Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A10 F1 I2 R1
|
67 |
36.0 |
1614110 |
95.1% |
1535018 |
114.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,621,054 |
A→G |
H28H (CAT→CAC) |
eamA ← |
cysteine and O‑acetyl‑L‑serine efflux system |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,621,054 | 0 | A | G | 100.0%
| 61.6
/ NA
| 17 | H28H (CAT→CAC) | eamA | cysteine and O‑acetyl‑L‑serine efflux system |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (9/8); total (9/8) |
TGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAA > NC_000913/1620923‑1621185
|
tGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTGTGAAGcc < 1:419261/139‑1 (MQ=255)
aCCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTGTGAAGCCCCACTTTGATGACCACAAAATTTAg > 1:49218/1‑139 (MQ=255)
caAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTGTGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACg < 2:49218/139‑1 (MQ=255)
aTTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTGTGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGAcc > 1:67311/1‑139 (MQ=255)
gTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTGTGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCaa < 2:67311/139‑1 (MQ=255)
cGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTGTGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACAcccc > 1:699929/1‑139 (MQ=255)
aGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTGTGAAGcc > 1:120065/1‑65 (MQ=255)
aGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTGTGAAGcc < 2:120065/65‑1 (MQ=255)
ggAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTGTGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTc > 2:231121/1‑139 (MQ=255)
cGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTGTGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGcc > 2:88820/1‑139 (MQ=255)
aCCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTGTGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCctc < 1:458832/139‑1 (MQ=255)
aGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTGTGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGa > 1:370335/1‑139 (MQ=255)
aGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTGTGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCAt < 2:699929/139‑1 (MQ=255)
aaaCCGGCCAGCATCAGCGGTGGCATGTTGTGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCctct > 1:499060/1‑125 (MQ=255)
aaaCCGGCCAGCATCAGCGGTGGCATGTTGTGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCctct < 2:499060/125‑1 (MQ=255)
aaaCCGGCCAGCATCAGCGGTGGCATGTTGTGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCtttt > 1:633595/1‑139 (MQ=255)
cgttcgCATGTTGTCAAGCCCCACTTTGATGACCACAAAATTCAGCCCCCATACCACCACTACCATTAGCGCCAACCCCCCATCTTTCCCCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAg < 2:230049/134‑1 (MQ=255)
gCATGTTGTGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGaa < 2:417646/139‑1 (MQ=255)
|
TGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAA > NC_000913/1620923‑1621185
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
