                              >  2:751395/1‑139 (MQ=255)

                                                                               cgcgCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGATGCCTTAATTCGCCGCCTGATCGAACAACACAATGTGCCg                                           <  1:961691/139‑1 (MQ=255)

                                                                               cgcgCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGATGCCTTAATTCGCCGCCTGATCGAACAACACAATGTGCCg                                           <  1:220420/139‑1 (MQ=255)

                                                                                 cgcTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGATGCCTTAATTCGCCGCCTGATCGAACAACACAATGTGCCGCt                                         <  1:751395/139‑1 (MQ=255)

                                                                                    tGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGATGCCTTAATTCGCCGCCTGATCGAACAACACAATGTGCCGCTGCa                                      >  1:371629/1‑139 (MQ=255)

                                                                                                    cGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGATGCCTTAATTCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTc                      >  1:891537/1‑139 (MQ=255)

                                                                                                         ttAACAGTCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAAGACACATCg                                                                                    >  1:772027/1‑72 (MQ=255)

                                                                                                         ttAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCg                                                                                    <  2:772027/72‑1 (MQ=255)

                                                                                                           aaCAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGATGCCTTAATTCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCt               >  2:319865/1‑139 (MQ=255)

                                                                                                                cccGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGATGCCTTAATTCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCg          >  2:1476/1‑139 (MQ=255)

                                                                                                                  cGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGc                                                                                                     >  2:1136373/1‑46 (MQ=255)

                                                                                                                  cGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGc                                                                                                     <  1:1136373/46‑1 (MQ=255)

                                                                                                                  cGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGATGCCTTAATTCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGAc        >  1:143036/1‑139 (MQ=255)

                                                                                                                    cAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGATGCCTTAATTCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACgg      <  1:196289/139‑1 (MQ=255)

                                                                                                                      atgcatgcCCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGATGCCTTAATTCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGgcg    <  2:613700/139‑1 (MQ=255)

                                                                                                                        gcatgcCCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGATGCCTTAATTCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGgcgcg  <  2:379417/139‑1 (MQ=255)

AGCTGGAAGAAGGACGCCGGAAATTACAACTCGGCATGTCGTTGATGATCCGCGAAGGGTCCGCTGCCCGCAATCTCAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCTCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGATGCCTTAATTCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCG  >  NC_000913/3844679‑3844937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A