Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A10 F1 I2 R1
|
67 |
36.0 |
1614110 |
95.1% |
1535018 |
114.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,131,253 |
G→A |
W473* (TGG→TGA) |
metL → |
Bifunctional aspartokinase/homoserine dehydrogenase 2 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,131,253 | 0 | G | A | 100.0%
| 99.5
/ NA
| 29 | W473* (TGG→TGA) | metL | Bifunctional aspartokinase/homoserine dehydrogenase 2 |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/13); total (16/13) |
GGCATCAGCCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCAGGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTT > NC_000913/4131119‑4131381
|
ggCATCAGCCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGACTgg < 2:359957/139‑1 (MQ=255)
gCCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGACTGGAACTGtt < 1:581843/139‑1 (MQ=255)
aGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTgag < 1:687277/139‑1 (MQ=255)
gTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTgagc > 1:277674/1‑139 (MQ=255)
cgcACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGACTGGAACTGtt > 1:264147/1‑122 (MQ=255)
cgcACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGACTGGAACTGtt < 2:264147/122‑1 (MQ=255)
cGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTGAGCAGAGCACGCtt < 2:796781/139‑1 (MQ=255)
gCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTc > 1:255666/1‑139 (MQ=255)
aaaGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTAc > 1:105415/1‑139 (MQ=255)
gcgcAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGACTGGaa > 1:79563/1‑65 (MQ=255)
gcgcAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGACTGGaa < 2:79563/65‑1 (MQ=255)
gcgcAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCAGGTGTGGTGGACAg > 2:330955/1‑139 (MQ=255)
gcgcAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCAGGTGTGGTGGACAg > 2:240389/1‑139 (MQ=255)
gAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCAGGTGTGGTGGACAgccgcc < 1:217744/139‑1 (MQ=255)
cGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCAGGTGTGGTGGACAGCCGCCGCAGCCTGTTg > 2:306155/1‑139 (MQ=255)
ttGTTCGGTAAGGGCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCAGGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACg < 1:567038/139‑1 (MQ=255)
ttCGGTAAGGGCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCAGGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGc > 2:361821/1‑139 (MQ=255)
gTAAGGGCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCAGGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGa > 1:396865/1‑139 (MQ=255)
gTAAGGGCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCAGGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGa < 1:330955/139‑1 (MQ=255)
tAAGGGCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCt < 1:753052/87‑1 (MQ=255)
tAAGGGCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCt > 2:753052/1‑87 (MQ=255)
tAAGGGCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCAGGTGTGGTGGACAgccgc > 2:677412/1‑109 (MQ=255)
tAAGGGCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCAGGTGTGGTGGACAgccgc < 1:677412/109‑1 (MQ=255)
gggCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCAGGTGTGGTGGACAgccgcc > 1:507830/1‑107 (MQ=255)
gggCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCAGGTGTGGTGGACAgccgcc < 2:507830/107‑1 (MQ=255)
ggCAATATCGGTTCCCGTTGACTGGAACTGTTCGcc < 2:234214/36‑1 (MQ=255)
ggCAATATCGGTTCCCGTTGACTGGAACTGTTCGcc > 1:234214/1‑36 (MQ=255)
gCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCAGGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAg > 2:418047/1‑139 (MQ=255)
gTTCCCGTTGACTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCAGGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGtt > 2:551455/1‑139 (MQ=255)
|
GGCATCAGCCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCAGGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTT > NC_000913/4131119‑4131381
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
