Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F1 I1 R1
|
86 |
24.1 |
1002126 |
98.1% |
983085 |
121.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
671,531 |
A→G |
G25G (GGT→GGC) |
holA ← |
DNA polymerase III, delta subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 671,531 | 0 | A | G | 100.0%
| 45.7
/ NA
| 13 | G25G (GGT→GGC) | holA | DNA polymerase III, delta subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (9/4); total (9/4) |
ATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGT > NC_000913/671415‑671661
|
aTCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTGCCAAGTAAAAGATACGCcgcgc > 2:452481/1‑139 (MQ=255)
ttCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTGCCAAGTAAAAGATACGCCGCGCGCAGcc < 2:433847/139‑1 (MQ=255)
aaTGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTGCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCgg > 2:4535/1‑139 (MQ=255)
gtggtgTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTGCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCggg < 1:244538/139‑1 (MQ=255)
tggtgTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTGCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGt > 1:2636/1‑139 (MQ=255)
tcGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTGCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGaa < 1:452481/139‑1 (MQ=255)
gAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTGCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAatc < 2:120520/139‑1 (MQ=255)
cAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTGCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGc > 2:168850/1‑139 (MQ=255)
gCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTGCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGc > 2:229856/1‑139 (MQ=255)
tCCTGGCTTTCCTGCAATAACAGAGGATCGTTGCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAgg > 1:294597/1‑139 (MQ=255)
cTTTCCTGCAATAACAGAGGATCGTTGCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGc > 2:289359/1‑139 (MQ=255)
agagGATCGTTGCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTtgt > 2:202607/1‑139 (MQ=255)
ggATCGTTGCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGt > 1:419128/1‑139 (MQ=255)
|
ATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGT > NC_000913/671415‑671661
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
