Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A13 F1 I2 R1 92 50.6 2362856 94.3% 2228173 111.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,431,613 T→C E124G (GAA→GGA)  ytfE ← iron‑sulfur cluster repair protein RIC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,431,6130TC100.0% 211.6 / NA 55E124G (GAA→GGA) ytfEiron‑sulfur cluster repair protein RIC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/27);  total (28/27)

CTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTTCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCA  >  NC_000913/4431479‑4431742
                                                                                                                                      |                                                                                                                                 
cTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATg                                                                                                                               >  2:811974/1‑139 (MQ=255)
     agcagTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATgcagca                                                                                                                          <  2:963975/139‑1 (MQ=255)
           tCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGt                                                                                                                      >  2:413995/1‑137 (MQ=255)
           tCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGt                                                                                                                      <  1:413995/137‑1 (MQ=255)
           tCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCa                                                                                                                    <  2:908826/139‑1 (MQ=255)
            cGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAg                                                                                                                   <  2:424461/139‑1 (MQ=255)
                                cTTTCCATTACGCTGATTGGCCCCATTGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTg                                                                                               >  2:1133610/1‑139 (MQ=255)
                                         aCGCTGATTGGCCCCATTGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCg                                                                                      >  1:1034035/1‑139 (MQ=255)
                                             tGATTGGCCCCATTGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGttt                                                                                  >  2:1098724/1‑139 (MQ=255)
                                                        aTTGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGaa                                                                       <  1:1133610/139‑1 (MQ=255)
                                                         ttGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATgca                                                                                                                             >  2:635849/1‑84 (MQ=255)
                                                         ttGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATgca                                                                                                                             <  1:635849/84‑1 (MQ=255)
                                                         ttGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTc                                                                                                         <  2:1034551/104‑1 (MQ=255)
                                                         ttGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGAGCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTc                                                                                                         >  1:1034551/1‑104 (MQ=255)
                                                             cTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACgcgc                                                                  <  1:127000/139‑1 (MQ=255)
                                                             cTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACgcgc                                                                  <  2:363617/139‑1 (MQ=255)
                                                                        tGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGt                                                       <  1:577083/139‑1 (MQ=255)
                                                                          ccTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCg                                                     <  2:815396/139‑1 (MQ=255)
                                                                              gTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATg                                                                                                                               >  1:199890/1‑61 (MQ=255)
                                                                              gTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATg                                                                                                                               <  2:199890/61‑1 (MQ=255)
                                                                              gTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTgg                                                                                              >  1:1157912/1‑94 (MQ=255)
                                                                              gTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTgg                                                                                              <  2:1157912/94‑1 (MQ=255)
                                                                              gTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCt                                                                                        >  1:257470/1‑100 (MQ=255)
                                                                              gTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCt                                                                                        <  2:257470/100‑1 (MQ=255)
                                                                              gTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTg                                                 >  2:805005/1‑139 (MQ=255)
                                                                              gTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTg                                                 >  2:319704/1‑139 (MQ=255)
                                                                                      atcGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCa                                         <  2:1034035/139‑1 (MQ=255)
                                                                                        cGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATgcagca                                                                                                                          <  2:1113299/56‑1 (MQ=255)
                                                                                        cGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATgcagca                                                                                                                          >  1:1113299/1‑56 (MQ=255)
                                                                                        cGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCGGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGc                                       <  1:805005/139‑1 (MQ=255)
                                                                                         gggAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTgg                                                                                              <  1:1082716/83‑1 (MQ=255)
                                                                                         gggAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTgg                                                                                              >  2:1082716/1‑83 (MQ=255)
                                                                                                  aTCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGtt                             >  2:1078924/1‑139 (MQ=255)
                                                                                                      gCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAg                                                                                                       >  1:176588/1‑61 (MQ=255)
                                                                                                      gCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAg                                                                                                       <  2:176588/61‑1 (MQ=255)
                                                                                                         cttcttTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGt                                                       >  2:1159636/1‑106 (MQ=255)
                                                                                                         cttcttTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGt                                                       <  1:1159636/106‑1 (MQ=255)
                                                                                                         cttcttTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTcgcg                      >  2:256217/1‑139 (MQ=255)
                                                                                                          ttcttTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCgg                     >  1:272865/1‑139 (MQ=255)
                                                                                                            cttTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTg                   <  2:272865/139‑1 (MQ=255)
                                                                                                            cttTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTg                   <  1:811974/139‑1 (MQ=255)
                                                                                                                 atcatGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGAt              <  1:256217/139‑1 (MQ=255)
                                                                                                                      gtgGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCt                                                                                        >  1:773658/1‑60 (MQ=255)
                                                                                                                      gtgGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCt                                                                                        <  2:773658/60‑1 (MQ=255)
                                                                                                                      gtgGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTg                   >  2:559269/1‑129 (MQ=255)
                                                                                                                      gtgGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTg                   <  1:559269/129‑1 (MQ=255)
                                                                                                                      gtgGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTgg         >  2:416655/1‑139 (MQ=255)
                                                                                                                      gtgGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTgg         >  1:464700/1‑139 (MQ=255)
                                                                                                                      gtgGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTgg         >  2:1102827/1‑139 (MQ=255)
                                                                                                                       tgGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGt        >  2:906537/1‑139 (MQ=255)
                                                                                                                         gCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGAc                 >  1:1168251/1‑128 (MQ=255)
                                                                                                                         gCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGAc                 <  2:1168251/128‑1 (MQ=255)
                                                                                                                         gCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAg      >  2:764541/1‑139 (MQ=255)
                                                                                                                           tGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAgcg    >  1:517369/1‑139 (MQ=255)
                                                                                                                             gAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCa  <  1:1102827/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                 
CTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTTCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCA  >  NC_000913/4431479‑4431742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A