Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A10 F1 I2 R1
|
67 |
36.0 |
1614110 |
95.1% |
1535018 |
114.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,367,989 |
(C)5→6 |
coding (919/969 nt) |
arnC → |
undecaprenyl phosphate‑L‑Ara4FN transferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,367,984 | 1 | . | C | 100.0%
| 122.2
/ NA
| 35 | R305P (CGC→CCC) | arnC | undecaprenyl phosphate‑L‑Ara4FN transferase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (20/15); total (20/15) |
ACCTTCGGACCACAATGGGCGGCAGAAGGCGTCTTTATGCTATTTGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCG‑CCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCTGCCGGTT > NC_000913/2367848‑2368108
|
aCCTTCGGACCACAATGGGCGGCAGAAGGCGTCTTTATGCTATTTGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGcgcccg‑cc > 2:723973/1‑139 (MQ=255)
ttCGGACCACAATGGGCGGCAGAAGGCGTCTTTATCCTATTTGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCG‑ccccc < 1:487482/139‑1 (MQ=255)
cGGACCACAATGGGCGGCAGAAGGCGTCTTTATGCTATTTGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCG‑ccccccg < 1:723973/139‑3 (MQ=255)
cGGACCACAATGGGCGGCAGAAGGCGTCTTTATGCTATTTGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCG‑ccccccg > 1:460881/1‑137 (MQ=255)
cAGAAGGCGTCTTTATGCTATTTGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCCGCTATTTTGTTCAGCAAg > 1:59859/1‑139 (MQ=255)
aaGGCGTCTTTATGCTATTTGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCCGCTATTTTGTTCAGCAAGTTa < 2:59859/139‑1 (MQ=255)
tCTTTATGCTATTTGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCCGCTATTTTGTTCAGCAAGTTATCCGtc > 2:645660/1‑139 (MQ=255)
gCTATTTGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAg < 2:460881/139‑1 (MQ=255)
tttGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAg > 1:229632/1‑139 (MQ=255)
tttGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAg > 2:94141/1‑139 (MQ=255)
ttGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAgg > 2:725961/1‑139 (MQ=255)
gCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGaa > 2:414535/1‑139 (MQ=255)
ccGTCCTGTTTACTTTTATGGGCGCTAAGTTTTTCGGCATGGTATTACACGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGaaa < 2:606876/139‑1 (MQ=255)
gTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAAt < 2:298906/139‑1 (MQ=255)
ttGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCG‑ccccccg > 1:487725/1‑78 (MQ=255)
ctGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCG‑ccccccg < 2:487725/79‑3 (MQ=255)
gcgcTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACcgtcgt > 1:38406/1‑139 (MQ=255)
cgcTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCCGCTATTTTGTTCAGCAAGTTATCAGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGGCGtt > 2:84554/1‑139 (MQ=255)
gcTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGttt > 1:719595/1‑139 (MQ=255)
gcTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCG‑ccccccg < 2:317862/75‑3 (MQ=255)
gcTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCG‑ccccccg < 1:112829/75‑3 (MQ=255)
gcTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCG‑ccccccg > 2:112829/1‑73 (MQ=255)
gcTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCG‑ccccccg > 1:317862/1‑73 (MQ=255)
tttATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCt < 1:645660/139‑1 (MQ=255)
ggATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATgg > 1:189862/1‑139 (MQ=255)
aTTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGa < 2:229632/139‑1 (MQ=255)
tCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCt < 1:414535/139‑1 (MQ=255)
tGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCCGCTATTTTGtt > 1:515585/1‑56 (MQ=255)
tGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCCGCTATTTTGtt < 2:515585/56‑1 (MQ=255)
tGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGaaa < 2:204589/87‑1 (MQ=255)
tGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGaaa > 1:204589/1‑87 (MQ=255)
tGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGt > 1:642360/1‑139 (MQ=255)
ggCAGGATCTACACCGATGTCCGCGCCCGCCCCCCGCTATTTTGTTc > 2:389592/1‑47 (MQ=255)
ggCAGGATCTACACCGATGTCCGCGCCCGCCCCCCGCTATTTTGTTc < 1:389592/47‑1 (MQ=255)
aTCTACACCGATGTCCGCGCCCGCCCCCCGCTATTTTGTTCAGCAAGTTCTCCGTCCATCCAGCAAGGaaa > 2:88471/1‑71 (MQ=255)
aTCTACACCGATGTCCGCGCCCGCCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGaaa < 1:88471/71‑1 (MQ=255)
gATGTCCGCGCCCGCCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTAc > 1:185641/1‑93 (MQ=255)
gATGTCCGCGCCCGCCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTAc < 2:185641/93‑1 (MQ=255)
gATGTCCGCGCCCGCCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCTGCCGGtt > 2:9693/1‑139 (MQ=255)
aTGTCCGCGCCCGCCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGCAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCt > 2:598176/1‑109 (MQ=255)
aTGTCCGCGCCCGCCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCTTCGTTTTTGCCTACCACGATATGGGATGCCt < 1:598176/109‑1 (MQ=255)
aTGTCCGCGCCCGCCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTAc < 2:622611/92‑1 (MQ=255)
aTGTCCGCGCCCGCCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTAc > 1:622611/1‑92 (MQ=255)
tGTCCGCGCCCGCCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGttt > 2:607173/1‑83 (MQ=255)
tGTCCGCGCCCGCCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGttt < 1:607173/83‑1 (MQ=255)
|
ACCTTCGGACCACAATGGGCGGCAGAAGGCGTCTTTATGCTATTTGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCG‑CCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCTGCCGGTT > NC_000913/2367848‑2368108
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
