Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I1 R1	86	24.1	1002126	98.1%	983085	121.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,340,022	C→T	G152G (GGC→GGT)	ubiG →	bifunctional 3‑demethylubiquinone‑9 3‑methyltransferase/ 2‑octaprenyl‑6‑hydroxy phenol methylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,340,022	0	C	T	100.0%	107.2 / NA	31	G152G (GGC→GGT)	ubiG	bifunctional 3‑demethylubiquinone‑9 3‑methyltransferase/ 2‑octaprenyl‑6‑hydroxy phenol methylase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (16/15); total (16/15)

GTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCATGGTGCCCAAAGGCACGCATGATGTGAAGAAGTTTATTAAACCGGCAG  >  NC_000913/2339888‑2340152

gTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGgtggcg                                                                                                                                <  2:493819/139‑1 (MQ=255)

     ggAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGTGGCGATGTc                                                                                                                           <  2:204194/139‑1 (MQ=255)

          ccGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGTGGCGATGTCttttt                                                                                                                      >  1:232924/1‑139 (MQ=255)

                     cACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGTGGCGATGTCTTTTTCTCGACACTTa                                                                                                           >  2:123194/1‑139 (MQ=255)

                             aaaaCATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGTGCAACTGGTGAAACCAGGTGGCGATGTCTTTTTCTCGACACTTAACCGCAAc                                                                                                   >  2:91469/1‑139 (MQ=255)

                                                     ggtgACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGTGGTGATGTCTTTTACTCGACACTTAACCGCAACGGCAAGTCATGGCTGAt                                                                                  <  2:318022/132‑1 (MQ=255)

                                                     ggtgACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGTGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGAt                                                                                  >  1:318022/1‑132 (MQ=255)

                                                       tgACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGTGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCggtggt                                                                         >  1:231826/1‑139 (MQ=255)

                                                         aCCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGTGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGtggttg                                                                       >  2:426260/1‑139 (MQ=255)

                                                         aCCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGTGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGtggttg                                                                       >  1:274355/1‑139 (MQ=255)

                                                           cTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGTGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGtggttggt                                                                     >  1:302332/1‑139 (MQ=255)

                                                               aTGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCTGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGTGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCgg                                                                 <  2:302332/139‑1 (MQ=255)

                                                                        cTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGTGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATAttt                                                        >  2:264097/1‑139 (MQ=255)

                                                                              cACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGTGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCa                                                  <  2:184770/139‑1 (MQ=255)

                                                                                cGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGTGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCATg                                                <  1:123194/139‑1 (MQ=255)

                                                                                  tCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGTGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCATGGt                                              >  2:106123/1‑139 (MQ=255)

                                                                                       gATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGTGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCATGGTGCCCa                                         <  2:274355/139‑1 (MQ=255)

                                                                                           cGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGGAACCAGGTGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCATGGTGCCCAAAgg                                     <  1:412932/139‑1 (MQ=255)

                                                                                            gCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGTGGCGATGTCTTTTTCTCGACACTTAACCGCAACgg                                                                                                 <  2:466378/78‑1 (MQ=255)

                                                                                            gCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGTGGCGATGTCTTTTTCTCGACACTTAACCGCAACgg                                                                                                 >  1:466378/1‑78 (MQ=255)

                                                                                             cagtcagtGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGTGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCa                                                  >  2:330209/1‑124 (MQ=255)

                                                                                             cagtcagtGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGTGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCa                                                  <  1:330209/124‑1 (MQ=255)

                                                                                              agtcagtGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGTGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCATGGTGCCCAAAGGCAc                                  <  2:11130/139‑1 (MQ=255)

                                                                                                tcagtGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGTGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCATGGTGCCCAAAGGCATGc                                <  2:240043/139‑1 (MQ=255)

                                                                                                 cagtGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGTGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCATGGTGCCCAAAGGCACGCa                               <  1:106123/139‑1 (MQ=255)

                                                                                                   gtGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGTGGCGCTGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCATGGTGCCCAAAGGCACGCatg                             >  1:84101/1‑139 (MQ=255)

                                                                                                                 tgCGCAACTGGTGAAACCAGGTGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCATGGTGCCCAAAGGCACGCa                               >  1:384362/1‑123 (MQ=255)

                                                                                                                 tgCGCAACTGGTGAAACCAGGTGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCATGGTGCCCAAAGGCACGCa                               <  2:384362/123‑1 (MQ=255)

                                                                                                                 tgCGCAACTAGTGAAACCAGGTGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCATGGTGCCCAAAGGCACGCATGATGTGAAGAAGttt               >  1:8224/1‑139 (MQ=255)

                                                                                                                     cAACTGGTGAAACCAGGTGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCATGGTGccc                                          >  2:152324/1‑108 (MQ=255)

                                                                                                                     cAACTGGTGAAACCAGGTGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCATGGTGccc                                          <  1:152324/108‑1 (MQ=255)

                                                                                                                              aaaCCAGGTGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCATGGTGCCCAAAGGCACGCATGATGTGAAGAAGTTTATTAAACCGGCAg  <  1:264097/139‑1 (MQ=255)

GTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCATGGTGCCCAAAGGCACGCATGATGTGAAGAAGTTTATTAAACCGGCAG  >  NC_000913/2339888‑2340152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A