Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I2 R1	67	36.0	1614110	95.1%	1535018	114.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,216,464	G→A	R10C (CGC→TGC)	yqjH ←	putative siderophore interacting protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,216,464	0	G	A	100.0%	142.9 / NA	41	R10C (CGC→TGC)	yqjH	putative siderophore interacting protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/25); total (16/25)

GCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATA  >  NC_000913/3216330‑3216592

gCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGc                                                                                                                              >  2:682630/1‑139 (MQ=255)

         gTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCgg                                                                                                                            >  1:100954/1‑132 (MQ=255)

         gTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCgg                                                                                                                            <  2:100954/132‑1 (MQ=255)

          tCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCggg                                                                                                                    <  1:124619/139‑1 (MQ=255)

              aGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGgt                                                                                                                <  2:236421/139‑1 (MQ=255)

                   cgcgATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTAt                                                                                                           >  2:609923/1‑139 (MQ=255)

                      gATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCa                                                                                                        <  1:533441/139‑1 (MQ=255)

                      gATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCa                                                                                                        <  1:682630/139‑1 (MQ=255)

                         gTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCa                                                                                                        <  2:618236/136‑1 (MQ=255)

                         gTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCa                                                                                                        >  1:618236/1‑136 (MQ=255)

                             aaCCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTAt                                                                                                 <  1:191683/139‑1 (MQ=255)

                             aaCCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTAt                                                                                                 <  2:30895/139‑1 (MQ=255)

                                  tCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGc                                                                                                                              >  1:368464/1‑105 (MQ=255)

                                  tCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGc                                                                                                                              <  2:368464/105‑1 (MQ=255)

                                  tCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCggg                                                                                                                    >  1:452444/1‑115 (MQ=255)

                                  tCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCggg                                                                                                                    <  2:452444/115‑1 (MQ=255)

                                  tCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCt                                                                                            >  1:274924/1‑139 (MQ=255)

                                  tCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCt                                                                                            >  2:33962/1‑139 (MQ=255)

                                  tCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCt                                                                                            >  1:740267/1‑139 (MQ=255)

                                         ccTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGAt                                                                                     <  1:609923/139‑1 (MQ=255)

                                          cTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATg                                                                                    <  2:274924/139‑1 (MQ=255)

                                          cTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATg                                                                                    <  1:163197/139‑1 (MQ=255)

                                                   gAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGa                                                                           <  1:749701/139‑1 (MQ=255)

                                                        cAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGatatat                                                                      <  2:251535/139‑1 (MQ=255)

                                                                tGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCtaaataa                                                              >  1:66953/1‑139 (MQ=255)

                                                                  aaaaCCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATaaaa                                                            >  1:297173/1‑139 (MQ=255)

                                                                       cGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGc                                                       >  1:760038/1‑139 (MQ=255)

                                                                              gATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAaatgat                                                <  2:297173/139‑1 (MQ=255)

                                                                                 gcgcTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAaatgataat                                             <  1:411472/139‑1 (MQ=255)

                                                                                                  cagtcagtTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATa                            <  1:96308/139‑1 (MQ=255)

                                                                                                   agtcagtTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGatata                                                                       >  2:53440/1‑95 (MQ=255)

                                                                                                   agtcagtTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGatata                                                                       <  1:53440/95‑1 (MQ=255)

                                                                                                            aCGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCg                  <  1:84093/139‑1 (MQ=255)

                                                                                                              ggAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGAt                <  2:760038/139‑1 (MQ=255)

                                                                                                                   cgcAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATg                                                                                    >  2:355732/1‑66 (MQ=255)

                                                                                                                   cgcAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATg                                                                                    <  1:355732/66‑1 (MQ=255)

                                                                                                                   cgcAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTa           >  1:74285/1‑139 (MQ=255)

                                                                                                                           aTTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGat   <  2:66953/139‑1 (MQ=255)

                                                                                                                            ttGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGata  <  2:176543/139‑1 (MQ=255)

                                                                                                                             tGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCg                                                                                         >  1:534779/1‑51 (MQ=255)

                                                                                                                             tGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCg                                                                                         <  2:534779/51‑1 (MQ=255)

GCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATA  >  NC_000913/3216330‑3216592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A