Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I1 R1	86	24.1	1002126	98.1%	983085	121.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,233,485	(T)_6→7	coding (608/720 nt)	sanA →	DUF218 superfamily vancomycin high temperature exclusion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,233,479	1	.	T	100.0%	90.0 / NA	27	R201L (CGT→CTT)	sanA	DUF218 superfamily vancomycin high temperature exclusion protein
Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (14/13); total (14/13)

TGCGCTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCG‑TTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACA  >  NC_000913/2233345‑2233610

tGCGCTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCG‑tttt                                                                                                                                 >  1:19494/1‑139 (MQ=255)

  cgcTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCG‑tttttt                                                                                                                               >  1:163981/1‑139 (MQ=255)

   gcTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCG‑ttttttt                                                                                                                              >  1:45933/1‑138 (MQ=255)

        atatGGGGATTCAGGCTCAGTGTTATGCCGTACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTTAGGGc                                                                                                                          <  1:419670/139‑1 (MQ=255)

                       cTCAGTGTTATGCCGTACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTc                                                                                                           <  2:156585/139‑1 (MQ=255)

                        tCAGTGTTATGCCGTACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTcc                                                                                                          >  2:109053/1‑139 (MQ=255)

                            tgtTATGCCGTACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCt                                                                                                      >  1:104338/1‑139 (MQ=255)

                                aTGCCGTACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGc                                                                                                  <  2:45933/139‑1 (MQ=255)

                                      tACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAgg                                                                                            >  2:439580/1‑139 (MQ=255)

                                      tACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATA‑TTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTc                                                                                                                  >  1:16604/1‑116 (MQ=255)

                                      tACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATA‑TTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTc                                                                                                                  <  2:16604/116‑1 (MQ=255)

                                               cGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAg                                                                                   <  2:19494/139‑1 (MQ=255)

                                                               aGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATcc                                                                   <  2:294175/139‑1 (MQ=255)

                                                                    gTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCAcc                                                                                               <  1:297540/106‑1 (MQ=255)

                                                                    gTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCAcc                                                                                               >  2:297540/1‑106 (MQ=255)

                                                                           tGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAg                                                                                             <  2:102378/101‑1 (MQ=255)

                                                                           tGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAg                                                                                             >  1:102378/1‑101 (MQ=255)

                                                                               tttGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGaa                                                   >  2:166854/1‑139 (MQ=255)

                                                                                  gccgccCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTGCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAg                                                >  1:302647/1‑139 (MQ=255)

                                                                                                  cgcTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCTGCCGTCACACCCGAACAGTTACTTGAATTACaaa                                <  2:104338/139‑1 (MQ=255)

                                                                                                               ttATATTTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGaaa                   <  1:416661/139‑1 (MQ=255)

                                                                                                                 atatTTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGaaa                   <  1:450479/137‑1 (MQ=255)

                                                                                                                 atatTTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGaaa                   >  2:450479/1‑137 (MQ=255)

                                                                                                                 atatTTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAagt                 >  2:481439/1‑139 (MQ=255)

                                                                                                                    tttttAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAagtagt              <  2:163981/139‑1 (MQ=255)

                                                                                                                       ttAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTAt           <  2:254465/139‑1 (MQ=255)

                                                                                                                       ttAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTAt           >  1:8078/1‑139 (MQ=255)

                                                                                                                       ttAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTAt           >  2:461081/1‑139 (MQ=255)

                                                                                                                       ttAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAAAAACAAGGAAAGTAGTTAt           >  2:255317/1‑139 (MQ=255)

                                                                                                                                aaCCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTAca  >  1:407956/1‑139 (MQ=255)

TGCGCTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCG‑TTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACA  >  NC_000913/2233345‑2233610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A