Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I2 R1	67	36.0	1614110	95.1%	1535018	114.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,319,725	T→C	E51G (GAA→GGA)	rcsC ←	hybrid sensory kinase in two‑component regulatory system with RcsB and YojN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,319,725	0	T	C	100.0%	156.8 / NA	42	E51G (GAA→GGA)	rcsC	hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/21); total (21/21)

TTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAG  >  NC_000913/2319594‑2319860

tttCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACg                                                                                                                                  <  2:602241/139‑1 (MQ=255)

         cACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGa                                                                                                                         >  1:701075/1‑139 (MQ=255)

           cgcgGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGAtt                                                                                                                       <  1:160071/139‑1 (MQ=255)

           cgcgGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGAtt                                                                                                                       <  1:731522/139‑1 (MQ=255)

           cgcgGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGAtt                                                                                                                       <  1:30028/139‑1 (MQ=255)

              ggggAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATtctc                                                                                                                    <  1:436868/139‑1 (MQ=255)

                      cacaCCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGt                                                                                                            <  1:157335/139‑1 (MQ=255)

                          ccGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACg                                                                                                        >  2:431532/1‑139 (MQ=255)

                            gTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCg                                                                                                                   >  2:614328/1‑126 (MQ=255)

                            gTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCg                                                                                                                   <  1:614328/126‑1 (MQ=255)

                                tCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTaa                                                                                                  >  2:12186/1‑139 (MQ=255)

                                       aTAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTa                                                                                           >  1:766726/1‑139 (MQ=255)

                                                 ttCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGAt                                                                                 >  2:439052/1‑139 (MQ=255)

                                                     gCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGaaa                                                                             <  1:431532/139‑1 (MQ=255)

                                                                     ctTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCa                                                             <  1:366423/139‑1 (MQ=255)

                                                                      tttCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCag                                                            <  2:390855/139‑1 (MQ=255)

                                                                       ttCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCaga                                                           >  1:762802/1‑139 (MQ=255)

                                                                               atcaGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAAc                                                   >  2:710678/1‑139 (MQ=255)

                                                                                   gagGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCa                                               <  2:701075/139‑1 (MQ=255)

                                                                                      gTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCGCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTGACGCCAATg                                            >  2:548888/1‑139 (MQ=255)

                                                                                       tGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGc                                           <  2:231780/139‑1 (MQ=255)

                                                                                         cGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGctc                                         >  2:513271/1‑139 (MQ=255)

                                                                                         cGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGctc                                         >  2:462560/1‑139 (MQ=255)

                                                                                         cGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGctc                                         >  2:434183/1‑139 (MQ=255)

                                                                                         cGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGctc                                         <  1:439052/139‑1 (MQ=255)

                                                                                           ttGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACg                                                                                                >  2:745740/1‑82 (MQ=255)

                                                                                           ttGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACg                                                                                                <  1:745740/82‑1 (MQ=255)

                                                                                           ttGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTg                                       >  1:231486/1‑139 (MQ=255)

                                                                                           ttGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTg                                       <  1:462560/139‑1 (MQ=255)

                                                                                                   aGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGc                                           >  2:297844/1‑127 (MQ=255)

                                                                                                   aGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGc                                           <  1:297844/127‑1 (MQ=255)

                                                                                                        tGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAgc                             >  2:69611/1‑136 (MQ=255)

                                                                                                        tGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAgc                             <  1:69611/136‑1 (MQ=255)

                                                                                                        tGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAGcgcg                          >  1:801902/1‑139 (MQ=255)

                                                                                                          aGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAGCGCGAg                        >  1:232604/1‑139 (MQ=255)

                                                                                                          aGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAGCGCGAg                        <  2:766726/139‑1 (MQ=255)

                                                                                                               gATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATg                                            >  2:199598/1‑114 (MQ=255)

                                                                                                               gATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATg                                            <  1:199598/114‑1 (MQ=255)

                                                                                                                 tCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGc                                                                                                                  <  2:108254/42‑1 (MQ=255)

                                                                                                                 tCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGc                                                                                                                  >  1:108254/1‑42 (MQ=255)

                                                                                                                               aaaTCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGaaaa   >  1:214958/1‑139 (MQ=255)

                                                                                                                                aaTCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAaag  <  1:727749/139‑1 (MQ=255)

TTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAG  >  NC_000913/2319594‑2319860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A