Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I1 R1	86	24.1	1002126	98.1%	983085	121.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,226,690	A→G	T153A (ACC→GCC)	ygjI →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,226,690	0	A	G	100.0%	149.8 / NA	41	T153A (ACC→GCC)	ygjI	putative transporter
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (23/18); total (23/18)

GCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCTGACGCCATCACCGTTGACGCGATGATCCCGAACTTCAACT  >  NC_000913/3226564‑3226819

gCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACtt                                                                                                                       >  2:269289/1‑139 (MQ=255)

           cTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGAtg                                                                                                            <  2:411693/139‑1 (MQ=255)

              ccTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGAtgctg                                                                                                         >  1:326813/1‑139 (MQ=255)

              ccTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAAGGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGAtgctg                                                                                                         >  1:485834/1‑139 (MQ=255)

                        gAATATATTATGACGCCTGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGc                                                                                               >  2:197014/1‑139 (MQ=255)

                            atatTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCtctc                                                                                           <  2:275678/139‑1 (MQ=255)

                             tattatGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAAc                                                                                                                   >  2:42963/1‑114 (MQ=255)

                             tattatGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAAc                                                                                                                   <  1:42963/114‑1 (MQ=255)

                                    aCGGCGGTTGCCACCACCATAATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACAttt                                                                                   >  2:340063/1‑139 (MQ=255)

                                    aCGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACAttt                                                                                   >  2:415282/1‑139 (MQ=255)

                                       ccGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTAc                                                                                >  2:295740/1‑139 (MQ=255)

                                       ccGGTTGCCACCACCATTATCAGTAAGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTAc                                                                                >  2:463414/1‑139 (MQ=255)

                                                   accaTTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACgg                                                                    <  2:326813/139‑1 (MQ=255)

                                                    ccaTTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGgc                                                                   >  1:437778/1‑139 (MQ=255)

                                                       ttATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCt                                                                >  1:438111/1‑139 (MQ=255)

                                                              tATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGACACTGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTggc                                                         >  1:480176/1‑139 (MQ=255)

                                                              tATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTggc                                                         >  1:10120/1‑139 (MQ=255)

                                                                  gTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAGTGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTTgcggcg                                                     >  1:19322/1‑139 (MQ=255)

                                                                    gCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTa                                                   <  1:340063/139‑1 (MQ=255)

                                                                    gCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTa                                                   <  2:294539/139‑1 (MQ=255)

                                                                     cTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGAtgc                                                                                                           <  2:148088/82‑1 (MQ=255)

                                                                     cTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGAtgc                                                                                                           >  1:148088/1‑82 (MQ=255)

                                                                     cTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTAc                                                  >  2:353535/1‑139 (MQ=255)

                                                                      tGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACa                                                 >  2:190368/1‑139 (MQ=255)

                                                                        ttCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGcc                                                                                                                                 <  1:134650/57‑1 (MQ=255)

                                                                        ttCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGcc                                                                                                                                 >  2:134650/1‑57 (MQ=255)

                                                                        ttCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGc                                               >  2:267133/1‑139 (MQ=255)

                                                                        ttCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGc                                               <  2:10120/139‑1 (MQ=255)

                                                                        ttCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGc                                               <  1:415282/139‑1 (MQ=255)

                                                                          cGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGct                                                                                              <  2:167360/90‑1 (MQ=255)

                                                                          cGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGct                                                                                              >  1:167360/1‑90 (MQ=255)

                                                                           gCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCctg                                            <  1:463414/139‑1 (MQ=255)

                                                                             cTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCctgct                                          <  1:190368/139‑1 (MQ=255)

                                                                                     ccTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCTGa                                        <  1:462722/133‑1 (MQ=255)

                                                                                     ccTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCTGa                                        >  2:462722/1‑133 (MQ=255)

                                                                                       tGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCTGACGCCATCa                                <  2:36657/139‑1 (MQ=255)

                                                                                                       gggCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCTGACGCCATCACCGTTGAcg                       >  1:85111/1‑132 (MQ=255)

                                                                                                       gggCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCTGACGCCATCACCGTTGAcg                       <  2:85111/132‑1 (MQ=255)

                                                                                                         gCGACAATGTTGGGGTCAATTGCCTACGTCACTTAGACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCTGACGCCATCACCGTTGACGCGATGATcc              <  1:492032/139‑1 (MQ=255)

                                                                                                         gCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCTGACGCCATCACCGTTGACGCGATGATcc              >  2:495176/1‑139 (MQ=255)

                                                                                                                     gggCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCTGACGCCATCACCGTTGACGCGATGATCCCGAACTTCAACt  <  1:349148/139‑1 (MQ=255)

GCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCTGACGCCATCACCGTTGACGCGATGATCCCGAACTTCAACT  >  NC_000913/3226564‑3226819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A