Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F1 I1 R1
|
86 |
24.1 |
1002126 |
98.1% |
983085 |
121.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,995,769 |
T→C |
intergenic (‑410/‑49) |
uvrY ← / → yecF |
response regulator in two‑component regulatory system with BarA/DUF2594 family protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,995,769 | 0 | T | C | 100.0%
| 66.5
/ NA
| 19 | intergenic (‑410/‑49) | uvrY/yecF | response regulator in two‑component regulatory system with BarA/DUF2594 family protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (13/6); total (13/6) |
ACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCTTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGAT > NC_000913/1995686‑1995903
|
aCGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTa > 1:431020/1‑139 (MQ=255)
tATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGc > 1:59875/1‑139 (MQ=255)
cTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTa < 2:467892/124‑1 (MQ=255)
cTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTa > 1:467892/1‑124 (MQ=255)
cTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTa < 2:59875/139‑1 (MQ=255)
cTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTa > 2:401594/1‑139 (MQ=255)
ccGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTa < 2:280962/136‑1 (MQ=255)
ccGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTa > 1:280962/1‑136 (MQ=255)
cGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGc < 1:368841/139‑1 (MQ=255)
gCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGaataa > 1:298975/1‑139 (MQ=255)
ataCTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCt < 1:401594/139‑1 (MQ=255)
aCTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAAAAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGa > 2:215458/1‑139 (MQ=255)
cgccgcCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAGTGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCt > 2:21221/1‑139 (MQ=255)
cgccgcCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCt > 2:384174/1‑139 (MQ=255)
cgccgcCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGAAGCt > 1:311461/1‑139 (MQ=255)
ccgccCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGtctc > 2:189096/1‑113 (MQ=255)
ccgccCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGtctc < 1:189096/113‑1 (MQ=255)
ccgccCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGa > 1:165284/1‑139 (MQ=255)
cgccCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGAt > 1:403909/1‑139 (MQ=255)
|
ACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCTTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGAT > NC_000913/1995686‑1995903
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
