Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A10 F1 I1 R1
|
77 |
35.1 |
1569012 |
96.6% |
1515665 |
112.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,216,464 |
G→A |
R10C (CGC→TGC) |
yqjH ← |
putative siderophore interacting protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,216,464 | 0 | G | A | 100.0%
| 95.7
/ NA
| 28 | R10C (CGC→TGC) | yqjH | putative siderophore interacting protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (13/15); total (13/15) |
ACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCT > NC_000913/3216348‑3216597
|
aCGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTa < 2:667193/139‑1 (MQ=255)
gTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGcc < 1:277873/139‑1 (MQ=255)
tCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCt > 1:684230/1‑139 (MQ=255)
tCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCt > 1:162793/1‑139 (MQ=255)
cgcCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTg < 1:56715/139‑1 (MQ=255)
cgccgcCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCg > 1:399595/1‑103 (MQ=255)
cgccgcCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCg < 2:399595/103‑1 (MQ=255)
cgccgAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAAt < 2:36615/139‑1 (MQ=255)
gACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTaa > 2:509115/1‑131 (MQ=255)
gACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTaa < 1:509115/131‑1 (MQ=255)
tGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTaa < 2:475546/121‑1 (MQ=255)
tGAAAACCGGCGCTCATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACAGCGGGTAGCGGGGGGTGTTATTCATTATTATCGCCTTCGTGATGGTaa > 1:475546/1‑121 (MQ=255)
cGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTTATGGTAATCAGATATATCTAAATAAAACTCGc < 2:466027/139‑1 (MQ=255)
cGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGc > 1:453145/1‑139 (MQ=255)
agtcagtTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAAt < 1:169078/87‑1 (MQ=255)
agtcagtTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAAt > 2:169078/1‑87 (MQ=255)
agtcagtTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGc < 2:131355/111‑1 (MQ=255)
agtcagtTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGc > 1:131355/1‑111 (MQ=255)
cgcAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGa < 1:658879/64‑1 (MQ=255)
cgcAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGa > 2:658879/1‑64 (MQ=255)
cgcAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGAt < 2:261159/134‑1 (MQ=255)
cgcAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGAt > 1:261159/1‑134 (MQ=255)
cgcAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTa > 1:534382/1‑139 (MQ=255)
cgcAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTa > 2:495805/1‑139 (MQ=255)
cgcAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTa > 2:498957/1‑139 (MQ=255)
cgcAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCACATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTa < 2:534382/139‑1 (MQ=255)
ttGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAGCTGata < 1:498957/139‑1 (MQ=255)
aaCGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCt < 1:649924/139‑1 (MQ=255)
|
ACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCT > NC_000913/3216348‑3216597
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
