Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I2 R1	92	50.6	2362856	94.3%	2228173	111.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,312,566	T→C	R142R (AGA→AGG)	alsR ←	d‑allose‑inducible als operon transcriptional repressor, autorepressor, repressor of rpiR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,312,566	0	T	C	100.0%	188.5 / NA	50	R142R (AGA→AGG)	alsR	d‑allose‑inducible als operon transcriptional repressor, autorepressor, repressor of rpiR
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (26/24); total (26/24)

TGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGGTGCGTAAAGTGATGTTAAATACCTTATTCACCACATCCTGCGGCGCTTCA  >  NC_000913/4312432‑4312689

tGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTgg                                                                                                                         >  1:208904/1‑139 (MQ=255)

        cGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCt                                                                                                                      >  2:1180330/1‑134 (MQ=255)

        cGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCt                                                                                                                      <  1:1180330/134‑1 (MQ=255)

        cGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAg                                                                                                                 >  1:201854/1‑139 (MQ=255)

          aaGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGaa                                                                                                               >  2:148529/1‑139 (MQ=255)

                  atcatcatGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAgcggg                                                                                                       <  2:1086185/139‑1 (MQ=255)

                       catGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCGGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCa                                                                                                  <  1:718261/139‑1 (MQ=255)

                            tgtgAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCACGGTg                                                                                             <  2:201854/139‑1 (MQ=255)

                              tgAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGa                                                                                           >  2:652707/1‑139 (MQ=255)

                                 gCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCACgg                                                                                               <  2:450878/132‑1 (MQ=255)

                                 gCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCACgg                                                                                               >  1:450878/1‑132 (MQ=255)

                                 gCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGAtct                                                                                        >  2:1119140/1‑139 (MQ=255)

                                      aGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCtcatca                                                                                   <  1:652707/139‑1 (MQ=255)

                                               cTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACg                                                                          <  2:100278/139‑1 (MQ=255)

                                                  aCAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCtcat                                                                                     <  2:992889/125‑1 (MQ=255)

                                                  aCAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCtcat                                                                                     >  1:992889/1‑125 (MQ=255)

                                                  aCAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATc                                                                       >  2:589934/1‑139 (MQ=255)

                                                     gCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGAc                                                                    <  1:768898/139‑1 (MQ=255)

                                                     gCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGAc                                                                    <  1:967596/139‑1 (MQ=255)

                                                                tGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCAt                                                         >  1:269148/1‑139 (MQ=255)

                                                                            tgtgCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCACGGt                                                                                              <  1:739064/90‑1 (MQ=255)

                                                                            tgtgCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCACGGt                                                                                              >  2:739064/1‑90 (MQ=255)

                                                                               gCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCACGGt                                                                                              >  2:1072618/1‑87 (MQ=255)

                                                                               gCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCACGGt                                                                                              <  1:1072618/87‑1 (MQ=255)

                                                                                cTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGGTGCGTAAAGTg                                         <  2:269148/139‑1 (MQ=255)

                                                                                   tACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAAt                                                      <  1:807964/123‑1 (MQ=255)

                                                                                   tACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAAt                                                      >  2:807964/1‑123 (MQ=255)

                                                                                        cAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGGTGCGTAAAGTGATGTTaaa                                 >  2:606660/1‑139 (MQ=255)

                                                                                          gCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCa                                                                                                  <  2:698191/72‑1 (MQ=255)

                                                                                          gCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCa                                                                                                  >  1:698191/1‑72 (MQ=255)

                                                                                          gCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGGTGCGTAAAGTGATGTTAAATa                               <  1:669353/139‑1 (MQ=255)

                                                                                           cacaGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCACg                                                                                                >  1:865109/1‑73 (MQ=255)

                                                                                           cacaGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCACg                                                                                                <  2:865109/73‑1 (MQ=255)

                                                                                             caGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCa                                                                                                  <  1:1119504/69‑1 (MQ=255)

                                                                                             caGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCa                                                                                                  >  2:1119504/1‑69 (MQ=255)

                                                                                             caGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGGTGCGTAAAGTGATGTTAAATACCt                            >  1:1061662/1‑139 (MQ=255)

                                                                                                aTAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGGTGCg                                                >  2:1027103/1‑116 (MQ=255)

                                                                                                aTAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGGTGCg                                                <  1:1027103/116‑1 (MQ=255)

                                                                                                aTAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGGTGCGTAAAGTGATGTTAAATACCTTAt                         <  1:148529/139‑1 (MQ=255)

                                                                                                aTAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGGTGCGTAAAGTGATGTTAAATACCTTAt                         >  1:785413/1‑139 (MQ=255)

                                                                                                aTAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGGTGCGTAAAGTGATGTTAAATACCTTAt                         >  2:1106840/1‑139 (MQ=255)

                                                                                                aTAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGGTGCGTAAAGTGATGTTAAATACCTTAt                         <  2:674323/139‑1 (MQ=255)

                                                                                                 tAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGGTGCGTAAAGTGATGTTAAATACCTTAtt                        <  1:1106840/139‑1 (MQ=255)

                                                                                                     aTTTGATCCTCCGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGGTGCGTAAAGTGATGTTAAATACCTTATTcacc                    >  1:572392/1‑139 (MQ=255)

                                                                                                              tccGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCtcatca                                                                                   >  2:7224/1‑67 (MQ=255)

                                                                                                              tccGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCtcatca                                                                                   <  1:7224/67‑1 (MQ=255)

                                                                                                              tccGGCACCGTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGGTGCGTAAAGTGATGTTAAATACCTTATTCACCACATCCTgc           >  1:1040775/1‑139 (MQ=255)

                                                                                                                       gTACAAATCCCGCTGCCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGGTGCGTAAAGTGATGTTAAATACCTTATTCACCACATCCTGCGGCGCTtca  >  1:634294/1‑139 (MQ=255)

                                                                                                                                  gCTGCCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGAcc                                                               >  1:37351/1‑67 (MQ=255)

                                                                                                                                  gCTGCCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGAcc                                                               <  2:37351/67‑1 (MQ=255)

TGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGGTGCGTAAAGTGATGTTAAATACCTTATTCACCACATCCTGCGGCGCTTCA  >  NC_000913/4312432‑4312689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A