Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A10 F1 I2 R1
|
67 |
36.0 |
1614110 |
95.1% |
1535018 |
114.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,657,358 |
G→A |
A526A (GCC→GCT) |
hscA ← |
DnaK‑like molecular chaperone specific for IscU |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,657,358 | 0 | G | A | 100.0%
| 99.9
/ NA
| 29 | A526A (GCC→GCT) | hscA | DnaK‑like molecular chaperone specific for IscU |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/10); total (19/10) |
CAGCATCGTCAATGACCTGACGTTCTGCGGCGCTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGC > NC_000913/2657240‑2657491
|
cagcaTCGTCAATGACCTGACGTTCTGCGGCGCTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGc > 1:637795/1‑139 (MQ=255)
gcaTCGTCAATGACCTGACGTTCTGCGGCGCTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCAt > 1:208324/1‑139 (MQ=255)
aaTGACCTGACGTTCTGCGGCGCTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCAtt < 2:785831/139‑1 (MQ=255)
aaTGACCTGACGTTCTGCGGCGCTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCAtt < 2:208324/139‑1 (MQ=255)
aaTGACCTGACGTTCTGCGGCGCTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCAtt > 1:71876/1‑139 (MQ=255)
aaTGACCTGACGTTCTGCGGCGCTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCAtt < 1:338317/139‑1 (MQ=255)
gACGTTCTGCGGCGCTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCttt > 1:545111/1‑139 (MQ=255)
gcgcTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAg > 1:608622/1‑139 (MQ=255)
cgcTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGc > 1:594894/1‑139 (MQ=255)
cgGCATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATc > 2:138014/1‑139 (MQ=255)
cgGCATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATc > 2:217042/1‑139 (MQ=255)
cagcagcagCCGGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACCTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGt > 2:467092/4‑139 (MQ=255)
tCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCa < 1:408884/139‑1 (MQ=255)
agcagcCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGa < 2:637795/139‑1 (MQ=255)
cgcgcCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCAt < 1:326153/83‑1 (MQ=255)
cgcgcCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCAt > 2:326153/1‑83 (MQ=255)
cgcgcCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGAc > 2:675403/1‑139 (MQ=255)
cGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGttt < 1:138014/139‑1 (MQ=255)
tGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGa > 2:705573/1‑139 (MQ=255)
ccAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGa > 1:342275/1‑139 (MQ=255)
ccAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGa < 2:594894/139‑1 (MQ=255)
ccAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGa > 1:742792/1‑139 (MQ=255)
aGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACg > 1:224644/1‑139 (MQ=255)
acacGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCt > 1:682962/1‑139 (MQ=255)
gcgcCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAAc > 2:540178/1‑139 (MQ=255)
gccgcTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGc < 1:540178/139‑1 (MQ=255)
gcTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCgg < 2:682962/139‑1 (MQ=255)
aTTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGc > 2:358047/1‑139 (MQ=255)
aTTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGc > 1:338331/1‑139 (MQ=255)
|
CAGCATCGTCAATGACCTGACGTTCTGCGGCGCTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGC > NC_000913/2657240‑2657491
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
