Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A9 F1 I1 R1
|
64 |
69.7 |
3300380 |
93.4% |
3082554 |
111.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
678,376 |
C→T |
P321L (CCG→CTG) |
djlB → |
putative HscC co‑chaperone, uncharacterized J domain‑containing protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 678,376 | 0 | C | T | 89.3%
| 149.5
/ 9.9
| 56 | P321L (CCG→CTG) | djlB | putative HscC co‑chaperone, uncharacterized J domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base C (3/3); new base T (28/22); total (31/25) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-01 |
ACTACTGGCGCGAGTATCCCTCCACCCAGGCGGATGCTTTAATGTTGCAATGGTGCCGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACCGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAACTCTCTGCGCTGGGGCGTTCACTGGTCGAGATGGTTTTGCACAAAGGACGTAAGCCGCG > NC_000913/678241‑678505
|
actactGGCGCGAGTATCCCTCCACCCAGGCGGATGCTTTAATGTTGCAATGGTGCCGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCt < 2:690497/139‑1 (MQ=255)
aGTATCCCTCCACCCAGGCGGATGCTTTAATGTTGCAATGGTGCCGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTccc > 2:930648/1‑139 (MQ=255)
tATCCCTCCACCCAGGCGGATGCTTTAATGTTGCAATGGTGCCGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCg > 1:589910/1‑139 (MQ=255)
tATCCCTCCACCCAGGCGGATGCTTTAATGTTGCAATGGTGCCGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACCGCTACTTTATGTCCCCg > 1:365635/1‑139 (MQ=255)
tCCACCCAGGCGGATGCTTTAATGTTGCAATGGTGCCGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACa < 1:1498999/139‑1 (MQ=255)
aCCCAGGCGGATGCTTTAATGTTGCAATGGTGCCGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAgcg > 2:337733/1‑139 (MQ=255)
cccAGGCGGATGCTTTAATGTTGCAATGGTGCCGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAgcgc > 1:514016/1‑139 (MQ=255)
ttGCAATGGTGCCGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTtat < 2:589910/139‑1 (MQ=255)
ttGCAATGGTGCCGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACCGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTtat < 2:365635/139‑1 (MQ=255)
gCAATGGTGCCGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCAttt < 2:885262/135‑1 (MQ=255)
gCAATGGTGCCGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCAttt > 1:885262/1‑135 (MQ=255)
cAATGGTGCCGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACg > 1:5047/1‑139 (MQ=255)
gTGCCGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACg < 2:810950/134‑1 (MQ=255)
gTGCCGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACg > 1:810950/1‑134 (MQ=255)
gTGCCATCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATa > 1:1495390/1‑139 (MQ=255)
gCCGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACCGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACt < 2:160590/139‑1 (MQ=255)
ccGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTc > 2:303007/1‑139 (MQ=255)
ccGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACCGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTc > 2:426170/1‑139 (MQ=255)
cAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCg < 1:950443/139‑1 (MQ=255)
cGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTtat < 1:51896/118‑1 (MQ=255)
cGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTtat > 2:51896/1‑118 (MQ=255)
gATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAActct < 1:328073/139‑1 (MQ=255)
gATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAActct < 2:420/139‑1 (MQ=255)
ttattaCCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAActctct < 2:1489191/139‑1 (MQ=255)
cATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTtat > 2:333263/1‑102 (MQ=255)
cATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTtat < 1:333263/102‑1 (MQ=255)
aTTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAACTCTCTGCGCTgggg > 2:1466669/1‑139 (MQ=255)
ttACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAACTCTCTGCGCTGGGGc > 1:694795/1‑139 (MQ=255)
tACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAACTCTCTGCGCTGGGGCg > 2:1271525/1‑139 (MQ=255)
gTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAACTCTCTGCGCTGGGGCGTTCAc > 2:1148019/1‑139 (MQ=255)
tgatgatgaTTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTtat > 1:1484581/1‑93 (MQ=255)
tgatgatgaTTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTtat < 2:1484581/93‑1 (MQ=255)
atgatgatTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTa > 1:1319850/1‑76 (MQ=255)
atgatgatTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTa < 2:1319850/76‑1 (MQ=255)
gatgatTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAACTCTCTGCGCTGGGGCGTTCACTGGTc > 2:1570062/1‑139 (MQ=255)
atTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAACTCTCTGCGCTGGGGCGTTCACTGGTCgaga < 2:1495390/139‑1 (MQ=255)
aaGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAACTCTCTGCGCTGGGGCGTTCACTGGTCGAGATGGt > 1:236289/1‑139 (MQ=255)
cgTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAACTCTCTGCGCTGGGGCGTTCACTGGTCGAGATGGTTTTGc > 2:1457009/1‑139 (MQ=255)
ggTTAACGATCAGGGAAAACCGCTACTTTATGTCCCCg < 2:126631/38‑1 (MQ=255)
ggTTAACGATCAGGGAAAACCGCTACTTTATGTCCCCg > 1:126631/1‑38 (MQ=255)
ttAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGtt > 2:72923/1‑57 (MQ=255)
ttAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGtt < 1:72923/57‑1 (MQ=255)
ttAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAACTCTCTGCGCTGGGGCGTTCACTGGTCGAGATGGTTTTGCACAAAGGACg < 1:261563/139‑1 (MQ=255)
tAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAACTCTCTGCGCTGGGGCGTTCACTGGTCGAGATGGTTTTGCACAAAGGACGt > 1:179573/1‑139 (MQ=255)
cGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGa < 2:1574235/36‑1 (MQ=255)
cGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGa > 1:1574235/1‑36 (MQ=255)
tCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAACTCTCTGCGCTGGGGCGTTCACTg > 1:684152/1‑106 (MQ=255)
tCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAACTCTCTGCGCTGGGGCGTTCACTg < 2:684152/106‑1 (MQ=255)
tCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAACTCTCTGCGCTGGGGCGTTCACTGGTCGAGATGGTTTTGCACAAAGGACGTAAGCcg < 1:303007/139‑1 (MQ=255)
aGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTAcg > 1:876836/1‑44 (MQ=255)
aGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTAcg < 2:876836/44‑1 (MQ=255)
aGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCAttt > 1:1348914/1‑55 (MQ=255)
aGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCAttt < 2:1348914/55‑1 (MQ=255)
aGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTta < 1:765644/56‑1 (MQ=255)
aGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTta > 2:765644/1‑56 (MQ=255)
aGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAACTCTCTGAGCTGGGGCGTTCACTGGTCGAGATGGTTTTGCACAAAGGACGTAAGCcgcg > 2:929186/1‑139 (MQ=255)
|
ACTACTGGCGCGAGTATCCCTCCACCCAGGCGGATGCTTTAATGTTGCAATGGTGCCGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACCGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAACTCTCTGCGCTGGGGCGTTCACTGGTCGAGATGGTTTTGCACAAAGGACGTAAGCCGCG > NC_000913/678241‑678505
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
