Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A13 F1 I1 R1 86 24.1 1002126 98.1% 983085 121.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,844,805 T→C L281P (CTC→CCC)  adeD → cryptic adenine deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,844,8050TC100.0% 68.3 / NA 20L281P (CTC→CCC) adeDcryptic adenine deaminase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/11);  total (9/11)

ATCAGCTGGAAGAAGGACGCCGGAAATTACAACTCGGCATGTCGTTGATGATCCGCGAAGGGTCCGCTGCCCGCAATCTCAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCTCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGATGCCTTAATTCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTC  >  NC_000913/3844676‑3844917
                                                                                                                                 |                                                                                                                
aTCAGCTGGAAGAAGGACGCCGGAAATTACAACTCGGCATGTCGTTGATGATCCGCGAAGGGTCCGCTGCCCGCAATCTCAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGa                                                                                                         <  2:188752/139‑1 (MQ=255)
      tGGAAGAAGGACGCCGGAAATTACAACTCGGCATGTCGTTGATGATCCGCGAAGGGTCCGCTGCCCGCAATCTCAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCg                                                                                                   <  1:13538/139‑1 (MQ=255)
       ggAAGAAGGACGCCGGAAATTACAACTCGGCATGTCGTTGATGATCCGCGAAGGGTCCGCTGCCCGCAATCTCAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGt                                                                                                  >  1:275890/1‑139 (MQ=255)
          agaagGACGCCGGAAATTACAACTCGGCATGTCGTTGATGATCCGCGAAGGGTCCGCTGCCCGCAATCTCAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAAc                                                                                               <  1:64493/139‑1 (MQ=255)
          agaagGACGCCGGAAATTACAACTCGGCATGTCGTTGATGATCCGCGAAGGGTCCGCTGCCCGCAATCTCAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAAc                                                                                               <  2:275620/139‑1 (MQ=255)
               gACGCCGGAAATTACAACTCGGCATGTCGTTGATGATCCGCGAAGGGTCCGCTGCCCGCAATCTCAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAAc                                                                                               >  2:62444/1‑134 (MQ=255)
               gACGCCGGAAATTACAACTCGGCATGTCGTTGATGATCCGCGAAGGGTCCGCTGCCCGCAATCTCAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAAc                                                                                               <  1:62444/134‑1 (MQ=255)
                  gCCGGAAATTACAACTCGGCATGTCGTTGATGATCCGCGAAGGGTCCGCTGCCCGCAATCTCAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGga                                                                                       <  2:207977/139‑1 (MQ=255)
                   ccGGAAATTACAACTCGGCATGTCGTTGATGATCCGCGAAGGGTCCGCTGCCCGCAATCTCAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGgag                                                                                      >  1:462640/1‑139 (MQ=255)
                             cAACTCGGCATGTCGTTGATGATCCGCGAAGGGTCCGCTGCCCGCAATCTCAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATg                                                                            <  2:450195/139‑1 (MQ=255)
                                                                             ctcAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGATGCCTTAATTCGCCGCCTGATCGAACAACACAAtg                            <  2:462640/139‑1 (MQ=255)
                                                                                               ttGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGc                                                                                 <  1:199967/68‑1 (MQ=255)
                                                                                               ttGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGc                                                                                 >  2:199967/1‑68 (MQ=255)
                                                                                               ttGATCAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGATGCCTTAATTCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCat          >  2:76838/1‑139 (MQ=255)
                                                                                                    cAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGATGCCTTAATTCGCCGCCTGATCg                                        >  2:289611/1‑104 (MQ=255)
                                                                                                    cAACGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGATGCCTTAATTCGCCGCCTGATCg                                        <  1:289611/104‑1 (MQ=255)
                                                                                                       cGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGATGCCTTAATTCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTc  >  2:109151/1‑139 (MQ=255)
                                                                                                       cGAATTTAACAGCCCGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGATGCCTTAATTCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTc  >  1:203032/1‑139 (MQ=255)
                                                                                                                     cGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGa                                                               <  1:452080/64‑1 (MQ=255)
                                                                                                                     cGCAATGCATGCCCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGa                                                               >  2:452080/1‑64 (MQ=255)
                                                                                                                                 |                                                                                                                
ATCAGCTGGAAGAAGGACGCCGGAAATTACAACTCGGCATGTCGTTGATGATCCGCGAAGGGTCCGCTGCCCGCAATCTCAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCTCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGATGCCTTAATTCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTC  >  NC_000913/3844676‑3844917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A